LOCUS       sequence-310437-       10768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for sgRNA, NGFR marker, editing detected with 
            GFP, used with 155280.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10768)
  TITLE     Lenitviral sgRNA and Cas9 constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lenti sgRNA NGFR GFP out of frame
FEATURES             Location/Qualifiers
     source          1..10768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1894..2142
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1894..1914
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2065..2084
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    4168..4285
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4354..4565
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4585..4594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4619..4621,4622..5335)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4619..4621,4622..5332)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4661..4682)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4922..4941)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5269..5290
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5350..5902
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5494..5511)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5721..5740
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6802..7390
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6855..6875)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7273..7284)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7462..7695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7773..7894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7810..7829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7864..7883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7934..8069
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8089..8108)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8090..8108)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8118..8135)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8118..8134)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8127..8149)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8276..8731
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8363..8382)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8573..8594
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8757..8861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8862..8930,8931..9722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9080..9099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9893..10481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10382..10401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10635..10652
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc agagagggcc tatttcccat gattccttca
     1921 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
     1981 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     2041 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     2101 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggagacgg ttgtaaatga
     2161 gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa tcaaccaaaa
     2221 tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt tttgcaaact
     2281 tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt tgttttcttc
     2341 acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg cttcaatcat
     2401 tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag cattgtcttt
     2461 aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg aaaatcctgt
     2521 tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt tcatgatttc
     2581 ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc cagctgcttt
     2641 ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta atgcaccaat
     2701 atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa cttcgaatgc
     2761 ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta ttcctacatt
     2821 tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac aaactgttgc
     2881 aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg tagcatcttg
     2941 ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta tatattcatt
     3001 tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg aacagattca
     3061 ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg aacagcaaat
     3121 catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt atacattcca
     3181 tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa gggtgttgct
     3241 ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt aaaattcaag
     3301 tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa atagtctact
     3361 aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat agaagcttgt
     3421 attccatctt tatctctagc tgagtcatca attacatatc cataactttc ttcataagca
     3481 aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat tcatttaaat
     3541 ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct atcacccaaa
     3601 tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt taagcgtttt
     3661 agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc taatcttaca
     3721 aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc attcataata
     3781 ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc aggatcaaat
     3841 tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg ctcttcaacc
     3901 tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt tcctgttcca
     3961 ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt tttgtacgtc
     4021 tctgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     4081 aactttgctg gaaacagcaa agtggcaccg agtcggtgct ttttttgaat tctagatctt
     4141 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4201 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4261 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     4321 gcgccggctc gagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     4381 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     4441 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     4501 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     4561 cacaggtgtc gtgacgcggg atccgccacc atggggaaga tgggcgggag tcttcagggt
     4621 tagtaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4681 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4741 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4801 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     4861 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4921 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     4981 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5041 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5101 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5161 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5221 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5281 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtagcgccc
     5341 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5401 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5461 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5521 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5581 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5641 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5701 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5761 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5821 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5881 tttgaaaaac acgatgataa tatggccaca accatggacg ggccgcgcct gctgctgttg
     5941 ctgcttctgg gggtgtccct tggaggtgcc aaggaggcat gccccacagg cctgtacaca
     6001 cacagcggtg agtgctgcaa agcctgcaac ctgggcgagg gtgtggccca gccttgtgga
     6061 gccaaccaga ccgtgtgtga gccctgcctg gacagcgtga cgttctccga cgtggtgagc
     6121 gcgaccgagc cgtgcaagcc gtgcaccgag tgcgtggggc tccagagcat gtcggcgccg
     6181 tgcgtggagg ccgacgacgc cgtgtgccgc tgcgcctacg gctactacca ggatgagacc
     6241 actgggcgct gcgaggcgtg ccgcgtgtgc gaggcgggct cgggcctcgt gttctcctgc
     6301 caggacaagc agaacaccgt gtgcgaggag tgccccgacg gcacgtattc cgacgaggcc
     6361 aaccacgtgg acccgtgcct gccctgcacc gtgtgcgagg acaccgagcg ccagctccgc
     6421 gagtgcacac gctgggccga cgccgagtgc gaggagatcc ctggccgttg gattacacgg
     6481 tccacacccc cagagggctc ggacagcaca gcccccagca cccaggagcc tgaggcacct
     6541 ccagaacaag acctcatagc cagcacggtg gcaggtgtgg tgaccacagt gatgggcagc
     6601 tcccagcccg tggtgacccg aggcaccacc gacaacctca tccctgtcta ttgctccatc
     6661 ctggctgctg tggttgtggg ccttgtggcc tacatagcct tcaagaggtg gaacagtcat
     6721 cgatatcctc gaggtcaccg cggtctagag tcgacctgca gccaagctta tcgataagta
     6781 caagtagcgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7201 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7321 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7381 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     7501 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7681 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     7741 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7921 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7981 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8041 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8101 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8161 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8221 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8281 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8341 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8401 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8461 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8521 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8581 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8641 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8701 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     8761 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8821 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8881 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8941 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9001 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9061 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9121 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9181 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9241 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9301 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9361 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9421 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9481 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9541 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9601 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9661 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9721 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9781 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9841 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9961 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10021 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10501 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10561 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10621 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10681 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10741 ggaaagcggg cagtgagcgc aacgcaat
//
