LOCUS       sequence-310218-        2939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term 6xHis 3C cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 2 pCOLI_A_His
FEATURES             Location/Qualifiers
     source          1..2939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             96..118
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             138..155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             168..191
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(384..402)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      398..445
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        510..601
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1466..1505
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1536..2124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2025..2044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2278..2295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2310..2449
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2375..2394)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2535..2557
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2551..2742)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agatgtaggt gttggcacca tgcatcgatc ccgcgaaatt aatacgactc actataggga
       61 gaccacaacg gtttccctcc ggctccggaa ataattttgt ttaactttaa gaaggagata
      121 tacatatggg cagcagccat catcatcatc atcacagcag cggatctctg gaagttctgt
      181 tccaggggcc cggatcccgg tccgacatat gagcgcgcgg aattcaaagg cctacgtcga
      241 cgagctcact agtcgcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg
      301 agaagtacta gaggatgtac aacgcgtgcg cgctgatccg gctgctaaca aagcccgaaa
      361 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      421 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatcgggat tagcccgcct
      481 aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat
      541 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      601 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      661 tgtttttgct cacccagaaa cgctcgtgaa agtaaaagac gcagaggacc aattgggggc
      721 acgagtggga tacatagaac tggacttgaa tagcggtaaa atccttgaga gttttcgccc
      781 tgaagagcgt tttccaatga tgagcacttt caaagttctg ctatgtggag cagtattatc
      841 ccgtgtagat gcggggcaag agcaactcgg acgacgaata cactattcgc agaatgactt
      901 ggttgaatac tccccagtga cagaaaagca ccttacggac ggaatgacgg taagagaatt
      961 atgtagtgcc gccataacga tgagtgataa cactgcggcg aacttacttc tgacaaccat
     1021 cggtggaccg aaggaattaa ccgctttttt gcacaatatg ggagaccatg taactcgcct
     1081 tgaccgttgg gaaccagaac tgaatgaagc cataccaaac gacgagcgag acaccacaat
     1141 gcctgcggca atggcaacaa cattacgcaa actattaact ggcgaactac ttactctggc
     1201 ttcacggcaa caattaatag actggcttga agcggataaa gttgcaggac cactactgcg
     1261 ttcggcactt cctgctggct ggtttattgc tgataaatct ggggcaggag agcgtggttc
     1321 acggggtatc attgccgcac ttggaccaga tggtaagcct tcccgtatcg tagttatcta
     1381 cacgacgggt agtcaggcaa ctatggacga acgaaataga cagattgctg aaataggggc
     1441 ttcactgatt aagcattggt aaaccgatac aattaaaggc tccttttgga gccttttttt
     1501 ttggacggac cggtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1681 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2281 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     2341 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2401 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     2461 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2521 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2581 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     2641 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     2701 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     2761 gggatttctg ttcatggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     2821 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     2881 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaatac
//
