LOCUS       sequence-31042-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-COR-Y1699C
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             109..138
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             154..183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    202..226
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctggttt agtgaccgtc agatccgcta gcgattacgc caagctcgaa attaaccctc
       61 actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgccatgga gcagaaactc
      121 atctctgaag aggatctgag cccgggcgga tccgagcaga aactcatctc tgaagaggat
      181 ctgctgcagg aattcgatat cacaagtttg tacaaaaaag caggctccga attcgccctt
      241 atgacgcagc gagcattgta ccttgctgtc tatgacctca gcaagggaca ggctgaagtt
      301 gatgccatga agccttggct cttcaatata aaggctcgcg cttcttcttc ccctgtgatt
      361 ctcgttggca cacatttgga tgtttctgat gagaagcaac gcaaagcctg catgagtaaa
      421 atcaccaagg aactcctgaa taagcgaggg ttccctgcca tacgagatta ccactttgtg
      481 aatgccaccg aggaatctga tgctttggca aaacttcgga aaaccatcat aaacgagagc
      541 cttaatttca agatccgaga tcagcttgtt gttggacagc tgattccaga ctgctatgta
      601 gaacttgaaa aaatcatttt atcggagcgt aaaaatgtgc caattgaatt tcccgtaatt
      661 gaccggaaac gattattaca actagtgaga gaaaatcagc tgcagttaga tgaaaatgag
      721 cttcctcacg cagttcactt tctaaatgaa tcaggagtcc ttcttcattt tcaagaccca
      781 gcactgcagt taagtgactt gtactttgtg gaacccaagt ggctttgtaa aatcatggca
      841 cagattttga cagtgaaag
//
