LOCUS       sequence-310403-        9805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent redox reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9805)
  TITLE     Plasmids expressing redox reporters coupled to fluorescent protein 
            mCherry for lentiviral approaches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti6.2_mCherry_Grx1_roGFP2
FEATURES             Location/Qualifiers
     source          1..9805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1011..1013,1014..1724)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1053..1074)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1314..1333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1661..1682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1728..1781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1782..1791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1794..1803
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1800..2510
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1945..1963)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2213..2232
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2525..3113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2578..2598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2996..3007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3127..3626
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3162..3185)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3559..3578
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3647..4246
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3647..3666)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4143..4163
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4384..4617
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4689..4810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4726..4745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4780..4799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4850..4985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5005..5024)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5006..5024)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5034..5051)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5034..5050)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5043..5065)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5192..5647
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5279..5298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5489..5510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5673..5777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5778..5846,5847..6638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5996..6015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6809..7397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7298..7317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7551..7568
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7685..7706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7721..7726,7727..7744,7745..7751)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7759..7775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7764..7786
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7783..7799
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7783..7799
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7818..7838
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7820..7838
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7866..8092
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8093..8273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8320..8445
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8938..9171
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9356..9400
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9667..9783
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagagatatc atggctcaag
      601 agtttgtgaa ctgcaaaatc cagcctggga aggtggttgt gttcatcaag cccacctgcc
      661 cgtactgcag gagggcccaa gagatcctca gtcaattgcc catcaaacaa gggcttctgg
      721 aatttgtcga tatcacagcc accaaccaca ctaacgagat tcaagattat ttgcaacagc
      781 tcacgggagc aagaacggtg cctcgagtct ttattggtaa agattgtata ggcggatgca
      841 gtgatctagt ctctttgcaa cagagtgggg aactgctgac gcggctaaag cagattggag
      901 ctctgcagac tagtggtggt tcaggtggtg gtggttcagg tggtggtggt tcaggtggag
      961 gaggatcagg aggaggagga tcaggaggag gaggatcagg aggagaattc gtgagcaagg
     1021 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1081 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1141 tgaagttcat ctccaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1201 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1261 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1321 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1381 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1441 actacaactg ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1501 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1561 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcacct
     1621 gctccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1681 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaccgag ggcagaggaa
     1741 gtcttctaac atgcggtgac gtggaggaga atcccggccc tccatggtgg cgcgccacca
     1801 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     1861 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1921 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     1981 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     2041 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     2101 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     2161 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     2221 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     2281 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     2341 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     2401 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     2461 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag gcggccgcgt
     2521 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2581 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2641 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2701 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2761 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2821 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2881 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     2941 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3001 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3061 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     3121 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     3181 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     3241 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     3301 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     3361 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     3421 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     3481 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     3541 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     3601 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     3661 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     3721 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     3781 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     3841 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     3901 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     3961 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     4021 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     4081 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     4141 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     4201 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     4261 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     4321 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4381 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4441 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4501 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4561 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4621 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4681 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4741 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4801 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4861 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4921 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4981 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     5041 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5101 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5161 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5221 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5281 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5341 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5401 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5461 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5521 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5581 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5641 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5701 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5761 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5821 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5881 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5941 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6001 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6061 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6121 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6181 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6241 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6301 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6361 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6421 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6481 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6541 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6601 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6661 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6721 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6781 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6841 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6901 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7321 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7381 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7441 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7501 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7561 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7621 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7681 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7741 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7801 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7861 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7921 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7981 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     8041 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     8101 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8161 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8221 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8281 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8341 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8401 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8461 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8521 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8581 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8641 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8701 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8761 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8821 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8881 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8941 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     9001 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     9061 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     9121 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9181 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9241 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9301 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9361 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9421 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9481 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9541 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     9601 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     9661 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9721 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     9781 tttatcgata agcttgggag ttccg
//
