LOCUS       sequence-310270-       13412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce a constitutive rtta3G cassette and a 
            DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point 
            mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene
            #92144 sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13412)
  AUTHORS   Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, 
            Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan 
            M, Bruneau BG
  TITLE     Molecular basis of CTCF binding polarity in genome folding.
  JOURNAL   Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x.
  PUBMED    33154377
REFERENCE   2  (bases 1 to 13412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-5"; volume: "11(1):5612. doi"; pages: " 
            10.1038/s41467-020-19283-x"
COMMENT     Sequence Label: pEN515 -
            pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3...
FEATURES             Location/Qualifiers
     source          1..13412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1378..1727
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1386..1404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1422..1440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1458..1476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1494..1512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1566..1584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1602..1620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1666..1690
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1842..1907
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4140..4850
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4872..5096
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5129..5264
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    5369..5390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5405..5410,5411..5428,5429..5435)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5443..5459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5467..5483
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5826..5845
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5844..5899
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5945..5964
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6043..6789)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKGKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6886..6905)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6913..7929
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6967..6989)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        8207..8510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8574..8607
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8706..8729)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9079..9098
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9158..9205
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9224..9823
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9224..9243)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9720..9740
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9861..10085
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    10140..10173
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(11191..11207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11215..11231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11239..11245,11246..11263,11264..11269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11284..11305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11422..11439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11593..12181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11673..11692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12352..13143,13144..13212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12975..12994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13213..13317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctgcaggg tatcacgagg ccctttcgtc ttcactcgag
     1381 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
     1441 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
     1501 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
     1561 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
     1621 acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     1681 ccgtcagatc gcctggagca attccacaac acttttgtct tatacttacg cgtctgcagg
     1741 atatcaagct tgcggtaccg cggccccgaa ttcgagctcg gtacccgggg atcctctagt
     1801 cagctgacgc gtgctagcgc ggccgcatcg attccaccat ggactataag gaccacgacg
     1861 gagactacaa ggatcatgat attgattaca aagacgatga cgataaggtc gacgaaggtg
     1921 aggcggttga agccattgtg gaggagtctg aaactttcat taaaggaaaa gaaagaaaga
     1981 cttaccagag acgccgggaa gggggccagg aagaagatgc ttgccacctg ccccagaacc
     2041 agacagatgg gggtgaggtg gtccaggatg tcaacagcag tgtacagatg gtaatgatgg
     2101 aacagctgga tcctaccctt ctccagatga agactgaagt catggagggt acagtggctc
     2161 cggaagcaga ggctgcagtg gacgataccc agatcataac cttgcaggtt gtaaatatgg
     2221 aggaacagcc cattaacata ggagagcttc agcttgtcca agtacctgtt cctgtgacgg
     2281 tacctgttgc tactacttca gtagaagaac ttcagggggc ttatgagaat gaagtgtcta
     2341 aagagggcct tgcagaaagt gaaccgatga tatgtcacac cttacctttg cctgaaggat
     2401 ttcaggtggt gaaagtgggg gccaatggag aagtggagac actagagcag ggcgagcttc
     2461 ctcctcagga agactctagc tggcaaaaag acccagacta tcagccacca gccaaaaaaa
     2521 caaagaaaac caaaaagagc aaacttcgtt acacagaaga gggcaaagac gtggatgtgt
     2581 ctgtgtatga ttttgaggaa gaacagcagg aaggactgct gtctgaggtt aatgcagaga
     2641 aagtagttgg taatatgaag cctccgaagc caacaaaaat taaaaaaaaa ggtgtaaaga
     2701 aaacattcca gtgtgagctt tgcagttaca catgtccccg gcgttcaaat ttggatcgtc
     2761 acatgaaaag ccgaactgat gagagaccac acaaatgcca tctgtgtggc agagcattca
     2821 gaacagtgac cctcctgagg aatcatctga acacacgaac aggtactcgt cctcacaagt
     2881 gcccagactg cgatatggcc tttgtgacca gtggagaatt ggtgcggcat cgtcgttata
