LOCUS       sequence-310946-        4572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ethanol dependent transcrition factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4572)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 AlcR (GB1550)
FEATURES             Location/Qualifiers
     source          1..4572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2509..2566
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2590..2607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2761..3349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2841..2860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3532..3626)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3647..4306)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4221..4240
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     4468..4486
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4490..4533)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4536..4557
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggcagat acgcgccgac gccagaatca tagctgcgat ccctgtcgca agggcaagcg
       61 acgctgtgat gccccggaaa atagaaacga ggccaatgaa aacggctggg tttcgtgttc
      121 aaattgcaag cgttggaaca aggattgtac cttcaattgg ctctcatccc aacgctccaa
      181 ggcaaaaggg gctgcaccta gagcgagaac aaagaaagcc aggaccgcaa caaccaccag
      241 tgaaccatca acttcagctg caacaatccc tacaccggaa agtgacaatc acgatgcgcc
      301 tccagtcata aactctcacg acgcgctccc gagctggact caggggctac tctcccaccc
      361 cggcgacctt ttcgatttca gccactctgc tattcccgca aatgcagaag atgcggccaa
      421 cgtgcagtca gacgcacctt ttccgtggga tctagccatc cccggtgatt tcagcatggg
      481 ccaacagctc gagaaacctc tcagtccgct cagttttcaa gcagtccttc ttccgcccca
      541 tagcccgaac acggatgacc tcattcgcga gctggaagag cagactacgg atccggactc
      601 ggttaccgat actaatagtg tacaacaggt cgctcaagat ggatcgctat ggtctgatcg
      661 gcagtcgccg ctactgcctg agaacagtct gtgcatggcc tcagacagca cagcacggcg
      721 atatgcccgt tccacaatga cgaagaatct gatgcgaatc taccacgata gtatggagaa
      781 tgcactgtcc tgctggctga cagagcacaa ttgtccatac tccgaccaga tcagctacct
      841 gccgcccaag cagcgggcgg aatggggccc gaactggtca aacaggatgt gcatccgggt
      901 gtgccggcta gatcgcgtat ctacctcatt acgcgggcgc gccctgagtg cggaagagga
      961 caaagccgca gcccgagccc tgcatctggc gatcgtagct tttgcgtcgc aatggacgca
     1021 gcatgcgcag aggggggctg ggctaaatgt tcctgcagac atagccgccg atgagaggtc
     1081 catccggagg aacgcctgga atgaagcacg ccatgccttg cagcacacga cagggattcc
     1141 atcattccgg gttatatttg cgaatatcat cttttctctc acgcagagtg tgctggatga
     1201 tgatgagcag cacggtatgg gtgcacgtct agacaagcta ctcgaaaatg acggtgcgcc
     1261 cgtgttcctg gaaaccgcga accgtcagct ttatacattc cgacataagt ttgcacgaat
     1321 gcaacgccgc ggtaaggctt tcaacaggct cccgggagga tctgtcgcat cgacattcgc
     1381 cggtattttc gagacaccga cgccgtcgtc tgaaagccca cagcttgacc cggttgtggc
     1441 cagtgaggag catcgcagta cattaagcct tatgttctgg ctagggatca tgttcgatac
     1501 actaagcgct gcaatgtacc agcgaccact cgtggtgtca gatgaggata gccagatatc
     1561 atcggcatct ccaccaaggc gcggcgctga aacgccgatc aacctagact gctgggagcc
     1621 cccgagacag gtcccgagca atcaagaaaa gagcgacgta tggggcgacc tcttcctccg
     1681 cacctcggac tctctcccag atcacgaatc ccacacacaa atctctcagc cagcggctcg
     1741 atggccctgc acctacgaac aggccgccgc cgctctctcc tctgcaacgc ccgtcaaagt
     1801 cctcctctac cgccgcgtca cgcagctcca aaccctcctc tatcgcggcg ccagccctgc
     1861 ccgccttgaa gcggccatcc agagaacgct ctacgtttat aatcactgga cagcgaagta
     1921 ccaaccattt atgcaggact gcgttgctaa ccacgagctc ctcccttcgc gcatccagtc
     1981 ttggtacgtc attctagacg gtcactggca tctagccgcg atgttgctag cggacgtttt
     2041 ggagagcatc gaccgcgatt cgtactctga tatcaaccac atcgaccttg taacaaagct
     2101 aaggctcgat aatgcactag cagttagtgc ccttgcgcgc tcttcactcc gaggccagga
     2161 gctggacccg ggcaaagcat ctccgatgta tcgccatttc catgattctc tgaccgaggt
     2221 ggcattcctg gtagaaccgt ggaccgtcgt tcttattcac tcgtttgcca aagctgcgta
     2281 tatcttgctg gactgtttag atctggacgg ccaaggaaat gcactagcgg ggtacctgca
     2341 gctgcggcaa aattgcaact actgcattcg ggcgctgcaa tttctgggca ggaagtcgga
     2401 tatggcggcg ctggttgcga aggatttaga gagaggtttg aatgggaaag ttgacagctt
     2461 tttgtaggct ttgagaccac tcatcgcgac tagtagcggc cgctgcagtc cggcaaaaaa
     2521 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     2581 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2761 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3181 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3481 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc
     3541 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     3601 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     3661 gccactcgtc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     3721 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     3781 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     3841 aactggtgaa actcacccag ggattggctg atacgaaaaa catattctca ataaaccctt
     3901 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     3961 actgccggaa atcgtcgtgg tattcactcc agagtgatga aaacgtttca gtttgctcat
     4021 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     4081 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     4141 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     4201 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     4261 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     4321 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     4381 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc
     4441 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     4501 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagcga
     4561 tgagtggtct cg
//
