LOCUS       sequence-310976-        9211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the regulated expression of the PhiC31 phage integrase gene 
            driven by a synthetic promoter consisting of the LacI operator fused
            to the minimal 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..51
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    84..103
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    136..155
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    188..207
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    240..259
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    292..311
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        385..431
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             506..2320
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             2321..2341
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2368..2620
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2655..2673)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2861..2885
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4186..4815
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5249..6316
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6382..6576
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6812..6834)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6920..7060
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6975..6994
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7075..7092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7326..7345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7922..8737)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7922..8737)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8645..8664
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9170..9194
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagttgcga agatcgatcc actagtcttt caattgtgag cgctcacaat tctttctctt
       61 ccctttcttc tttctagtct ttcaattgtg agcgctcaca attctttctc ttccctttct
      121 tctttctagt ctttcaattg tgagcgctca caattctttc tcttcccttt cttctttcta
      181 gtctttcaat tgtgagcgct cacaattctt tctcttccct ttcttctttc tagtctttca
      241 attgtgagcg ctcacaattc tttctcttcc ctttcttctt tcttgtcttt caattgtgag
      301 cgctcacaat tctttctctt ccctttcttc tttctagctc caccgcggtg gcggccggcc
      361 gctctagtgg atcgattccc cttcgcaaga cccttcctct atataaggaa gttcatttca
      421 tttggagagg actccggtat ttttacaaca attaccacaa caaaacaaac aacaaacaac
      481 attacaattt actattctag tcgaaatgga cacgtatgcc ggcgcttatg atagacaatc
      541 aagagaaaga gagaactctt ctgccgcctc tccagctaca cagagatctg ccaacgagga
      601 taaagctgct gatttacaaa gagaggtaga gagagatggg ggtaggttta gatttgtggg
      661 tcatttctct gaggctcctg gtacatcagc ctttggtact gctgaacggc ctgaatttga
      721 aagaatatta aatgagtgtc gggccggtcg actaaatatg ataatagttt atgatgtatc
      781 taggttcagt agactcaaag ttatggatgc aattccgatc gtgagcgagc tacttgcact
      841 cggtgttact atcgtaagta cacaagaagg tgtattcagg cagggtaatg tgatggacct
      901 tatacacctt attatgcgac tcgacgctag ccataaagag tcatcactga agagtgctaa
      961 aatcttggac accaagaacc tgcaaaggga gcttggaggc tatgtaggag gaaaagctcc
     1021 gtatggtttc gaactcgtga gtgagacaaa agagattacc agaaatggta ggatggtgaa
     1081 cgtggttatt aataagctag ctcatagcac aacccctctc acagggcctt ttgagttcga
     1141 acctgacgtt attagatggt ggtggagaga gatcaaaact cacaaacatc ttccatttaa
     1201 gccaggtagc caggctgcta tccaccctgg atctatcact gggctttgta aaaggatgga
     1261 tgctgacgca gtgccaactc gaggggaaac aatcggtaag aagactgcat cttccgcttg
     1321 ggatcccgct acagttatga gaatccttcg ggatccgcgt attgctggat tcgccgcaga
     1381 agtcatttat aagaaaaaac ctgatggtac tcctactaca aaaatcgaag gttataggat
     1441 ccaaagggat cctattactt tgcgtccagt tgaattagac tgtggaccga tcatcgaacc
     1501 tgccgagtgg tacgagttgc aggcctggct tgatggtaga gggcgtggaa agggtttatc
     1561 tcggggacag gcaattttgt cagcaatgga taagctttat tgtgagtgtg gggctgttat
     1621 gacatcaaaa cgtggagaag agtcaatcaa ggattcttac aggtgcagac gtcgtaaagt
     1681 ggtggaccct tctgctccag gacagcatga gggtacatgc aacgtttcta tggctgctct
     1741 tgataaattt gtggccgaac gtattttcaa caagattaga cacgctgaag gggatgagga
     1801 aactttagct cttctttggg aggctgcccg gcgttttggt aagctaacag aggcaccaga
     1861 aaaaagtggc gaaagagcta atttggtagc cgaaagagca gacgccttga atgcattgga
     1921 agaactttac gaagataggg ctgccggagc atatgatgga ccagttggtc ggaagcattt
     1981 caggaagcaa caagctgcac taactttgag acagcaaggc gcagaggagc gactcgcaga
     2041 gcttgaggcc gccgaggccc caaagcttcc actcgatcaa tggtttcctg aggatgccga
     2101 cgctgatcct acaggtccaa agagctggtg gggaagagct agcgtagacg ataagagagt
     2161 gtttgtgggt ctatttgttg acaagattgt agttactaag tccactacgg gtcgaggaca
     2221 gggcactccc atcgaaaaga gagccagtat tacctgggca aagcctccaa ccgatgatga
     2281 tgaagacgat gcacaagatg gtaccgagga tgtggcagca ccaaaaaaaa agaggaaggt
     2341 gtaagcttgg aatggatctt cgatcccgat cgttcaaaca tttggcaata aagtttctta
     2401 agattgaatc ctgttgccgg tcttgcgacg attatcatat aatttctgtt gaattacgtt
     2461 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     2521 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     2581 gataaattat cgcgcdcggt gtcatctatg ttactagatc gggaattgcc aagctaattc
     2641 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2701 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2761 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2821 tcaataatgg gaccgactcg cgctgtcatg agacgaattc tgacaggata tattggcggg
     2881 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     2941 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     3001 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     3061 cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     3121 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     3181 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     3241 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     3301 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     3361 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     3421 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     3481 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     3541 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     3601 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     3661 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     3721 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     3781 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     3841 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     3901 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     3961 gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg
     4021 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4081 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4141 gtatatgatc cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     4201 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg gaacccatct agcccgcgcc
     4261 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     4321 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     4381 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     4441 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     4501 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     4561 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     4621 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     4681 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     4741 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     4801 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     4861 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     4921 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     4981 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatag atcgcgcagc
     5041 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5101 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     5161 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     5221 acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg
     5281 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     5341 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     5401 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     5461 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     5521 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     5581 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     5641 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     5701 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     5761 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     5821 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     5881 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     5941 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     6001 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     6061 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     6121 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     6181 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     6241 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     6301 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     6361 aggtcgaaaa ggactctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     6421 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     6481 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     6541 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     6601 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     6661 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     6721 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     6781 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggtgacgg
     6841 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     6901 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     6961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7021 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggtg
     7921 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     7981 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     8041 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     8101 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     8161 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     8221 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     8281 gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     8341 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     8401 attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat
     8461 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     8521 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     8581 acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga
     8641 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     8701 gccttgagca agacgtttcc cgttgaatat ggctcataac agaacttatt atttccttcc
     8761 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     8821 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     8881 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
     8941 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     9001 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     9061 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     9121 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
     9181 attgtggtgt aaacataacg aattcgtctc a
//
