LOCUS       sequence-31047-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-WD40
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..58
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..128
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             144..173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    192..216
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgattacgc caagctcgaa attaaccctc actaaaggga
       61 acaaaagctg gagctccacc gcggtggcgg ccgccatgga gcagaaactc atctctgaag
      121 aggatctgag cccgggcgga tccgagcaga aactcatctc tgaagaggat ctgctgcagg
      181 aattcgatat cacaagtttg tacaaaaaag caggctccga attcgccctt gttgctacac
      241 atcacaacag caggaatgca agcatttggc tgggctgtgg gcacaccgac agaggacagc
      301 tctcatttct tgacttaaat actgaaggat acacttctga ggaagttgct gatagtagaa
      361 tattgtgctt agccttggtg catcttcctg ttgaaaagga aagctggatt gtgtctggga
      421 cacagtctgg tactctcctg gtcatcaata ccgaagatgg gaaaaagaga cataccctag
      481 aaaagatgac tgattctgtc acttgtttgt attgcaattc cttttccaag caaagcaaac
      541 aaaaaaattt tcttttggtt ggaaccgctg atggcaagtt agcaattttt gaagataaga
      601 ctgttaagct taaaggagct gctcctttga agatactaaa tataggaaat gtcagtactc
      661 cattgatgtg tttgagtgaa tccacaaatt caacggaaag aaatgtaatg tggggaggat
      721 gtggcacaaa gattttctcc ttttctaatg atttcaccat tcagaaactc attgagacaa
      781 gaacaagcca actgttttct tatgcagctt tcagtgattc caacatcata acagtggtgg
      841 tagacactgc tctctatatt gctaagcaaa atagccctgt tgtggaagtg tgggataaga
      901 aaactgaaaa ctctgtggac tatagact
//
