LOCUS       sequence-310903-        9663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses IntCCCre in mammalian cells in split intein-mediated 
            split-Cre recombinase applications.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Khoo ATT, Kim PJ, Kim HM, Je HS
  TITLE     Neural circuit analysis using a novel intersectional split 
            intein-mediated split-Cre recombinase system.
  JOURNAL   Mol Brain. 2020 Jul 2;13(1):101. doi: 10.1186/s13041-020-00640-2.
  PUBMED    32616061
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2020-07-2"; volume: "13(1):101. doi"; pages: " 
            10.1186/s13041-020-00640-2"
COMMENT     Sequence Label: pCDH-CAG-IntCCCre-T2A-CopGFP
FEATURES             Location/Qualifiers
     source          1..9663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     537..556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(775..879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     947..965
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1003..1025)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1125..1144
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1338..1360
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1352..1369
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1353..1369
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1384..1610
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1611..1791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1838..1963
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2456..2689
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2874..2918
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3185..3301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3357..3379
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3395..3411
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        3441..3820
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3823..4098
                     /label=chicken beta-actin promoter
     intron          4099..5107
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5031..5053
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      5170..5179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5179..5202
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6229..6252
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6262..6315
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6346..7008
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    7115..7703
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7168..7188)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7586..7597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7777..8010
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8082..8203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8119..8138)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8173..8192
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8243..8378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8416..8432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8416..8432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8429..8451)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8440..8456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8464..8470,8471..8488,8489..8494))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8509..8530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8647..8664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8818..9406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9577..9663,1..705,706..774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
       61 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      901 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      961 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     1021 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     1081 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     1141 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     1201 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1261 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1321 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgac
     1381 gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     1441 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     1501 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     1561 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     1621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     1681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1801 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1861 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1921 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1981 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2041 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2101 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     2161 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     2221 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2281 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
     2341 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     2401 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     2461 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     2521 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     2581 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2641 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2701 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2761 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2821 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2881 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2941 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3001 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3061 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     3121 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     3181 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     3241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
     3301 tatcgatgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     3361 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     3421 aggcgccgga attcgatgtc gacattgatt attgactagt tattaatagt aatcaattac
     3481 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3541 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3601 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac
     3661 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3721 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3781 ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt
     3841 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     3901 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg
     3961 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
     4021 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     4081 cgaagcgcgc ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc
     4141 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     4201 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt
     4261 ctgtggctgc gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg
     4321 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc
     4381 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg
     4441 ggagcgcggc cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg
     4501 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta
     4561 acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     4621 tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     4681 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     4741 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     4801 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg
     4861 cgccgccgca ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa
     4921 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc
     4981 ggggctgccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     5041 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     5101 cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt
     5161 cttaattaag ccaccatgga ttataaagac gatgacgata aaatggttaa agttatcggt
     5221 cgtcgttccc tcggagtgca aagaatattt gatattggtc ttcgccaaga ccataatttt
     5281 ctgctagcca atggggcgat cgcggccaat tgcttcaata aggaggacgg atccaaccgg
     5341 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     5401 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     5461 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatacga
     5521 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     5581 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     5641 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     5701 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     5761 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     5821 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     5881 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     5941 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
     6001 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
     6061 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     6121 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     6181 ggcgatgaat tctgcagata tccatcacac tggcggccgc tcgagatgga ctacaaggac
     6241 gacgatgata agggcaccgg tgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6301 gagaatcccg gccctcccgg gatggagagc gacgagagcg gcctgcccgc catggagatc
     6361 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     6421 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     6481 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     6541 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     6601 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     6661 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     6721 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     6781 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     6841 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     6901 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     6961 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     7021 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccct gtacaagtaa
     7081 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     7141 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7201 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7261 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7321 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7381 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7441 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7501 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7561 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7621 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     7681 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     7741 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7801 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7861 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7921 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7981 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8041 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8101 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8161 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8221 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8281 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8341 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     8401 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8461 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8521 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8581 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8641 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8701 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8761 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8821 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8881 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8941 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9001 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9061 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9121 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9181 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9241 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9301 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9361 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9421 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9481 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9541 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9601 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9661 gat
//
