LOCUS       sequence-31106-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Tominaga T, Sahai E, Chardin P, McCormick F, Courtneidge SA, Alberts
            AS
  TITLE     Diaphanous-related formins bridge Rho GTPase and Src tyrosine kinase
            signaling.
  JOURNAL   Mol Cell. 2000 Jan . 5(1):13-25.
  PUBMED    10678165
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2000 Jan . 5(1):13-25."
COMMENT     Sequence Label: pEFmEGFP-mDia2
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..57)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(67..84)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          165..368
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(188..208)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ttctccttta ctagatccgg ccatggccag gtcctcctcg gatatcagct tctgctccgg
       61 catggcgtga tgatggtggt gatgcggcat ggtgtctgtt tgaggttgct agtgaacaca
      121 gttgtgtcag aagcaaatgt aagcaagcta attcctcacg acacctgaaa tggaagaaaa
      181 aaactttgaa ccactgtctg aggcttgaga atgaaccaag atccaaactc aaaaagggca
      241 aattccaagg agaattacat caagtgccaa gctggcctaa cttcagtctc cacccactca
      301 gtgtggggaa actccatcgc ataaaacccc tccccccaac ctaaagacga cgtactccaa
      361 aagctcgaga actaatcgag gtgcctggac ggcgcccggt actccgtgga gtcacatgaa
      421 gcgacggctg aggacggaaa ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct
      481 cccgagcgcc gcgtcctcca ttttgagctc cctgcagcag ggccgggaag cggccatctt
      541 tccgctcacg caactggtgc cgaccgggcc agccttgccg cccagggcgg ggcgatacac
      601 ggcggcgcga ggccaggcac cagagcaggc cggccagctt gagactaccc ccgtccgatt
      661 ctcggtggcc gcgctcgcag gccccgcctc gccgaacatg tgcgctggga cgcacgggcc
      721 ccgtcgccgc ccgcggcccc aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt
      781 acaagactat cttgccagaa aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct
      841 agagacttat cgaaagcagc g
//