     2941 aacacactcg agagaaacca tttaagtgtt ccatgtgtga ttatgccagt gtagaagtca
     3001 gcaaattaaa acgacacatt cgctctcgaa ctggagagcg cccgttccag tgcagtttgt
     3061 gcagttatgc cagcagggac acatacaagc tgaaaaggca tatgagaacc cgatcagggg
     3121 aaaaacctta tgaatgttat atttgtcacg ctcggtttac ccagagtggt accatgaaga
     3181 tgcacatttt acagaagcga acagaaaatg tggccaaatt tcattgtccc cattgtgaca
     3241 ctgtcatagc ccgaaaaagt gatttgggtg tccacttgcg aaagcagcga tcctatattg
     3301 aacagggcaa aaaatgtcgc tactgtgatg ctgtgtttca tgagcgatat gctctcatcc
     3361 agcatcaaaa atcacgaaaa aatgagaagc gcttcaagtg tgaccagtgt gattatgctt
     3421 gtagacagga gcggcacatg atcatgcaca agcgcactcg aacgggggag aagccttatg
     3481 cctgcagcca ctgcgacaag accttccgcc agaaacagct cctcgacatg catttcaagc
     3541 gctatcgaga tcccaacttt gtccctgctg cctttgtctg ttccaagtgt gggaaaacat
     3601 tcacccgccg gaacacaatg gcaagacgag cagataactg tgctggtcca gatggcgtag
     3661 agggggaaaa tggaggggag acaaagaaga gcaaacgagg aagaaaaaga aagatgcgat
     3721 ctaaaaaaga agactcctct gacagtgaag aaaatgctga gccggatctc gatgacaatg
     3781 aggaggagga ggagcctgct gtagaaattg aacctgagcc agagcctcag ccccagcctc
     3841 cgcctccacc tcagcctgtg gccccggccc ccccacctgc caagaagaga agggggcgac
     3901 ctcctggaag aaccaaccag cccaaacaga accagccaac agccatcatt caggtcgaag
     3961 atcagaatac aggtgcaatt gagaacatta tagttgaagt caaaaaggag ccagatgccg
     4021 agcctgcgga gggggaagaa gaggaggctc aggcagccac cacagacgcc cccaacggag
     4081 acctcacgcc tgagatgatc ctcagcatga tggaccggct taagggggga gcaggaggca
     4141 tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag
     4201 gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaat ccgtacatgg
     4261 gaactcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt gcctttgaca
     4321 ttcttgccac gtcgttcatg tatggcagcc gtacttttat caagtacccg aaaggcattc
     4381 ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt acgagatacg
     4441 aagatggtgg agtcgtcacc gtcatgcagg acaccagcct tgaggatggc tgtctcgttt
     4501 accacgtcca agtcagaggg gtaaactttc cctccaatgg tcccgtgatg cagaagaaga
     4561 ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt ctgaggggat
     4621 acactcatat ggcactgaaa gttgatggtg gtggccatct gtcttgctct ttcgtaacaa
     4681 cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtatccat gccgttgatc
     4741 accgcctgga aaggttagag gaaagtgaca atgaaatgtt cgtagtacaa cgcgaacacg
     4801 cagttgccaa gttcgccggg cttggtggtg ggatggacga gctgtacaag taagtttaaa
     4861 catagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4921 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4981 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5041 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5101 tgaggcggaa agaaccttaa ttaacctagg cctccaaaaa agcctcctca ctacttctgg
     5161 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     5221 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     5281 gggactatgg ttgctgacta attgagatgc ggatccgctg gcacgacagg tttcccgact
     5341 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5401 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5461 ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag gtcgagggat
     5521 ctccataaga gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg
     5581 gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa
     5641 aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga atggacagca
     5701 gggggctgtt tcatatactg atgacctctt tatagccaac ctttgttcat ggcagccagc
     5761 atatgggcat atgttgccaa actctaaacc aaatactcat tctgatgttt taaatgattt
     5821 gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac tattgcaatg
     5881 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
     5941 taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc attggccaca
     6001 ccagccacca ccttctgata ggcagcctgc acctgaggcc gcttacccgg ggagcatgtc
     6061 aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc
     6121 atcggctggg agcatgtcta agtcaaaatc gtcaagggcg tcggtcggcc cgccgctttc
     6181 gcactttagc tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg
     6241 ttcggctccc tgccggtcga acagctcaat tgcttgtttc agaagtgggg gcatagaatc
     6301 ggtggtaggt gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca
     6361 gcccagtgta aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc
     6421 ttgctgacac aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg
     6481 ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg agagcacagc ggtatgactt
     6541 ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc agggggcagg agtgggtatg
     6601 atgcctgtcc agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg
     6661 ccagtacagg gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc
     6721 ttcgataccg actccattga gtaattccag agcagagttt atgactttgc ccttgtccag
     6781 tctagacatg gtggcggcgg atcccgggcc cgcggtaccg caagcttgat atcctgcaga
     6841 cgcgtgagaa ttctcgagga attctttgcc aaaatgatga gacagcacaa taaccagcac
     6901 gttgcccagg agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga
     6961 gccgccggtc acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc
     7021 gtccccccgc ggacagcccc gaggctggag agggagaagg ggacggcggc gcggcgacgc
     7081 acgaaggccc tccccgccca tttccttcct gccggcgccg caccgcttcg ccccgcgccc
     7141 gctagagggg gtgcggcggc gcctcccaga tttcggctcc gcacagattt gggacaaagg
     7201 aagtccctgc gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc
     7261 tcgacagccg ccggcgctcc gggggccgcc gcgcccctcc cccgagccct ccccggcccg
     7321 aggcggcccc gccccgcccg gcacccccac ctgccgccac cccccgcccg gcacggcgag
     7381 ccccgcgcca cgccccgtac ggagccccgc acccgaagcc gggccgtgct cagcaactcg
     7441 gggagggggg tgcagggggg gttgcagccc gaccgacgcg cccacacccc ctgctcaccc
     7501 ccccacgcac acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg
     7561 ggcaccgccc ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc
     7621 gcgcccgcag cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca
