LOCUS       sequence-310407-        9521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and 
            LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, 
            piggybac-mediated, integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ
  TITLE     Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal
            Labeling and Crosslinking of Proteins on Live Mammalian Cells.
  JOURNAL   Cell Rep. 2020 Jun 23;31(12):107811. doi: 
            10.1016/j.celrep.2020.107811.
  PUBMED    32579937
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-23"; volume: "31(12):107811. doi"; pages: " 
            10.1016/j.celrep.2020.107811"
COMMENT     Sequence Label: pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TA...
FEATURES             Location/Qualifiers
     source          1..9521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   288..350
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2164..2375
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2388..2656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2688..2697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2757..2786
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3192..3212
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    join(4185..4759,4760..4762,4763..4771)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4352..4369)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4579..4598
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4784..5383
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4784..4803)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5280..5300
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5443..5463)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5505..5626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5542..5561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5596..5615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6389..6423
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6914..6930)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6914..6930)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6927..6949)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6938..6954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6962..6968,6969..6986,6987..6992))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7007..7028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7145..7162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7316..7904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7396..7415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8075..8866,8867..8935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8698..8717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8936..9040)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9066..9521)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9203..9224)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9415..9434
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg
      181 tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg
      241 agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata
      301 atcatattgt gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg tgttttatcg
      361 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
      421 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
      481 aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc
      541 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
      601 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
      661 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
      721 tttgagcctg cagacacctg gggggatacg gggaaaaggc ctccacggcc aactagtggc
      781 cttggagctg cagtatttag catgccccac ccatctgcaa ggcattctgg atagtgtcaa
      841 aacagccgga aatcaagtcc gtttatctca aactttagca ttttgggaat aaatgatatt
      901 tgctatgctg gttaaattag attttagtta aatttcctgc tgaagctcta gtacgataag
      961 taacttgacc taagtgtaaa gttgagattt ccttcaggtt tatatagctt gtgcgccgcc
     1021 tgggtacctc gggggacgct ccggcgagca aacgggtctc taaaacctgt aaagcggggt
     1081 tcgacacccc ggtctctcgg acaagtgcgg tttttatcgc tgcagtattt agcatgcccc
     1141 acccatctgc aaggcattct ggatagtgtc aaaacagccg gaaatcaagt ccgtttatct
     1201 caaactttag cattttggga ataaatgata tttgctatgc tggttaaatt agattttagt
     1261 taaatttcct gctgaagctc tagtacgata agtaacttga cctaagtgta aagttgagat
     1321 ttccttcagg tttatatagc ttgtgcgccg cctgggtacc tcgggggacg ctccggcgag
     1381 caaacgggtc tctaaaacct gtaaagcggg gttcgacacc ccggtctctc ggacaagtgc
     1441 ggtttttctt gctgcagtat ttagcatgcc ccacccatct gcaaggcatt ctggatagtg
     1501 tcaaaacagc cggaaatcaa gtccgtttat ctcaaacttt agcattttgg gaataaatga
     1561 tatttgctat gctggttaaa ttagatttta gttaaatttc ctgctgaagc tctagtacga
     1621 taagtaactt gacctaagtg taaagttgag atttccttca ggtttatata gcttgtgcgc
     1681 cgcctgggta cctcggggga cgctccggcg agcaaacggg tctctaaaac ctgtaaagcg
     1741 gggttcgaca ccccggtctc tcggacaagt gcggtttttt tgcctgcagt atttagcatg
     1801 ccccacccat ctgcaaggca ttctggatag tgtcaaaaca gccggaaatc aagtccgttt
     1861 atctcaaact ttagcatttt gggaataaat gatatttgct atgctggtta aattagattt
     1921 tagttaaatt tcctgctgaa gctctagtac gataagtaac ttgacctaag tgtaaagttg
     1981 agatttcctt caggtttata tagcttgtgc gccgcctggg tacctcgggg gacgctccgg
     2041 cgagcaaacg ggtctctaaa acctgtaaag cggggttcga caccccggtc tctcggacaa
     2101 gtgcggtttt tcgtctcact agtgctctag aaaggatctg cgatcgctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
     2401 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     2461 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     2521 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     2581 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     2641 tgcgccgtta cagatccaag ctgtgaccgg cgcctactct agagctagcc accatggcat
     2701 tgcccgtgac cgccctgctg ctgccactgg ccttgttgct ccacgccgcg cggccagaac
     2761 agaagctgat cagcgaggag gatctgatgg ctgatgtgca actcgtagag tccggtggcg
     2821 gactcgtcca ggcgggaggc tccctgcgcc tgagttgcgc tgcgtccgga agaactatta
     2881 gcatggccgc catgagttgg ttcagacagg cgcctggaaa ggagagggag ttcgtggcag
     2941 gcataagtcg ctccgcaggg tcagccgtcc atgccgactc cgtaaaggga cgctttacca
     3001 tctccagaga taacacaaaa aacacgctct atctccaaat gaattctttg aaggccgagg
     3061 acacagccgt gtactactgc gccgttagga ccagcggatt ttttggaagc attcctagga
     3121 cagggacagc ctttgattac tggggccagg gaacgcaggt taccgtttcc atcctggact
     3181 acagcttcac agagaacctg tacttccaag gtggcgctgg gcgcgacatt cccccaccgc
     3241 agattgagga ggcctgtgag ctgcctgagt gccaggtgga tgcaggcaat aaggtctgca
