LOCUS       sequence-311068-        8691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of PULSE-controlled GBP-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pNBA002
FEATURES             Location/Qualifiers
     source          1..8691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     538..562
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1131..1835
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    2126..2150
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3451..4080
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4509..5582
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5648..5842
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6078..6100)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6186..6326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6241..6260
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6341..6358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6512..7100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6592..6611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7523..8314
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga cggattacgg aagacaatac
       61 ggcggcttcg aagtttaaac gattgaatat aaccgacgtg actgttacat ttagggattg
      121 aatataaccg acgtgactgt tacatttagg gattgaatat aaccgacgtg actgttacat
      181 ttagggattg aatataaccg acgtgactgt tacatttagg gataaacgat tgaatataac
      241 cgacgtgact gttacattta gggattgaat ataaccgacg tgactgttac atttagggat
      301 tgaatataac cgacgtgact gttacattta gggattgaat ataaccgacg tgactgttac
      361 atttagggat gctagcctcg agtaggtagc ctttagtcca tgcgttatag gtagccttta
      421 gtccatgcgt tataggtagc ctttagtcca tgcgttatag gtagccttta gtccatgcgt
      481 tataggtagc ctttagtcca tgaagcttag acactagagc tagccctata taagcagagc
      541 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      601 aagacaccgg gaccgatcca gccttctctg gaagcacctg acctaagcta gcctagaagg
      661 gaatggcgga tgtgaacctg gtggaaagcg gcggcgcgct ggtgaacccg ggcggcagcc
      721 tgcgcctgag ctgcgcggcg agcggctttc cggtgcagcg ctatagcatg cgctggtggc
      781 gcaacgcgcc gggcaaagaa cgcgaatggg tggcgggcat gagcagcgcg ggcgatcgca
      841 gcagctatga agatagcgtg aaaggccgct ttaccattag ccgcgatgat gcgcgcaaca
      901 ccgtgtatct gaacatgaac agcctgaaac cggaagatac cgcggtgtat tattgcaacg
      961 tgaacgtggg ctttgaatat tggggcaacg gcaccaacgt gaccgtgagc agccgcaaca
     1021 gcattagcag cctgagcatt ccgagcaccg tgccgcgcgc gcgcgatccg ccggtggata
     1081 agggaggtgg aggaggttct ggaggcggtg gaagtggtgg cggaggtagc gtgagcaagg
     1141 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1201 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1261 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     1321 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     1381 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1441 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     1501 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaaa
     1561 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     1621 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     1681 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     1741 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     1801 gccactccac cggcggcatg gacgagctgt acaagaatcc ggccatgcta gagtccgcaa
     1861 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     1921 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     1981 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     2041 tcctaaaacc aaaatccagt gaccttgagt gacctaagct agcctagtgt cgggcttgtc
     2101 ttctctggtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     2161 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     2221 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     2281 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccttct tctgaaaacg
     2341 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     2401 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     2461 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     2521 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     2581 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     2641 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     2701 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     2761 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     2821 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     2881 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     2941 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     3001 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     3061 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     3121 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     3181 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     3241 gcccgcgcac atctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     3301 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     3361 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     3421 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     3481 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     3541 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     3601 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     3661 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     3721 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     3781 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     3841 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     3901 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     3961 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     4021 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     4081 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     4141 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     4201 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     4261 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     4321 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     4381 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     4441 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     4501 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     4561 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     4621 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     4681 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     4741 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     4801 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     4861 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     4921 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     4981 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     5041 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     5101 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     5161 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     5221 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     5281 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     5341 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     5401 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     5461 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     5521 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     5581 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
     5641 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     5701 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
     5761 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     5821 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     5881 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     5941 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     6001 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     6061 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacagtcac agcttgtctg
     6121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     6181 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     6241 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6301 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     6361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcatc
     7141 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     7201 gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg
     7261 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     7321 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     7381 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     7441 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     7501 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
     7561 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     7621 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     7681 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     7741 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     7801 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     7861 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     7921 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     7981 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     8041 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     8101 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     8161 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     8221 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     8281 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     8341 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     8401 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     8461 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     8521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct gccggtaaca tgagcaaagt
     8581 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     8641 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//
