LOCUS       sequence-310396-       13243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Miller CA 3rd, Tan X, Wilson M, Bhattacharyya S, Ludwig S
  TITLE     Single plasmids expressing human steroid hormone receptors and a 
            reporter gene for use in yeast signaling assays.
  JOURNAL   Plasmid. 2010 Mar;63(2):73-8. doi: 10.1016/j.plasmid.2009.11.003. 
            Epub 2009 Dec 3.
  PUBMED    19962400
REFERENCE   2  (bases 1 to 13243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2009.11.003"; journalName: "Plasmid"; date: 
            "2010-03"; volume: "63"; issue: "2"; pages: "73-8"
COMMENT     Sequence Label: pRR-MR-5Z
FEATURES             Location/Qualifiers
     source          1..13243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(36..710)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(711..858)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     promoter        join(864..869,870..887,888..894)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    902..918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     926..942
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(992..1179)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        1349..2013
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    1565..1682
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1977..2000
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(5376..5395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5495..5517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5555..5573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5614..6117
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6154..6258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6259..6327,6328..7119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6477..6496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7290..7878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7779..7798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8032..8049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8166..8187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8202..8207,8208..8225,8226..8232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8240..8256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8264..8280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      12199..12386
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(12538..12557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12539..12557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12564..12581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12564..12580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12573..12595)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12721..13176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12808..12827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13018..13039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa
       61 tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca
      121 ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct
      181 aacataaaat gtaagctttc ggggctctct tgccttccaa cccagtcaga aatcgagttc
      241 caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga
      301 ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta
      361 ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg
      421 acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga
      481 aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt
      541 gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata
      601 cccagcaagt cagcatcgga atctagagca cattctgcgg cctctgtgct ctgcaagccg
      661 caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct
      721 gcctttgtgt gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct
      781 tggccctctc cttttctttt ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc
      841 tccggatcaa gattgtacca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      901 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      961 ttcatagggt aggggaattt cgaccggcat gccggtagag gtgtggtcaa taagagcgac
     1021 ctcatgctat acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa
     1081 ttttcgtttt aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac
     1141 ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag tgtcaacaac
     1201 gtatctacca acgatttgac ccttttccat cttttcgtaa atttctggca aggtagacaa
     1261 gccgacaacc ttgattggag acttgaccaa acctctggcg aagaagtcca aagcttgcat
     1321 gcctgcaggt cgactctaga ggatcccctt tcaaaaattc ttactttttt tttggatgga
     1381 cgcaaagaag tttaataatc atattacatg gcattaccac catatacata tccatataca
