LOCUS       sequence-31100-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Nishino J, Kim I, Chada K, Morrison SJ
  TITLE     Hmga2 promotes neural stem cell self-renewal in young but not old 
            mice by reducing p16Ink4a and p19Arf Expression.
  JOURNAL   Cell. 2008 Oct 17. 135(2):227-39.
  PUBMED    18957199
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Oct 17. 135(2):227-39."
COMMENT     Sequence Label: HMGA2 shRNA
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           75..196
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           224..238
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           266..392
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        546..648
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(715..734)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcggcatgga cgagctgtac aagtagcggc cgcaagcctt gttaagtgct cgcttcggca
       61 gcacatatac tatgttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt
      121 ggaaacactt gctgggatta cttcttcagg ttaacccaac agaaggctcg agaaggtata
      181 ttgctgttga cagtgagcga tttgtggatc tgataagcaa gtagtgaagc cacagatgta
      241 cttgcttatc agatccacaa actgcctact gcctcggaat tcaaggggct actttaggag
      301 caattatctt gtttactaaa actgaatacc ttgctatctc tttgatacat ttttacaaag
      361 ctgaattaaa atggtataaa ttaaatcact tttttcaatt ggaagactaa tgcgtttaaa
      421 cacgcggcga cgcgttcgac cgaataaata cctgtgacgg aagatcactt cgcagaataa
      481 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga
      541 gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
      601 ggcgtatttt ttgagttgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      661 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      721 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      781 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      841 cgcctgatga atgctcatcc ggaattacgt atggcaatga aagacggtga gctggtgata
      901 tgggatagtg ttcacccttg ttacaccgtt ttccatganc aaactgaaac gttttcatcg
      961 ctct
//
