LOCUS       sequence-311072-        5178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PULSE-controlled FLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pROF021
FEATURES             Location/Qualifiers
     source          1..5178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     533..557
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             702..2354
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(768..786)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3098..3219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3135..3154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3189..3208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3253..3270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3424..4012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3504..3523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4183..4974,4975..5043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4806..4825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5044..5148)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcgacgat cgacctgcag gtaggcgtgt acggtgggag gcctaggctt ttgcaaaaag
       61 cttcgaagtt taaacgattg aatataaccg acgtgactgt tacatttagg gattgaatat
      121 aaccgacgtg actgttacat ttagggattg aatataaccg acgtgactgt tacatttagg
      181 gattgaatat aaccgacgtg actgttacat ttagggataa acgattgaat ataaccgacg
      241 tgactgttac atttagggat tgaatataac cgacgtgact gttacattta gggattgaat
      301 ataaccgacg tgactgttac atttagggat tgaatataac cgacgtgact gttacattta
      361 gggatgctag cctcgagtag gtagccttta gtccatgcgt tataggtagc ctttagtcca
      421 tgcgttatag gtagccttta gtccatgcgt tataggtagc ctttagtcca tgcgttatag
      481 gtagccttta gtccatgaag cttagacact agagctagcc ctatataagc agagctcgtt
      541 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      601 accgggaccg atccagcctc cgcggccccg gtaccgaatt cgagctcgcc cggggatcct
      661 ctagagtcag cttccgctca attggctgcg ctggcgctag catggaagac gccaaaaaca
      721 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
      781 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
      841 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
      901 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
      961 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     1021 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     1081 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     1141 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     1201 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     1261 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     1321 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     1381 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     1441 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     1501 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     1561 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     1621 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     1681 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     1741 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     1801 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     1861 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     1921 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     1981 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     2041 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     2101 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     2161 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     2221 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     2281 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     2341 agatcgccgt gtaagcggcc gcggccggcc cgggcgcgcc tgatcaatcg atgtttaaac
     2401 ttaaaacagc tctggggttg tacccacccc agaggcccac gtggcggcta gtactccggt
     2461 attgcggtac ccttgtacgc ctgttttata ctcccttccc gtaacttaga cgcgcaaaac
     2521 caagttcaat agaagggggt acaaaccagt accaccacga acaagcactt ctgtttcccc
     2581 ggtgatgtcg tatagactgc ttgcgtggtt gaaagcgacg gatccgttat ccgcttatgt
     2641 acttcgagaa gcccagtacc acctcggaat cttcgatgcg ttgcgctcag cactcaaccc
     2701 cagagtgtag cttaggctga tgagtctgga catccctcac cggtgacggt ggtccaggct
     2761 gcgttggcgg cctacctatg gctaacgcca tgggacgcta gttgtgaaca aggtgtgaag
     2821 agcctattga gctacataag aatcctccgg cccctgaatg cggctaatcc caacctcgga
     2881 gcaggtggtc acaaaccagt gattggcctg tcgtaacgcg caagtccgtg gcggaaccga
     2941 ctactttggg tgtccgtgtt tccttttatt ttattgtggc tgcttatggt gacaatcaca
     3001 gattgttatc ataaagcgaa ttggattctc gagactagtg cgatcgctta attaatttaa
     3061 atacgcgttt aaacagatcc agatcttatt aaagcagaac ttgtttattg cagcttataa
     3121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgactctaga
     3241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5161 aaaagtgcca cctgacgt
//