     3301 actaacagtg taataatcac gcatgtggct gggatggtgg cgactgctcc ctcaacttca
     3361 atgacccctg gaagaactgc acgcagtctc tacagtgctg gaagtatttt agcgacggcc
     3421 actgtgacag ccagtgcaac tcggccggct gcctctttga tggcttcgac tgccagctca
     3481 ccgagggaca gtgcaacccc ctgtatgacc agtactgcaa ggaccacttc agtgatggcc
     3541 actgcgacca gggctgtaac agtgccgaat gtgagtggga tggcctagac tgtgctgagc
     3601 atgtacccga gcggctggca gccggcaccc tggtgctggt ggtgctgctt ccacccgacc
     3661 agctacggaa caactccttc cactttctgc gggagctcag ccacgtgctg cacaccaacg
     3721 tggtcttcaa gcgtgatgcg caaggccagc agatgatctt cccgtactat ggccacgagg
     3781 aagagctgcg caagcaccca atcaagcgct ctacagtggg ttgggccacc tcttcactgc
     3841 ttcctggtac cagtggtggg cgccagcgca gggagctgga ccccatggac atccgtggct
     3901 ccattgtcta cctggagatc gacaaccggc aatgtgtgca gtcatcctcg cagtgcttcc
     3961 agagtgccac cgatgtggct gccttcctag gtgctcttgc gtcacttggc agcctctaga
     4021 ttccttacaa gattgaggcc gtgaagagtg agccggtgga gcctccgctg ccctcgcagc
     4081 tgcacctcat gtacgtggca gcggccgcct tcgtgctcct gttctttgtg ggctgtgggg
     4141 tgctgctgtc ccgcaagcgc cggcggtgag gatccgcggc cgcgcccctc tccctccccc
     4201 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     4261 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     4321 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     4381 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     4441 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     4501 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     4561 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4621 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4681 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     4741 tgaaaaacac gatgataata tggccacaac catggcgtcc ggaatgaccg agtacaagcc
     4801 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     4861 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4921 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4981 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5041 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5101 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5161 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5221 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5281 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5341 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     5401 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5461 atgtggatac gctgctttaa tgcctttgta tcatgcgtta actaaacttg tttattgcag
     5521 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5581 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt
     5641 gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc cgggggacaa
     5701 gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc
     5761 tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca
     5821 gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg
     5881 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     5941 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     6001 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     6061 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc
     6121 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
     6181 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga
     6241 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca
     6301 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt
     6361 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta
     6421 gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc
     6481 ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt
     6541 gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa
     6601 cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg
     6661 ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg
     6721 gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc
     6781 ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat
     6841 cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct
     6901 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6961 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7021 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7081 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7141 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7201 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7261 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7321 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7381 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7441 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7501 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7561 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7621 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7681 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7741 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7801 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7861 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7921 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7981 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8041 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8101 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8161 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8221 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8281 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8341 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8401 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8461 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8521 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8581 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8641 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8701 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8761 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8821 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8881 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8941 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9001 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9061 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     9121 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     9181 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     9241 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     9301 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     9361 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     9421 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     9481 caccacaccc gccgcgctta atgcgccgct acagggcgcg t
//
