LOCUS       sequence-310439-        9350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral sgRNA cloning with CFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9350)
  TITLE     Lenitviral sgRNA and Cas9 constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lenti sgRNA CFP
FEATURES             Location/Qualifiers
     source          1..9350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1894..2142
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1894..1914
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2065..2084
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    4168..4285
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4354..4565
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(4649..4651,4652..4654,4655..5368)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4694..4715)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4955..4974)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5302..5323
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5384..5972
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5437..5457)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5855..5866)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6044..6277
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6355..6476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6392..6411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6446..6465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6516..6651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6671..6690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6672..6690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6700..6717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6700..6716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6709..6731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6858..7313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6945..6964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7155..7176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7339..7443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7444..7512,7513..8304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7662..7681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8475..9063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8964..8983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9217..9234
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc agagagggcc tatttcccat gattccttca
     1921 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
     1981 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     2041 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     2101 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggagacgg ttgtaaatga
     2161 gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa tcaaccaaaa
     2221 tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt tttgcaaact
     2281 tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt tgttttcttc
     2341 acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg cttcaatcat
     2401 tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag cattgtcttt
     2461 aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg aaaatcctgt
     2521 tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt tcatgatttc
     2581 ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc cagctgcttt
     2641 ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta atgcaccaat
     2701 atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa cttcgaatgc
     2761 ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta ttcctacatt
     2821 tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac aaactgttgc
     2881 aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg tagcatcttg
     2941 ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta tatattcatt
     3001 tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg aacagattca
     3061 ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg aacagcaaat
     3121 catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt atacattcca
     3181 tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa gggtgttgct
     3241 ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt aaaattcaag
     3301 tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa atagtctact
     3361 aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat agaagcttgt
     3421 attccatctt tatctctagc tgagtcatca attacatatc cataactttc ttcataagca
     3481 aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat tcatttaaat
     3541 ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct atcacccaaa
     3601 tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt taagcgtttt
     3661 agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc taatcttaca
     3721 aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc attcataata
     3781 ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc aggatcaaat
     3841 tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg ctcttcaacc
     3901 tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt tcctgttcca
     3961 ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt tttgtacgtc
     4021 tctgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     4081 aactttgctg gaaacagcaa agtggcaccg agtcggtgct ttttttgaat tctagatctt
     4141 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4201 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4261 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     4321 gcgccggctc gagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     4381 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     4441 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     4501 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     4561 cacaggtgtc gtgacgcggg atccactagt aacggccgcc agtgtgctgg aattctgcag
     4621 atatccatca cactggcggc cgctcgagat ggtgagcaag ggcgaggagc tgttcaccgg
     4681 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaggt tcagcgtgtc
     4741 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
     4861 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4921 aggctacgtc caggagcgta ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt
     5101 ctatatcacc gccgacaagc agaagaacgg catcaaggcc cacttcaaga tccgccacaa
     5161 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5221 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5281 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5341 tctcggcatg gacgagctgt acaagtagac gcgttaagtc gacaatcaac ctctggatta
     5401 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5461 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5521 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5581 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5641 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5701 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5761 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     5821 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5881 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5941 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     6001 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6061 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6181 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6241 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     6301 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6481 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6601 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6661 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6721 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6781 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6841 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6901 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6961 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7021 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7081 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7141 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7201 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7261 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7321 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7681 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7981 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9241 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9301 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
//
