LOCUS       sequence-311104-        8810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PFAS-mMaple3 fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Chan CY, Pedley AM, Kim D, Xia C, Zhuang X, Benkovic SJ
  TITLE     Microtubule-directed transport of purine metabolons drives their 
            cytosolic transit to mitochondria.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Dec 18;115(51):13009-13014. doi: 
            10.1073/pnas.1814042115. Epub 2018 Dec 3.
  PUBMED    30509995
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814042115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-12-18- 18"; volume: "115"; issue: "51"; pages: 
            "13009-13014"
COMMENT     Sequence Label: pFGAMS-mMaple3
FEATURES             Location/Qualifiers
     source          1..8810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4696..4705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5538..5659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5575..5594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5629..5648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5666..6121)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5803..5824)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6015..6034
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6148..6252
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6254..6611
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6254..6445
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6277..6297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6462..6597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6646..7440
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6646..7440
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6700..6719)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7310..7329
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7628..7647)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7672..7719
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8048..8636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8537..8556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgc
      541 gcgagtgcat cttccacgaa cctaattcat ctctccagca aaggacacat ctctccagca
      601 aaggacacct ctctccagca aaggacacct gcagagatgt ccccagtcct tcacttctat
      661 gttcgtccct ctggccatga gggggcagcc cctggacaca ctcggaggaa actgcaaggg
      721 aaactgccag agctgcaggg cgtcgagact gaactgtgct acaacgtgaa ctggacagct
      781 gaggccctcc ccagtgctga ggagacaaag aagctgatgt ggctgtttgg ttgcccctta
      841 ctgctggatg atgttgctcg ggagtcctgg ctccttcctg gctccaatga cctgctgctg
      901 gaggtcgggc ccaggctgaa cttctccacc ccaacatcca ccaacatcgt gtcagtgtgc
      961 cgcgccactg ggctggggcc tgtggatcgt gtggagacca cccggcgcta ccggctctcg
     1021 tttgcccacc ccccgtcagc tgaggtggaa gccattgctc tggctaccct gcacgaccgg
     1081 atgacagagc agcacttccc ccatcccatc cagagtttct cccctgagag catgccggaa
     1141 cccctcaatg gccctatcaa tatactgggt gagggccggc ttgcgctgga gaaggccaac
     1201 caggagcttg gtctggcttt agactcttgg gacctagact tctacaccaa gcgcttccag
     1261 gagctacagc ggaacccgag cactgtggag gcctttgact tggcgcagtc caatagcgag
     1321 cacagccgac actggttctt caagggccag ctccacgtgg atgggcagaa gctggtgcac
     1381 tcactgtttg agtccatcat gagcacccag gaatcctcga accccaacaa cgtcctcaaa
     1441 ttctgtgata acagcagtgc aatccaggga aaggaagtcc gattcctacg gcctgaggac
     1501 cccacacggc caagccgctt ccagcaacag caagggctga gacatgttgt cttcacagca
     1561 gagactcaca actttcccac aggagtatgc ccctttagtg gtgcaaccac tggcacaggg
     1621 ggccggattc gagatgtcca gtgcacaggc cgcggggccc acgtggtggc tggcactgcc
     1681 ggctattgct ttggaaatct gcatattcca ggttacaatc tgccctggga ggatccaagc
     1741 ttccagtatc ctgggaattt tgcccggccc ctggaggttg ccattgaagc cagtaatgga
     1801 gcttctgact atggcaacaa gtttggggaa ccagtgctgg ctggcttcgc ccgctccttg
     1861 ggcctccagc tcccagacgg ccagcggcgt gagtggatca agcccatcat gtttagtggg
     1921 ggcattgggt ccatggaagc tgaccacata agcaaggagg ccccagagcc aggcatggaa
     1981 gttgtaaagg ttggaggtcc cgtctacagg attggagttg gaggtggagc tgcttcatct
     2041 gtgcaggtgc agggagataa caccagtgac ctggactttg gggctgtgca gcgaggagac
     2101 ccggagatgg aacagaagat gaaccgtgtg atcagggctt gtgtggaggc ccccaaggga
     2161 aaccccatct gcagccttca tgatcagggc gctggtggca atggcaatgt cctaaaagag
     2221 ctgagtgacc cagctggagc catcatttac accagccgct tccagcttgg ggacccaacc
     2281 ctgaatgccc tggaaatctg gggggctgag taccaggaat caaatgctct tctgctgagg
     2341 tcccccaacc gggacttcct gactcatgtc agtgcccgtg aacgttgccc ggcttgcttc
     2401 gtgggcacca tcactggaga ccggagaata gtgctggtgg acgatcggga gtgtcctgtc
     2461 agaagaaatg gccaggggga tgcccccccg acacccctgc caacccctgt ggacctggag
     2521 ctcgaatggg tgctgggcaa gatgcctcgg aaggagttct tcctgcagag gaagcccccc
     2581 atgctgcagc ctctggcctt gcccccaggg ctgagcgtgc accaggctct ggagagggtt
     2641 ctgaggctgc ccgccgtggc cagcaagcgc tacctcacca ataaggtgga ccgctccgtg
     2701 ggaggcctgg tggcccagca gcagtgcgtg gggcccctgc aaactcctct ggcagatgta
     2761 gcggttgtgg cactgagcca tgaggagctc ataggggctg ccacagcctt gggagaacag
     2821 ccagtcaaga gcctgctgga cccaaaagtc gccgcccggc tggccgtggc cgaagccctc
     2881 accaacctgg tgtttgctct ggtcactgac ctccgggatg tgaagtgtag cgggaactgg
     2941 atgtgggcag ccaagctccc aggggagggc gcagctttgg cggatgcctg tgaggctatg
     3001 gtggcagtga tggcagccct gggtgtggca gtggatggtg gcaaggactc cctcagcatg
     3061 gctgctcggg ttggcactga gaccgtgcgg gctcctgggt cactggtcat ctcagcctat