     1441 tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat tatcttagcc
     1501 taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt gctatattga
     1561 agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact ctcctccgtg
     1621 cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc
     1681 cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt
     1741 aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag gatgataatg
     1801 cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg atttttgatc
     1861 tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac tttcaacatt
     1921 ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa attgttaata
     1981 tacctctata ctttaacgtc aaggagaaaa aacccgggat ggaagttcaa ttgggtttga
     2041 gagtttaccc aatggagacc aaaggctacc acagtctccc tgaaggtcta gatatggaaa
     2101 gacggtgggg tcaagtttct caggctgtgg agcgttcttc cctgggacct acagagagga
     2161 ccgatgagaa taactacatg gagattgtca acgtaagctg tgtttccggt gctattccaa
     2221 acaacagtac tcaaggaagc agcaaagaaa aacaagaact actcccttgc cttcagcaag
     2281 acaataatcg gcctgggatt ttaacatctg atattaaaac tgagctggaa tctaaggaac
     2341 tttcagcaac tgtagctgag tccatgggtt tatatatgga ttctgtaaga gatgctgact
     2401 attcctatga gcagcagaac caacaaggaa gcatgagtcc agctaagatt tatcagaatg
     2461 ttgaacagct ggtgaaattt tacaaaggaa atggccatcg tccttccact ctaagttgtg
     2521 tgaacacgcc cttgagatca tttatgtctg actctgggag ctccgtgaat ggtggcgtca
     2581 tgcgcgccat tgttaaaagc cctatcatgt gtcatgagaa aagcccgtct gtttgcagcc
     2641 ctctgaacat gacatcttcg gtttgcagcc ctgctggaat caactctgtg tcctccacca
     2701 cagccagctt tggcagtttt ccagtgcaca gcccaatcac ccagggaact cctctgacat
     2761 gctcccctaa tgctgaaaat cgaggctcca ggtcgcacag ccctgcacat gctagcaatg
     2821 tgggctctcc tctctcaagt ccgttaagta gcatgaaatc ctcaatttcc agccctccaa
     2881 gtcactgcag tgtaaaatct ccagtctcca gtcccaataa tgtcactctg agatcctctg
     2941 tgtctagccc tgcaaatatt aacaactcaa ggtgctctgt ttccagccct tcgaacacta
     3001 ataacagatc cacgctttcc agtccggcag ccagtactgt gggatctatc tgtagccctg
     3061 taaacaatgc cttcagctac actgcctctg gcacctctgc tggatccagt acattgcggg
     3121 atgtggttcc cagtccagac acgcaggaga aaggtgctca agaggtccct tttcctaaga
     3181 ctgaggaagt agagagtgcc atctcaaatg gtgtgactgg ccagcttaat attgtccagt
     3241 acataaaacc agaaccagat ggagctttta gcagctcatg tctaggagga aatagcaaaa
     3301 taaattcgga ttcttcattc tcagtaccaa taaagcaaga atcaaccaag cattcatgtt
     3361 caggcacctc ttttaaaggg aatccaacag taaacccgtt tccatttatg gatggctcgt
     3421 atttttcctt tatggatgat aaagactatt attccctatc aggaatttta ggaccacctg
     3481 tgcccggctt tgatggtaac tgtgaaggca gcggattccc agtgggtatt aaacaagaac
     3541 cagatgacgg gagctattac ccagaggcca gcatcccttc ctctgctatt gttggggtgc
     3601 attcaggtgg acagtccttc cactacagga ttggtgctca aggtacaata tctttatcac
     3661 gatcggctag agaccaatct ttccaacacc tgagttcctt tcctcctgtc aatactttag
     3721 tggagtcatg gaaatcacac ggcgacctgt cgtctagaag aagtgatggg tatccggtct
     3781 tagaatacat tccagaaaat gtatcaagct ctactttacg aagtgtttct actggatctt
     3841 caagaccttc aaaaatatgt ttggtgtgtg gggatgaggc ttcaggatgc cattatgggg
     3901 tagtcacctg tggcagctgc aaagttttct tcaaaagagc agtggaaggg caacacaact
     3961 atttatgtgc tggaagaaat gattgcatca ttgataagat tcgacgaaag aattgtcctg
     4021 cttgcagact tcagaaatgt cttcaagctg gaatgaattt aggagcacga aagtcaaaga
     4081 agttgggaaa gttaaaaggg attcacgagg agcagccaca gcagcagcag cccccacccc
     4141 cacccccacc cccgcaaagc ccagaggaag ggacaacgta catcgctcct gcaaaagaac
     4201 cctcggtcaa cacagcactg gttcctcagc tctccacaat ctcacgagcg ctcacacctt
     4261 cccccgttat ggtccttgaa aacattgaac ctgaaattgt atatgcaggc tatgacagct
     4321 caaaaccaga tacagccgaa aatctgctct ccacgctcaa ccgcttagca ggcaaacaga
     4381 tgatccaagt cgtgaagtgg gcaaaggtac ttccaggatt taaaaacttg cctcttgagg
     4441 accaaattac cctaatccgg tattcttgga tgtgtctatc atcatttgcc ttgagctgga
     4501 gatcgtacaa acatacgaac agccaatttc tctattttgc accagaccta gtctttaatg
     4561 aagagaagat gcatcagtct gccatgtatg aactatgcca ggggatgcac caaatcagcc
     4621 ttcagttcgt tcgactgcag ctcacctttg aagaatacac catcatgaaa gttttgctgc
     4681 tactaagcac aattccaaag gatggcctca aaagccaggc tgcatttgaa gaaatgagga
     4741 caaattacat caaagaactg aggaagatgg taactaagtg tcccaacaat tctgggcaga
     4801 gctggcagag gttctaccaa ctgaccaagc tgctggactc catgcatgac ctggtgagcg
     4861 acctgctgga attctgcttc tacaccttcc gagagtccca tgcgctgaag gtagagttcc
     4921 ccgcaatgct ggtggagatc atcagcgacc agctgcccaa ggtggagtcg gggaacgcca
     4981 agccgctcta cttcctccgg aagtgaggta ccgagctcga attcgacact ctaaataagc