     7681 cacacacacg cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc
     7741 ctcaaggctt tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt
     7801 acagcccgga ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca
     7861 gagccggggc gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc
     7921 agcgactccc gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc
     7981 cataaaagga aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct
     8041 csccccsccc csccccycsc cccsccccsc cccsccyggc gcgcsccccc cccccccccg
     8101 cccccatcgc tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg
     8161 ggggggagat ggggagagtg aagcagaacg tggggctcac ctcgaccatg gtaatagcga
     8221 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     8281 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     8341 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     8401 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     8461 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     8521 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcgaagttc
     8581 ctattctcta gaaagtatag gaacttcagg tctgaagagg agtttacgtc cagccaagct
     8641 agcttggctg caggtcgtcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt
     8701 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     8761 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     8821 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     8881 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     8941 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     9001 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcggggcg ggcgcccgaa
     9061 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     9121 cctcttcctc atctccgggc ctttcgacct gcagcctgtt gacaattaat catcggcata
     9181 gtatatcggc atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc
     9241 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     9301 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     9361 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     9421 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     9481 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     9541 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     9601 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     9661 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     9721 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     9781 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaggggatc aattctctag
     9841 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     9901 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     9961 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    10021 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    10081 tatggcttct gaggcggaaa gaaccagctg gggctcgact agagcttgcg gaacccttcg
    10141 aagttcctat tctctagaaa gtataggaac ttcggcgcgc catttaaatt ttgggatgtt
    10201 ttctgtttgt tttgagatag ggtttttcag tgtagtcctg gctgtcctag aactcactct
    10261 gtagaccagg ctggcctgga actcaaggac tgccagcctc tgcctcctga gcatcgggat
    10321 taaaggtgtg tgccaccact gcccagcaga gtgcgttttt actcttcact ttgtgggtcc
    10381 tggctgcctg agattgtgtg cttgcaccat tagatgggag ggatgacttg ggaaagggtt
    10441 gagtcttttg tgagtcttgc ggaggcccag gagggggtac gtaaattaga gttctaagag
    10501 gcccagcaaa agtgaacaag tgactggcca ctgtgagttc actgggggac ctgagagtta
    10561 actagtgaaa aggagaggtc ctagtggaga agctaaagaa ttaaagttac tgccttgaag
    10621 ttctttgtga aggccaagct ctggagcgcg gcttgaaaag cctttatccc agagcagcat
    10681 gggaaggggc cccagctggg tgcgagcctg caaaccagag cacacctatc acagggcctt
    10741 ttcttaccag tgggacggga tcaaggcgga gcttacctgg gaacgtacag ggcggaacct
    10801 taacttgggg gcgtggccga atcacctggg tttggaaacg gttcttgtct ttcctcaagc
    10861 tcaggagagg ttgtttgcag gacactggcc cggaggggcc cgaactctcc gagggagggt
    10921 ttcgtggatc agaacaccgt cccttgacaa aaagatgcag agcaccgtct gcattctgct
    10981 agtaaccgtc cctcctgggg gcggagccag ccctcctccg ggtgtataaa gtagctgagc
    11041 gcctccttct gacaaacgcc tcacccccaa gagtactggg ggagtacgca gcacgccagc
    11101 ctaggggcgg agctcagccc tggaggaggc gtttcttctt agttctccga ctcacgcctc
    11161 tccacttttc atttaaatgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    11221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    11341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    11401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    11461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    11521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    11581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    11641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    11701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    11761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    11821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    11881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    11941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12001 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    12061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    12361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    12421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    12481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    12541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    12601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    12661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    12721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    12781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    12841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    12901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    12961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    13321 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    13381 taaaaatagg cgtatcacga ggccctttcg tc
//
