LOCUS       sequence-311105-       17644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of PULSE system (under the control of 
            AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses
            Cerulean-NLS and a plant selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 17644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pROF346
FEATURES             Location/Qualifiers
     source          1..17644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     548..572
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(645..647,648..650,651..1361)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1860..2034
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2728..2748
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3403..3655
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             6464..6697
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             6698..6718
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6734..6986
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             join(8534..8536,8537..8539,8540..9250)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             9260..9280
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9304..9556
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             11156..11176
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11192..11444
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        11547..11726
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             11748..12542
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11802..11821)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12412..12431
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      12555..12807
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    12902..12926
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13190..13819
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14248..15321
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15387..15581
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        15864..15968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             15969..16763
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      16934..17522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17423..17442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactccctcg gtcacatgtg
       61 catcctcgct cggagcttcg aagtttaaac gattgaatat aaccgacgtg actgttacat
      121 ttagggattg aatataaccg acgtgactgt tacatttagg gattgaatat aaccgacgtg
      181 actgttacat ttagggattg aatataaccg acgtgactgt tacatttagg gataaacgat
      241 tgaatataac cgacgtgact gttacattta gggattgaat ataaccgacg tgactgttac
      301 atttagggat tgaatataac cgacgtgact gttacattta gggattgaat ataaccgacg
      361 tgactgttac atttagggat gctagcctcg agtaggtagc ctttagtcca tgcgttatag
      421 gtagccttta gtccatgcgt tataggtagc ctttagtcca tgcgttatag gtagccttta
      481 gtccatgcgt tataggtagc ctttagtcca tgaagcttag acactagagc tagccctata
      541 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      601 acctccatag aagacaccgg gaccgatcca gcctaatgac tagtatggtg agcaagggcg
      661 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      721 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      781 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
      841 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      901 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      961 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1021 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1081 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     1141 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     1201 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     1261 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1321 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gttaatcgca atggcgaagg
     1381 cagataagaa acctgcggag aagaaaccgg cagagaaaac tccggcagtc gaaccagcag
     1441 cagcggcaga gaagaaacca aaagccggaa agaaactccc caaggaacca gccgtcgccg
     1501 gagacaagaa gaagaagaga tcaaagaaga acgttgagac atacaagatc tacatcttca
     1561 aggtgttgaa gcaagttcat ccagacatcg gaatctcaag caaagccatg ggaatcatga
     1621 acagtttcat caacgatatc tttgagaagc ttgctggtga gtcttcgaag cttgcgaggt
     1681 acaacaagaa gccgacgatt acttccaggg agattcagac tgcggttaga cttgtgttgc
     1741 ccggtgagtt ggcgaaacat gctgtgtctg aagggactaa ggcggttacg aagtttacga
     1801 gttctttaat taactagcct gcagggcttc tctagctaga gtcgatcgac aagctcgagt
     1861 ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt
     1921 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     1981 tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcgctctc
     2041 gaggaattcc acaggaggac gagtcagtaa taaacggcgt caaagtggtt gcagccggca
     2101 cacacgagtc gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg
     2161 caattagcca aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat
     2221 tagctattgc ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt
     2281 cttcttctat aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc
     2341 tcaaaatctt aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt
     2401 tccttattct ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc
     2461 tttggtttag attctgttaa tcttagatcg tagacgattt tctgggtttg atcgttagat
     2521 atcatcttaa ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga
     2581 gttttgtcga ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct
     2641 agtttgtgcg atcgaatttg tcgattaatc tgagtttttc tgattaacag aatgatgctt
     2701 gatcttgaac ttagacttgg ttttgctcca aagaagaaga ggaaggtggg agctggagct
     2761 ggggcagacg acacacgcgt tgaggtgcaa ccgccggcgc agtgggtcct cgacctgatc