     3121 gccgtctgcc cagacatcac agccactgtg accccagacc tcaagcatcc tgaagggaga
     3181 ggccatctgc tctatgtggc tctgagccct gggcagcacc ggctcggggg cacagctctg
     3241 gcccagtgct tctcccagct tggggaacac cctccagacc tggaccttcc tgagaacttg
     3301 gtgcgggcct tcagcatcac tcaggggctg ctgaaagacc gcctcctctg ctcaggccac
     3361 gatgtcagtg acggaggcct cgtcacatgc ctgctggaga tggcctttgc tggaaattgc
     3421 gggctacagg tggatgtgcc tgtccccagg gttgatgtcc tgtctgtgct gttcgctgag
     3481 gagccaggcc tcgtgctgga ggtgcaggag ccagacctgg cccaggtgct gaagcgttac
     3541 cgggatgctg gcctccattg cctggagctg ggccacacag gcgaggccgg gccccacgcc
     3601 atggtccggg tgtcagtgaa cggggctgtg gttctggagg agcctgttgg ggagctgcga
     3661 gccctctggg aggagacgag tttccagctg gaccggctac aggcagagcc tcgctgtgtg
     3721 gcagaggagg aacggggcct gagggagcgg atggggccca gctattgcct gccccccacc
     3781 tttcccaaag cctccgtgcc ccgtgagcct ggtggtccca gcccccgagt cgccatcttg
     3841 cgagaggagg gcagtaatgg agaccgggag atggccgatg ccttccactt agctgggttt
     3901 gaggtatggg acgtgaccat gcaggacctc tgctctgggg caattgggct ggacactttc
     3961 cgtggcgtgg ccttcgtggg cggcttcagc tatgcagatg tcctgggctc tgccaaaggg
     4021 tgggcagctg ctgtgacctt tcatcccagg gctggggctg agctgaggcg cttccggaag
     4081 cggccagaca ccttcagcct gggcgtgtgt aatggctgtc aactgctggc tctgctcggc
     4141 tgggtgggag gcgaccccaa tgaggatgct gcagagatgg gccctgactc ccagccagcc
     4201 cggccaggcc ttctgctacg ccacaacctg tctgggcgct acgagtctcg ctgggccagc
     4261 gtgcgtgtgg ggcctgggcc agccctgatg ctgcgaggga tggagggcgc cgtgctgccc
     4321 gtgtggagtg cgcacgggga aggttacgta gcattttctt ctccggaact ccaagctcag
     4381 attgaggcca ggggcttggc tccactgcac tgggctgatg atgacgggaa ccccacagag
     4441 cagtaccctc tgaatcccaa tgggtcccca gggggcgtgg ctggcatctg ctcctgtgat
     4501 ggccgccacc tggctgtcat gcctcaccct gagcgggccg ttaggccttg gcagtgggca
     4561 tggcgacccc ctccatttga tactctgacc acctccccct ggctccagct ctttatcaat
     4621 gcccgaaact ggaccctgga agggagctgc ggaattctgc agtcgacggt accgcgggcc
     4681 cgggatccac cggtcgccac catggtgagc aaaggcgagg agacaatcat gtccgtgatc
     4741 aagcccgaca tgaagatcaa actgaggatg gagggcaacg tgaacggcca cgccttcgtg
     4801 atcgagggcg aaggaagcgg caagcccttc gagggcatcc agaccatcga tctggaggtc
     4861 aaggagggcg ctcccctccc tttcgcctat gacatcctga ccaccgcctt ccactacggc
     4921 aatagggtgt tcaccaagta tcccaggaag atccccgact acttcaagca gagcttccct
     4981 gagggctaca gctgggagag gagcatgaca tacgaggacg gcggcatctg caacgccacc
     5041 aacgacatca caatggagga ggacagcttc atcaacaaga tccacttcaa aggcacaaac
     5101 ttccccccca atggccccgt gatgcagaag aggaccgtgg gctgggaggt gagcaccgag
     5161 aagatgtacg tgagggacgg cgtcctgaag ggcgacgtga agatgaagct cctgctcaag
     5221 ggcggcagcc actacaggtg cgactttagg accacctata aggtgaagca gaaggctgtg
     5281 aagctgccca aggcccactt cgtcgaccat aggatcgaga tcctgtccca cgacaaggac
     5341 tacaacaagg tcaagctgta cgagcacgcc gtcgctagga acagcaccga cagcatggac
     5401 gaactctata agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     5461 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5521 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5641 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     5701 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5761 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5821 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5881 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5941 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     6001 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     6061 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     6121 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6181 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6241 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     6301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6361 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6421 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6481 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6541 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6601 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6661 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6721 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6781 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6841 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6901 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6961 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     7021 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7081 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7141 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     7201 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7261 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7321 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7381 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7441 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7501 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7561 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     7621 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7681 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7741 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7801 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7861 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     8761 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
