LOCUS       sequence-310440-       11242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of humnan CIT shRNA, RFP i670 expression, 
            based on Addgene 12247.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11242)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lentiviral human Citron shRNA 1 RFP i670
FEATURES             Location/Qualifiers
     source          1..11242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2146..3324
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2377..3315
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3272..3292
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3372..3489
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3522..3531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3528..4475
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    4495..5083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4548..4568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4966..4977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5251..5269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5293..5311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5335..5353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5377..5395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5419..5437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5461..5479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5508..5722
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5643..5662
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5804..5837)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5857..6037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6062..6080)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6063..6080)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6370..6389)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6603..6622)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6722..6744
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6782..6800)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6868..6972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6973..7041,7042..7833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7191..7210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8004..8592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8493..8512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8746..8763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8833..8853)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8838..9167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9018..9153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10301..10366
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10496..10516
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10941..11075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10978..10997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11032..11051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgcgat gtcgacgata agctttgcaa agatggataa agttttaaac agagaggaat
     2101 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2161 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2221 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2281 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2341 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     2401 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     2461 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     2521 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     2581 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     2641 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     2701 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     2761 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     2821 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     2881 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     2941 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3001 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3061 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3121 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3181 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3241 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3301 tttcttccat ttcaggtgtc gtgaggaatt tcgacattta aatttaatta atctcgacgg
     3361 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3421 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3481 caaaatttta tcgatcacga gactagcctc gaggtttaaa cgccaccatg gtagcaggtc
     3541 atgcctctgg cagccccgca ttcgggaccg cctctcattc gaattgcgaa catgaagaga
     3601 tccacctcgc cggctcgatc cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc
     3661 atcgcgtcat ccaggccagc gccaacgccg cggaatttct gaatctcgga agcgtactcg
     3721 gcgttccgct cgccgagatc gacggcgatc tgttgatcaa gatcctgccg catctcgatc
     3781 ccaccgccga aggcatgccg gtcgcggtgc gctgccggat cggcaatccc tctacggagt
     3841 actgcggtct gatgcatcgg cctccggaag gcgggctgat catcgaactc gaacgtgccg
     3901 gcccgtcgat cgatctgtca ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg
     3961 gttcactgcg cgcgctgtgc gatgacaccg tgctgctgtt tcagcagtgc accggctacg
     4021 accgggtgat ggtgtatcgt ttcgatgagc aaggccacgg cctggtattc tccgagtgcc
     4081 atgtgcctgg gctcgaatcc tatttcggca accgctatcc gtcgtcgact gtcccgcaga
     4141 tggcgcggca gctgtacgtg cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc
     4201 cggtgccgct ggagccgcgg ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct
     4261 gcttcctgcg ctcgatgtcg ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg
     4321 ccaccctggc ggtgtcgctg gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc
     4381 attatctgcc gcgcttcatc cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa
     4441 ggatcgcgac gcggatcacc gcgcttgaga gctaacgggg actagtcata tgataatcaa
     4501 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4561 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4621 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4681 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4741 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4801 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4861 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     4921 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4981 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5041 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggta cgtatggcca
     5101 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5161 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc
     5221 atgctagtgg atccccctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     5281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     5341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     5401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     5461 tccctatcag tgatagagaa aagtgaaagt cggggctgca ggaattcgaa cgctgacgtc
     5521 atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg
     5581 tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc aatatttgca
     5641 tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct
     5701 tataagttct gtatgagacc acgcgcccat ggaaggcact atttctcaat tcaagagatt
     5761 gagaaatagt gccttccatt ttttacgata ccgtcgcatg ggaataactt cgtatagcat
     5821 acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5881 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5941 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6001 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     6061 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     6121 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     6181 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     6241 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     6301 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     6361 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     6421 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     6481 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     6541 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     6601 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     6661 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6721 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     6781 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6841 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6901 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6961 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7021 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7081 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7141 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7201 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7261 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7321 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7381 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7441 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7501 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7561 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7621 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7681 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7741 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7801 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7861 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7921 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7981 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8041 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8101 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8161 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8221 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8281 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8341 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8401 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8461 ggaacaggag agcgcacgaa ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8521 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8581 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8641 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8701 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8761 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8821 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8881 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     9001 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9061 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     9121 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     9181 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     9241 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     9301 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     9361 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     9421 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     9481 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     9541 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     9601 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     9661 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     9721 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     9781 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     9841 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     9901 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     9961 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
    10021 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
    10081 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
    10141 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
    10201 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
    10261 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
    10321 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
    10381 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
    10441 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
    10501 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
    10561 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
    10621 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
    10681 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
    10741 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
    10801 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
    10861 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    10921 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    10981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11041 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    11101 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    11161 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    11221 tatgtactac aaacttagta gt
//