     2821 gaggccagcc cgatcgcatc ggtcgtgtcc gatccgcgtc tcgccgacaa tccgctgatc
     2881 gccatcaacc aggccttcac cgacctgacc ggctattccg aagaagaatg cgtcggccgc
     2941 aattgccgat tcctggcagg ttccggcacc gagccgtggc tgaccgacaa gatccgccaa
     3001 ggcgtgcgcg agcacaagcc ggtgctggtc gagatcctga actacaagaa ggacggcacg
     3061 ccgttccgca atgccgtgct cgttgcaccg atctacgatg acgacgacga gcttctctat
     3121 ttcctcggca gccaggtcga agtcgacgac gaccagccca acatgggcat ggcgcgccgc
     3181 gaacgcgccg cggaaatgct caagacgctg tcgccgcgcc agctcgaggt tacgacgctg
     3241 gtggcatcgg gcttgcgcaa caaggaagtg gcggcccggc tcggcctgtc ggagaaaacc
     3301 gtcaagatgc accgcgggct ggtgatggaa aagctcaacc tgaagaccag tgccgatctg
     3361 gtgcgcattg ccgtcgaagc cggaatctaa gcttgtcaag cagatcgttc aaacatttgg
     3421 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     3481 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     3541 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     3601 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgacgc
     3661 tgcatgcgaa ttctctggga ggacgagtca gtaataaacg gcgtcaaagt ggttgcagcc
     3721 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     3781 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     3841 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     3901 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     3961 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     4021 gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     4081 gttctttggt ttagattctg ttaatcttag atcgtagacg attttctggg tttgatcgtt
     4141 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     4201 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     4261 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagaatgac
     4321 aacaatggtt tccggagtcg ggggtagtgg cggtggccgt ggcggtggcc gtggcggaga
     4381 agaagaaccg tcgtcaagtc acactcctaa taaccgaaga ggaggagaac aagctcaatc
     4441 gtcgggaacg aaatctctca gaccaagaag caacactgaa tcaatgagca aagcaattca
     4501 acagtacacc gtcgacgcaa gactccacgc cgttttcgaa caatccggcg aatcagggaa
     4561 atcattcgac tactcacaat cactcaaaac gacgacgtac ggttcctctg tacctgagca
     4621 acagatcaca gcttatctct ctcgaatcca gcgaggtggt tacattcagc ctttcggatg
     4681 tatgatcgcc gtcgatgaat ccagtttccg gatcatcggt tacagtgaaa acgccagaga
     4741 aatgttaggg attatgcctc aatctgttcc tactcttgag aaacctgaga ttctagctat
     4801 gggaactgat gtgagatctt tgttcacttc ttcgagctcg attctactcg agcgtgcttt
     4861 cgttgctcga gagattacct tgttaaatcc ggtttggatc cattccaaga atactggtaa
     4921 accgttttac gccattcttc ataggattga tgttggtgtt gttattgatt tagagccagc
     4981 tagaactgaa gatcctgcgc tttctattgc tggtgctgtt caatcgcaga aactcgcggt
     5041 tcgtgcgatt tctcagttac aggctcttcc tggtggagat attaagcttt tgtgtgacac
     5101 tgtcgtggaa agtgtgaggg acttgactgg ttatgatcgt gttatggttt ataagtttca
     5161 tgaagatgag catggagaag ttgtagctga gagtaaacga gatgatttag agccttatat
     5221 tggactgcat tatcctgcta ctgatattcc tcaagcgtca aggttcttgt ttaagcagaa
     5281 ccgtgtccga atgatagtag attgcaatgc cacacctgtt cttgtggtcc aggacgatag
     5341 gctaactcag tctatgtgct tggttggttc tactcttagg gctcctcatg gttgtcactc
     5401 tcagtatatg gctaacatgg gatctattgc gtctttagca atggcggtta taatcaatgg
     5461 aaatgaagat gatgggagca atgtagctag tggaagaagc tcgatgaggc tttggggttt
     5521 ggttgtttgc catcacactt cttctcgctg cataccgttt ccgctaaggt atgcttgtga
     5581 gtttttgatg caggctttcg gtttacagtt aaacatggaa ttgcagttag ctttgcaaat
     5641 gtcagagaaa cgcgttttga gaacgcagac actgttatgt gatatgcttc tgcgtgactc
     5701 gcctgctgga attgttacac agagtcccag tatcatggac ttagtgaaat gtgacggtgc
     5761 agcatttctt taccacggga agtattaccc gttgggtgtt gctcctagtg aagttcagat
     5821 aaaagatgtt gtggagtggt tgcttgcgaa tcatgcggat tcaaccggat taagcactga
     5881 tagtttaggc gatgcggggt atcccggtgc agctgcgtta ggggatgctg tgtgcggtat
     5941 ggcagttgca tatatcacaa aaagagactt tcttttttgg tttcgatctc acactgcgaa
     6001 agaaatcaaa tggggaggcg ctaagcatca tccggaggat aaagatgatg ggcaacgaat
     6061 gcatcctcgt tcgtcctttc aggcttttct tgaagttgtt aagagccgga gtcagccatg
     6121 ggaaactgcg gaaatggatg cgattcactc gctccagctt attctgagag actcttttaa
     6181 agaatctgag gcggctatga actctaaagt tgtggatggt gtggttcagc catgtaggga
     6241 tatggcgggg gaacagggga ttgatgagtt aggtgaattc gatagtgctg gtagtgctgg
     6301 tagtgctggt tccgcgtaca gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga
     6361 gggcctgctc gatctcccgg acgacgacgc ccccgaagag gcggggctgg cggctccgcg
     6421 cctgtccttt ctccccgcgg gacacacgcg cagactgtcg acggcccccc cgaccgatgt
     6481 cagcctgggg gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc
     6541 gctagacgat ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac
     6601 cccccacgac tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat
     6661 gtttaccgat gcccttggaa ttgacgagta cggtgggcca aagaagaaga ggaaggtgta
     6721 ggcttgtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     6781 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     6841 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     6901 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     6961 cgcggtgtca tctatgttac tagatcgacg ctccatggga attcgtaagg aggacgtcgt
     7021 tgtggttggt gctttcctta cattctgagc ctctttcctt ctaatccact catctgcatc
     7081 ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa gcaccagctc
     7141 gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca cattcttcag
     7201 atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa ctgatgatct
     7261 aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt tgtgtatcat
     7321 tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa cacgagtgtt
     7381 aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa caagaataaa
     7441 tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt gatacaaaag