     5041 ggatctctta tgtctttacg atttatagtt ttcattatca agtatgccta tattagtata
     5101 tagcatcttt agatgacagt gttcgaagtt tcacgaataa aagataatat tctacttttt
     5161 gctcccaccg cgtttgctag cacgagtgaa caccatccct cgcctgtgag ttgtacccat
     5221 tcgtaaggtg acaagctatt tttcaataaa gaatatcttc cactactgcc atctggcgtc
     5281 ataactgcaa agtacacata tattacgatg ctgttctatt aaatgcttcc tatattatat
     5341 atatagtaat gtcgtgatct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5401 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5521 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5581 gttaatgtca tgataataat ggtttcttag acggatcgct tgcctgtaac ttacacgcgc
     5641 ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg
     5701 ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa
     5761 aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat
     5821 tagacaagaa aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa
     5881 tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata
     5941 cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt
     6001 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt
     6061 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa
     6121 aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6241 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6301 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6361 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6421 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6481 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6541 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6601 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6661 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6721 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6781 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6841 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6901 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6961 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7021 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7081 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7141 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7201 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7261 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7321 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7381 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7741 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7801 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7861 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7921 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7981 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8041 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8101 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8161 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8221 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8281 atgattacga attaattcgc tagagtcgag gccgagtttg tcagaaagca gaccaaacag
     8341 cggttggaat aatagcgaga acagagaaat agcggcaaaa ataatacccg tatcactttt
     8401 gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac gggaagtagg ctcccatgat
     8461 aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag tttgtgtttt ttaaatagta
     8521 cataatggat ttccttacgc gaaatacggg cagacatggc ctgcccggtt attattattt
     8581 ttgacaccag accaactggt aatggtagcg accggcgctc agctgtaatt ccgccgatac
     8641 tgacgggctc caggagtcgt cgccaccaat ccccatatgg aaaccgtcga tattcagcca
     8701 tgtgccttct tccgcgtgca gcagatggcg atggctggtt tccatcagtt gctgttgact
     8761 gtagcggctg atgttgaact ggaagtcgcc gcgccactgg tgtgggccat aattcaattc
     8821 gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg tacggggtat acatgtctga
     8881 caatggcaga tcccagcggt caaaacaggc ggcagtaagg cggtcgggat agttttcttg
     8941 cggccctaat ccgagccagt ttacccgctc tgctacctgc gccagctggc agttcaggcc
     9001 aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca tcaacggtaa tcgccatttg
     9061 accactacca tcaatccggt aggttttccg gctgataaat aaggttttcc cctgatgctg
     9121 ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt gtatctgccg tgcactgcaa
     9181 caacgctgct tcggcctggt aatggcccgc cgccttccag cgttcgaccc aggcgttagg
     9241 gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc agcggtgcac gggtgaactg
     9301 atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc cacatctgtg aaagaaagcc
     9361 tgactggcgg ttaaattgcc aacgcttatt acccagctcg atgcaaaaat ccatttcgct
     9421 ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc gtcacactga ggttttccgc
