LOCUS       sequence-310865-        1524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of inserted PLY variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Shah NR, Voisin TB, Parsons ES, Boyd CM, Hoogenboom BW, Bubeck D
  TITLE     Structural basis for tuning activity and membrane specificity of 
            bacterial cytolysins.
  JOURNAL   Nat Commun. 2020 Nov 16;11(1):5818. doi: 10.1038/s41467-020-19482-6.
  PUBMED    33199689
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-16"; volume: "11(1):5818. doi"; pages: " 
            10.1038/s41467-020-19482-6"
COMMENT     Sequence Label: pTricHis-PLY
FEATURES             Location/Qualifiers
     source          1..1524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatcgat ggggatccat ggcaaataaa
      121 gcggtaaatg actttattct tgcaatgaat tacgataaga agaaacttct gacccatcag
      181 ggcgaatcga ttgaaaatcg tttcatcaaa gagggtaatc agcttcccga tgagtttgta
      241 gtcatcgaac gtaagaagcg tagtctctcg actaatacca gtgatatttc tgtgacggcc
      301 actaacgaca gccgcctgta tccgggagcc ctgttggtag tggatgagac cttgctggag
      361 aataatccta cgttacttgc cgtagatcgt gccccgatga cgtattcaat tgatctgcca
      421 ggtttggctt catccgattc gttcttacaa gtggaagacc cgagcaattc ttctgtacgc
      481 ggtgcggtaa acgatctgct ggcgaagtgg catcaagatt atggtcaggt taataatgtt
      541 ccagcccgca tgcagtatga aaagatcacg gctcacagta tggaacaact gaaggtgaag
      601 tttggatcag actttgaaaa gaccggaaat tcgctggata ttgattttaa ctcagtgcat
      661 tccggagaga agcagattca gattgttaat ttcaagcaga tttattatac cgtgagcgtt
      721 gacgcagtga agaatccggg tgatgtgttt caagatacag tcactgttga ggatttaaaa
      781 cagcgtggta tttccgcaga gcgtcctctc gtttatatta gcagtgtggc gtatggtcgc
      841 caagtatatt tgaagttaga aacaacgtcc aagtctgatg aagtagaggc ggcatttgaa
      901 gcgctcatca aaggggtcaa ggtggcccca cagaccgagt ggaagcagat tttggacaat
      961 acggaagtga aggcggttat tctgggtggc gacccttcgt ccggggcccg cgtcgtaaca
     1021 ggcaaggtgg atatggtgga agacttgatt caggaaggat cccgctttac cgcggatcat
     1081 cctggtcttc ccatttcgta tactaccagt tttcttcgtg acaatgtggt ggccaccttt
     1141 caaaactcta ccgactatgt ggagacgaag gtcaccgcct accgtaacgg tgatctcctt
     1201 ctggatcatt cgggcgcata tgtagcgcaa tattatatta cttgggatga actgagttat
     1261 gatcatcaag gcaaggaagt acttactccc aaggcatggg accgtaatgg ccaggatctt
     1321 acggcgcact ttactaccag cattcctctc aaaggtaatg tgcgtaatct tagtgttaaa
     1381 attcgtgagt gtaccggact tgcctgggaa tggtggcgta ccgtttatga gaaaaccgat
     1441 ctcccactgg tccgtaagcg tacaattagt atttggggaa ctacactgta tcctcaggta
     1501 gaggataagg ttgaaaatga ctaa
//