     7501 tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta aaaaaattaa
     7561 aagaaatgga taatttcaca atatgttata cgataaagaa gttacttttc caagaaattc
     7621 actgatttta taagcccact tgcattagat aaatggcaaa aaaaaacaaa aaggaaaaga
     7681 aataaagcac gaagaattct agaaaatacg aaatacgctt caatgcagtg ggacccacgg
     7741 ttcaattatt gccaattttc agctccaccg tatatttaaa aaataaaacg ataatgctaa
     7801 aaaaatataa atcgtaacga tcgttaaatc tcaacggctg gatcttatga cgaccgttaa
     7861 ggaaattgtg gttgtcggac gaagtccagt aataaacggc gtcaaagtgg ttgcagccgg
     7921 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     7981 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     8041 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     8101 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     8161 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     8221 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     8281 tctttggttt agattctgtt aatcttagat cgtagacgat tttctgggtt tgatcgttag
     8341 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     8401 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     8461 ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agatgcagat
     8521 cttaatgtcg cgaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     8581 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     8641 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     8701 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc
     8761 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     8821 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     8881 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     8941 catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga
     9001 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
     9061 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     9121 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     9181 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     9241 gctgtacaag ttaattaacc ccaagaaaaa gcggaaggtg tgagcgatcg cgcttgtcaa
     9301 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     9361 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     9421 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     9481 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     9541 tctatgttac tagatcgacg ctgagctcga attctagtgg aggacgagtc agtaataaac
     9601 ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat
     9661 acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg
     9721 ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac
     9781 ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct
     9841 tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta
     9901 ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg
     9961 ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta gatcgtagac
    10021 gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat
    10081 catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt
    10141 ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt
    10201 ttttctgatt aacagaatga caacaatgcc ccgccccaag ctcaagtccg atgacgaggt
    10261 actcgaggcc gccaccgtag tgctgaagcg ttgcggtccc atagagttca cgctcagcgg
    10321 agtagcaaag gaggtggggc tctcccgcgc agcgttaatc cagcgcttca ccaaccgcga
    10381 tacgctgctg gtgaggatga tggagcgcgg cgtcgagcag gtgcggcatt acctgaatgc
    10441 gataccgata ggcgcagggc cgcaagggct ctgggaattt ttgcaggtgc tcgttcggag
    10501 catgaacact cgcaacgact tctcggtgaa ctatctcatc tcctggtacg agctccaggt
    10561 gccggagcta cgcacgcttg cgatccagcg gaaccgcgcg gtggtggagg ggatccgcaa
    10621 gcgactgccc ccaggtgctc ctgcggcagc tgagttgctc ctgcactcgg tcatcgctgg
    10681 cgcgacgatg cagtgggccg tcgatccgga tggtgagcta gctgatcatg tgctggctca
    10741 gatcgctgcc atcctgtgtt taatgtttcc cgaacacgac gatttccaac tcctccaggc
    10801 acatgcgtcc gcgtacagct taattaacag tgctggtagt gctggtagtg ctggtatgat
    10861 gttcttacca accgattatt gttgcaggtt aagcgatcaa gagtatatgg agcttgtgtt
    10921 tgagaatggc cagattcttg caaagggcca aagatccaac gtttctctgc ataatcaacg
    10981 taccaaatcg atcatggatt tgtatgaggc agagtataac gaggatttca tgaagagtat
    11041 catccatggt ggtggtggtg ccatcacaaa tctcggggac acgcaggttg ttccacaaag
    11101 tcatgttgct gctgcccatg aaacaaacat gttggaaagc aataaacatg ttgacccaaa
    11161 gaagaagagg aaggtgtagg cttgtcaagc agatcgttca aacatttggc aataaagttt
    11221 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
    11281 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
    11341 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
    11401 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct aagcttgaat
    11461 tcttgcggag agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac
    11521 aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcgg
    11581 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
    11641 tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc
    11701 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaaatg attgaacaag
    11761 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    11821 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    11881 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccaa gacgaggcag
    11941 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    12001 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    12061 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    12121 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgaggac
    12181 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    12241 tcgcgccagc cgaactgttc gccaggctca aggcgcggat gcccgacggc gaggatctcg
