LOCUS       sequence-310209-       12620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce a constitutive rtta3G cassette and a 
            DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE 
            acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12620)
  AUTHORS   Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, 
            Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan 
            M, Bruneau BG
  TITLE     Molecular basis of CTCF binding polarity in genome folding.
  JOURNAL   Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x.
  PUBMED    33154377
REFERENCE   2  (bases 1 to 12620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-5"; volume: "11(1):5612. doi"; pages: " 
            10.1038/s41467-020-19283-x"
COMMENT     Sequence Label: pAU002 -
            pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-...
FEATURES             Location/Qualifiers
     source          1..12620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1378..1727
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1386..1404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1422..1440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1458..1476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1494..1512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1566..1584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1602..1620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1666..1690
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1842..1907
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3348..4058
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4080..4304
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4337..4472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    4577..4598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4613..4618,4619..4636,4637..4643)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4651..4667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4675..4691
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5034..5053
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5052..5107
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5153..5172
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5251..5997)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKGKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6094..6113)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6121..7137
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6175..6197)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        7415..7718
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    7782..7815
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7914..7937)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8287..8306
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        8366..8413
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8432..9031
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8432..8451)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8928..8948
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9069..9293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9348..9381
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(10399..10415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10423..10439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10447..10453,10454..10471,10472..10477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10492..10513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10630..10647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10801..11389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10881..10900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11560..12351,12352..12420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12183..12202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12421..12525)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctgcaggg tatcacgagg ccctttcgtc ttcactcgag
     1381 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
     1441 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
     1501 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
     1561 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
     1621 acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     1681 ccgtcagatc gcctggagca attccacaac acttttgtct tatacttacg cgtctgcagg
     1741 atatcaagct tgcggtaccg cggccccgaa ttcgagctcg gtacccgggg atcctctagt
     1801 cagctgacgc gtgctagcgc ggccgcatcg attccaccat ggactataag gaccacgacg
     1861 gagactacaa ggatcatgat attgattaca aagacgatga cgataaggtc gacttccagt
     1921 gtgagctttg cagttacaca tgtccccggc gttcaaattt ggatcgtcac atgaaaagcc
     1981 acactgatga gagaccacac aaatgccatc tgtgtggcag agcattcaga acagtgaccc
     2041 tcctgaggaa tcatctgaac acacacacag gtactcgtcc tcacaagtgc ccagactgcg
     2101 atatggcctt tgtgaccagt ggagaattgg tgcggcatcg tcgttataaa cacactcatg
     2161 agaaaccatt taagtgttcc atgtgtgatt atgccagtgt agaagtcagc aaattaaaac
     2221 gacacattcg ctctcatact ggagagcgcc cgttccagtg cagtttgtgc agttatgcca
     2281 gcagggacac atacaagctg aaaaggcata tgagaaccca ttcaggggaa aaaccttatg
     2341 aatgttatat ttgtcacgct cggtttaccc agagtggtac catgaagatg cacattttac
     2401 agaagcacac agaaaatgtg gccaaatttc attgtcccca ttgtgacact gtcatagccc
     2461 gaaaaagtga tttgggtgtc cacttgcgaa agcagcattc ctatattgaa cagggcaaaa
     2521 aatgtcgcta ctgtgatgct gtgtttcatg agcgatatgc tctcatccag catcaaaaat
     2581 cacacaaaaa tgagaagcgc ttcaagtgtg accagtgtga ttatgcttgt agacaggagc
     2641 ggcacatgat catgcacaag cgcactcaca cgggggagaa gccttatgcc tgcagccact
     2701 gcgacaagac cttccgccag aaacagctcc tcgacatgca tttcaagcgc tatcatgatc
     2761 ccaactttgt ccctgctgcc tttgtctgtt ccaagtgtgg gaaaacattc acccgccgga
     2821 acacaatggc aagacatgca gataactgtg ctggtccaga tggcgtagag ggggaaaatg
     2881 gaggggagac aaagaagagc aaacgaggaa gaaaaagaaa gatgcgatct aaaaaagaag
     2941 actcctctga cagtgaagaa aatgctgagc cggatctcga tgacaatgag gaggaggagg
     3001 agcctgctgt agaaattgaa cctgagccag agcctcagcc ccagcctccg cctccacctc
     3061 agcctgtggc cccggccccc ccacctgcca agaagagaag ggggcgacct cctggaagaa
     3121 ccaaccagcc caaacagaac cagccaacag ccatcattca ggtcgaagat cagaatacag
     3181 gtgcaattga gaacattata gttgaagtca aaaaggagcc agatgccgag cctgcggagg
     3241 gggaagaaga ggaggctcag gcagccacca cagacgcccc caacggagac ctcacgcctg
     3301 agatgatcct cagcatgatg gaccggctta aggggggagc aggaggcatg gtgtctaagg
     3361 gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg
     3421 gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca
     3481 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt
     3541 cgttcatgta tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta
     3601 aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag
     3661 tcgtcaccgt catgcaggac accagccttg aggatggctg tctcgtttac cacgtccaag
     3721 tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg
     3781 agcctaatac agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg
     3841 cactgaaagt tgatggtggt ggccatctgt cttgctcttt cgtaacaact tacaggtcaa
     3901 aaaagaccgt cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa
     3961 ggttagagga aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt
     4021 tcgccgggct tggtggtggg atggacgagc tgtacaagta agtttaaaca tagcctcgac
