LOCUS       sequence-310158-       13983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual fluorescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 13983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO2112
FEATURES             Location/Qualifiers
     source          1..13983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1614..1634
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1650..1774
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1811..1816,1817..1834,1835..1841)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1895..2554
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1961..1980)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2896..3201
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3080..3099
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3242..3366)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4100..4165
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(4172..4174,4175..4177,4178..4885)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(4217..4238)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4478..4497)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4825..4846
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4920..5172
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5205..5221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5205..5221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5218..5240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5229..5245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5253..5259,5260..5277,5278..5283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5298..5319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5353..5605)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5995..6789)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6106..6125)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6716..6735
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6810..6993)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6846..7025)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7199..7223
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7744..8535
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8781..9369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9270..9289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9523..9540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9555..9695
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9621..9640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9781..9803
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10039..10233
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10299..11372)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11801..12430)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13731..13755
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     13943..13965
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13957..13974
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13958..13974
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgacgtcgta tgatcgcgaa gcacccaccc taagccacat tttaatcctt ctgttggcca
       61 tgccccatca aagttgcact taacccaaga ttgtggtgga gcttcccatg tttctcgtct
      121 gtcccgacgg tgttgtggtt ggtgctttcc ttacattctg agcctctttc cttctaatcc
      181 actcatctgc atcttcttgt gtccttacta atacctcatt ggttccaaat tccctccctt
      241 taagcaccag ctcgtttctg ttcttccaca gcctcccaag tatccaaggg actaaagcct
      301 ccacattctt cagatcagga tattcttgtt taagatgttg aactctatgg aggtttgtat
      361 gaactgatga tctaggaccg gataagttcc cttcttcata gcgaacttat tcaaagaatg
      421 ttttgtgtat cattcttgtt acattgttat taatgaaaaa atattattgg tcattggact
      481 gaacacgagt gttaaatatg gaccaggccc caaataagat ccattgatat atgaattaaa
      541 taacaagaat aaatcgagtc accaaaccac ttgccttttt taacgagact tgttcaccaa
      601 cttgatacaa aagtcattat cctatgcaaa tcaataatca tacaaaaata tccaataaca
      661 ctaaaaaatt aaaagaaatg gataatttca caatatgtta tacgataaag aagttacttt
      721 tccaagaaat tcactgattt tataagccca cttgcattag ataaatggca aaaaaaaaca
      781 aaaaggaaaa gaaataaagc acgaagaatt ccagaaaata cgaaatacgc ttcaatgcag
      841 tgggacccac ggttcaatta ttgccaattt tcagctccac cgtatattta aaaaataaaa
      901 cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa tctcaacggc tggatcttat
      961 gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg
     1021 cagccggcac acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta
     1081 aaaataaggc aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca
     1141 ttttattatt agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct
     1201 tttcttcttc ttcttctata aaacaatacc caaagagttc ttcttcttca caattcagat
     1261 ttcaatttct caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa
     1321 tttctgtgtt ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg
     1381 tatatgttct ttggtttaga ttctgttaat cttagatcga agacgatttt ctgggtttga
     1441 tcgttagata tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa
     1501 ataatttgag ttttgtcgaa taattactct tcgatttgtg atttctatcc agatctggtg
     1561 ttagtttcta gtttgtgcga tcgaatttgt cctcgagagg ggactctaga atgccaaaaa
     1621 agaagagaaa ggtagaagac cccactagta caagtttgta caaaaaagct gaacgagaaa
     1681 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1741 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1801 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1861 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1921 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1981 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2041 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2101 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2161 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2221 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2281 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2341 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2401 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2461 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2521 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2581 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2641 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt
     2701 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2761 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2821 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2881 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2941 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3001 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3061 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     3121 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3181 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     3241 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     3301 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     3361 agtggtgggc ccaatggtgt ccaagggcga agctgtgatt aaagaattta tgcgttttaa
     3421 ggtccacatg gaaggatcca tgaatggaca tgaattcgaa atcgaggggg agggcgaggg
     3481 ccgaccttat gagggtacac agaccgcgaa gctaaaggta accaagggag gcccactacc
     3541 ctttagttgg gacatactca gcccgcagtt tatgtatggc agtcgtgcct ttataaagca
     3601 cccagcggat ataccagact actacaaaca gtccttccca gaggggttta aatgggagcg
     3661 agtgatgaac tttgaggacg gtggtgctgt gacggtaact caggacacat cactcgaaga
     3721 cgggactctt atttacaaag taaaactgag agggaccaat tttccaccag atggccccgt
     3781 catgcaaaaa aagacgatgg gttgggaagc atctacagaa cgactctatc ccgaggacgg
     3841 cgtcctcaaa ggcgacatta aaatggcctt gaggttgaaa gacggtggac gttacttggc
     3901 ggacttcaaa acaacttata aagcaaaaaa gccagtgcaa atgcctggag cgtacaatgt
     3961 agatagaaaa cttgatataa cgtcacataa tgaggactat accgtggttg agcaatacga
     4021 gaggtcagaa ggacgacaca gcaccggcgg tatggacgag ttgtacaaga cgcgtcataa
     4081 gcagaaaatc gttgcaccag ttaagcagac gttgaacttt gatttgctta aactagcagg
     4141 tgatgttgaa tccaatcctg gacctggtac catggtgagc aagggcgagg agctgttcac
     4201 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4261 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
     4321 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca
     4381 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4441 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4501 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4561 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4621 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     4681 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4741 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     4801 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4861 cactctcggc atggacgagc tgtacattgc tgcagcggcc taagagctcg aatttccccg
     4921 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     4981 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     5041 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     5101 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     5161 tgttactaga tcgggaattg gttccggaac caattcgtaa tcatgtcata gctgtttcct
     5221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5281 aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat
     5341 ttctgaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     5401 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     5461 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     5521 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     5581 tttattgcca aatgtttgaa cgatcgggga tcatccgggt ctgtggcggg aactccacga
     5641 aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc
     5701 cgacgccgtt gatgtggacg ccgggcccga tcatattgtc gctcaggatc gtggcgttgt
     5761 gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt tccgcagaga
     5821 tcccgtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
     5881 cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
     5941 gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa
     6001 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     6061 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     6121 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     6181 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     6241 atcatcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     6301 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     6361 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     6421 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     6481 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     6541 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     6601 ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     6661 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     6721 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     6781 ttcaatcatg cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga
     6841 gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa
     6901 atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac
     6961 gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc
     7021 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     7081 tgactccctt aattctccgc tcatgatgaa ttcagaaata aattcagcct aattcggcgt
     7141 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     7201 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     7261 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     7321 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     7381 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     7441 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     7501 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     7561 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     7621 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     7681 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     7741 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     7801 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     7861 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     7921 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     7981 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     8041 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     8101 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     8161 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     8221 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     8281 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     8341 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     8401 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     8461 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     8521 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
     8581 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     8641 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     8701 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8761 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8821 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8881 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8941 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9001 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9061 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9121 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9301 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9361 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9421 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9481 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9541 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9601 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     9661 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     9721 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     9781 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     9841 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     9901 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     9961 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
    10021 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
    10081 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
    10141 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
    10201 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
    10261 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
    10321 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
    10381 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
    10441 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
    10501 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
    10561 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
    10621 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
    10681 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
    10741 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
    10801 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
    10861 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
    10921 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
    10981 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
    11041 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
    11101 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
    11161 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
    11221 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
    11281 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
    11341 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
    11401 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
    11461 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    11521 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    11581 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    11641 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    11701 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    11761 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    11821 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    11881 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    11941 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    12001 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    12061 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    12121 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    12181 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    12241 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    12301 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    12361 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    12421 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    12481 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    12541 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    12601 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    12661 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    12721 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    12781 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    12841 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    12901 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    12961 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    13021 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    13081 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    13141 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    13201 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    13261 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    13321 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    13381 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    13441 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    13501 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    13561 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    13621 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt
    13681 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc
    13741 caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc
    13801 aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga agggcgatcg
    13861 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    13921 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    13981 ctt
//