    12301 tcgtgaccca gggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    12361 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    12421 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    12481 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    12541 gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    12601 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    12661 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    12721 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    12781 gcgcggtgtc atctatgtta ctagatcgac gctggatccg aattcggcag ggcccactgc
    12841 atccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
    12901 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
    12961 gcacaaaatc accactcgat acaggcagcc catcagccgt gcggctgcat gaaatcctgg
    13021 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
    13081 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
    13141 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
    13201 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
    13261 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
    13321 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
    13381 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
    13441 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
    13501 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
    13561 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
    13621 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
    13681 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
    13741 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
    13801 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
    13861 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
    13921 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
    13981 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
    14041 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
    14101 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
    14161 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
    14221 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
    14281 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
    14341 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
    14401 atccgcaaag aatcccggca accgccggca gccggtgcgt cgtcgattag gaagccgccc
    14461 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
    14521 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
    14581 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
    14641 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
    14701 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
    14761 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
    14821 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
    14881 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
    14941 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
    15001 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
    15061 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
    15121 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
    15181 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
    15241 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
    15301 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
    15361 gaaaaaggtc gaaaaagctt ctttcctgtg gatagcacgt acattgggaa cccaaagccg
    15421 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
    15481 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
    15541 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
    15601 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
    15661 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
    15721 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
    15781 accgagctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa
    15841 atggcagcgc tggccaattc gtgcgcggaa cccctatttg tttatttttc taaatacatt
    15901 caaatatgta tccgctcatg agacaataac cttgataaat gcttcaataa tattgaaaaa
    15961 ggaagagtat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
    16021 gctgcgcaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
    16081 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
    16141 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
    16201 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
    16261 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
    16321 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
    16381 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
    16441 actgggaaga ggacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
    16501 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
    16561 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
    16621 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
    16681 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
    16741 ttttactgga tgaattgttt tagctgtcag accaagttta ctcatatata ctttagattg
    16801 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    16861 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    16921 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    16981 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    17041 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    17101 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    17161 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    17221 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    17281 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    17341 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    17401 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    17461 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    17521 aaaacgccag caacgcggcc tttttacggt tcctgtggtt ggcacataca aatggacgaa
    17581 cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc
    17641 ttag
//