     4081 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4141 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4201 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4261 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4321 aaccttaatt aacctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     4381 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     4441 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     4501 gctgactaat tgagatgcgg atccgctggc acgacaggtt tcccgactgg aaagcgggca
     4561 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4621 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4681 acagctatga ccatgattac gccaagcttg ggctgcaggt cgagggatct ccataagaga
     4741 agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc tagtaaaaca
     4801 tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa tataaaaaaa
     4861 atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg gggctgtttc
     4921 atatactgat gacctcttta tagccaacct ttgttcatgg cagccagcat atgggcatat
     4981 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     5041 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     5101 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     5161 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     5221 ttctgatagg cagcctgcac ctgaggccgc ttacccgggg agcatgtcaa ggtcaaaatc
     5281 gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag
     5341 catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg
     5401 tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg
     5461 ccggtcgaac agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt
     5521 ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa
     5581 gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag
     5641 gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg
     5701 cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg
     5761 cagaaagtct tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag
     5821 catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt
     5881 aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac
     5941 tccattgagt aattccagag cagagtttat gactttgccc ttgtccagtc tagacatggt
     6001 ggcggcggat cccgggcccg cggtaccgca agcttgatat cctgcagacg cgtgagaatt
     6061 ctcgaggaat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag
     6121 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
     6181 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
     6241 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc
     6301 cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt
     6361 gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc
     6421 cctctcgcac gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc
     6481 ggcgctccgg gggccgccgc gcccctcccc cgagccctcc ccggcccgag gcggccccgc
     6541 cccgcccggc acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg
     6601 ccccgtacgg agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg
     6661 cagggggggt tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac
     6721 accccgcacg cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc
     6781 ggccgcgctc ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg
     6841 ctcacagccg ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca
     6901 cgcacccccc gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc
     6961 acgcagccac agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg
     7021 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     7081 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc
     7141 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     7201 ctttcggagc gcgccgctct gattggctgc cgccgcacct ctccgcctcs ccccsccccs
     7261 ccccycsccc csccccsccc csccyggcgc gcsccccccc cccccccgcc cccatcgctg
     7321 cacaaaataa ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg
     7381 ggagagtgaa gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt
     7441 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     7501 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     7561 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     7621 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     7681 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     7741 gaactaatga ccccgtaatt gattactatt aataactagt cgaagttcct attctctaga
     7801 aagtatagga acttcaggtc tgaagaggag tttacgtcca gccaagctag cttggctgca
     7861 ggtcgtcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     7921 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     7981 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     8041 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     8101 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     8161 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     8221 ctcagaggct gggaaggggt gggtccgggg gcggggcggg cgcccgaagg tcctccggag
     8281 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     8341 ctccgggcct ttcgacctgc agcctgttga caattaatca tcggcatagt atatcggcat
     8401 agtataatac gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct
     8461 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     8521 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     8581 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     8641 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     8701 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     8761 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     8821 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     8881 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     8941 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     9001 ctggtgcatg acccgcaagc ccggtgcctg aggggatcaa ttctctagag ctcgctgatc
     9061 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9121 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9181 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9241 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     9301 ggcggaaaga accagctggg gctcgactag agcttgcgga acccttcgaa gttcctattc
     9361 tctagaaagt ataggaactt cggcgcgcca tttaaatttt gggatgtttt ctgtttgttt
     9421 tgagataggg tttttcagtg tagtcctggc tgtcctagaa ctcactctgt agaccaggct
     9481 ggcctggaac tcaaggactg ccagcctctg cctcctgagc atcgggatta aaggtgtgtg
     9541 ccaccactgc ccagcagagt gcgtttttac tcttcacttt gtgggtcctg gctgcctgag
     9601 attgtgtgct tgcaccatta gatgggaggg atgacttggg aaagggttga gtcttttgtg
     9661 agtcttgcgg aggcccagga gggggtacgt aaattagagt tctaagaggc ccagcaaaag
     9721 tgaacaagtg actggccact gtgagttcac tgggggacct gagagttaac tagtgaaaag
     9781 gagaggtcct agtggagaag ctaaagaatt aaagttactg ccttgaagtt ctttgtgaag
     9841 gccaagctct ggagcgcggc ttgaaaagcc tttatcccag agcagcatgg gaaggggccc
     9901 cagctgggtg cgagcctgca aaccagagca cacctatcac agggcctttt cttaccagtg
     9961 ggacgggatc aaggcggagc ttacctggga acgtacaggg cggaacctta acttgggggc
    10021 gtggccgaat cacctgggtt tggaaacggt tcttgtcttt cctcaagctc aggagaggtt
    10081 gtttgcagga cactggcccg gaggggcccg aactctccga gggagggttt cgtggatcag
    10141 aacaccgtcc cttgacaaaa agatgcagag caccgtctgc attctgctag taaccgtccc
    10201 tcctgggggc ggagccagcc ctcctccggg tgtataaagt agctgagcgc ctccttctga
    10261 caaacgcctc acccccaaga gtactggggg agtacgcagc acgccagcct aggggcggag
    10321 ctcagccctg gaggaggcgt ttcttcttag ttctccgact cacgcctctc cacttttcat
    10381 ttaaatggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10441 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10501 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11221 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12301 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12541 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    12601 tatcacgagg ccctttcgtc
//