     9481 cagacgccac tgctgccagg cgctgatgtg cccggcttct gaccatgcgg tcgcgttcgg
     9541 ttgcactacg cgtactgtga gccagagttg cccggcgctc tccggctgcg gtagttcagg
     9601 cagttcaatc aactgtttac cttgtggagc gacatccaga ggcacttcac cgcttgccag
     9661 cggcttacca tccagcgcca ccatccagtg caggagctcg ttatcgctat gacggaacag
     9721 gtattcgctg gtcacttcga tggtttgccc ggataaacgg aactggaaaa actgctgctg
     9781 gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg gcaaagacca gaccgttcat
     9841 acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg ccgtaagccg accacgggtt
     9901 gccgttttca tcatatttaa tcagcgactg atccacccag tcccagacga agccgccctg
     9961 taaacgggga tactgacgaa acgcctgcca gtatttagcg aaaccgccaa gactgttacc
    10021 catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc tctccaggta gcgaaagcca
    10081 ttttttgatg gaccatttcg gcacagccgg gaagggctgg tcttcatcca cgcgcgcgta
    10141 catcgggcaa ataatatcgg tggccgtggt gtcggctccg ccgccttcat actgcaccgg
    10201 gcgggaagga tcgacagatt tgatccagcg atacagcgcg tcgtgattag cgccgtggcc
    10261 tgattcattc cccagcgacc agatgatcac actcgggtga ttacgatcgc gctgcaccat
    10321 tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga tcatcggtca gacgattcat
    10381 tggcaccatg ccgtgggttt caatattggc ttcatccacc acatacaggc cgtagcggtc
    10441 gcacagcgtg taccacagcg gatggttcgg ataatgcgaa cagcgcacgg cgttaaagtt
    10501 gttctgcttc atcagcagga tatcctgcac catcgtctgc tcatccatga cctgaccatg
    10561 cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag
    10621 cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat
    10681 cagcgtgccg tcggcggtgt gcagttcaac caccgcacga tagagattcg ggatttcggc
    10741 gctccacagt ttcgggtttt cgacgttcag acgtagtgtg acgcgatcgg cataaccacc
    10801 acgctcatcg ataatttcac cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc
    10861 ctgccataaa gaaactgtta cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc
    10921 agcctccagt acagcgcggc tgaaatcatc attaaagcga gtggcaacat ggaaatcgct
    10981 gatttgtgta gtcggtttat gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca
    11041 catatcctga tcttccagat aactgccgtc actccagcgc agcaccatca ccgcgaggcg
    11101 gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca gacggcaaac gactgtcctg
    11161 gccgtaaccg acccagcgcc cgttgcacca cagatgaaac gccgagttaa cgccatcaaa
    11221 aataattcgc gtctggcctt cctgtagcca gctttcatca acattaaatg tgagcgagta
    11281 acaacccgtc ggattctccg tgggaacaaa cggcggattg accgtaatgg gataggtcac
    11341 gttggtgtag atgggcgcat cgtaaccgtg catctgccag tttgagggga cgacgacagt
    11401 atcggcctca ggaagatcgc actccagcca gctttccggc accgcttctg gtgccggaaa
    11461 ccaggcaaag cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagtcg
    11581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    11641 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gccaagctcc
    11701 ggatccggtc attattaatt tagtgtgtgt atttgtgttt gcgtgtctat agaagtatag
    11761 taatttatgc tacaaaggac ctaatgtata aggaaagaat atttagagaa aagaagaaaa
    11821 caagagtttt atatacatac agagcacatg cctcgaggtc gaccccggga aaaagaacac
    11881 cctgtaccag acaaaaagaa caccctgtac cagacgtcga caaaaagaac accctgtacc
    11941 agacaaaaag aacaccctgt accagacaaa aagaacaccc tgtaccagac ggatctacta
    12001 gtgcggccgc taagtaagta agacgtcgag ctctaagtaa gtaacggccg ccaccgcggt
    12061 ggagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg
    12121 tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt
    12181 gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
    12241 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
    12301 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
    12361 gctcttattg accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat
    12421 ttcacccaat tgtagatatg ctaactccag caatgagttg atgaatctcg gtgtgtattt
    12481 tatgtcctca gaggacaaca cctgttgtaa tcgttcttcc acacggatcc caattcgccc
    12541 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
    12601 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    12661 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    12721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    12781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    12841 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    12901 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    12961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    13021 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    13081 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    13141 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
    13201 gcatctgtgc ggtatttcac accgcataga tcggcaagtg cac
//
