LOCUS       sequence-311416-        3574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden Gate vector, contains domesticated NAM-B1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: pNAM-B1
FEATURES             Location/Qualifiers
     source          1..3574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      408..465
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(489..506)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(660..1248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(740..759)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1431..1525)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1546..2205)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2120..2139
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2367..2385
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2389..2432)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ggcgccacct cgctgagcca cctagcagcg gcggcgaggg caagcccggc tccgaccaaa
       61 cagtttctcg ccccgtcatc gtcgaccccg ttcaactggc tcgatgcgtc accagtcggc
      121 atcctcccac aggcaaggaa ttttcctggg tttaacagga gcagaaacgt cggcaatatg
      181 tcgctgtcat cgacggccga catggctggc gcagtggaca acggtggagg caatgcggtg
      241 aacgccatgt ctacctatct tcccgtgcaa gacgggacgt accatcagca gcatgtcatc
      301 ctcggcgctc cgctggtgcc agaagccgcc gccgccacct ctggattcca gcatcccgtt
      361 caaatatccg gcgtgaactg gaatccctga gcttcgagac cctgcagtcc ggcaaaaaag
      421 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
      481 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      661 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1381 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     1441 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1501 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1561 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1621 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1681 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1741 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     1801 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1861 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1921 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1981 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2041 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2101 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2161 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2221 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2281 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
     2341 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     2401 aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcaaat gggcagctcc
     2461 gactcatctt ccggctcggc gcaaaaagca acgcggtatc accatcagca tcagccgccg
     2521 cctccgcagc ggggctcggc gccggagctc ccgccgggct tccggttcca cccgacggac
     2581 gaggagctgg tggtgcacta cctcaagaag aaggccgaca aggcgccgct ccccgtcaac
     2641 atcatcgccg aggtggatct ctacaagttc gacccatggg agctccccgg tatgttatgt
     2701 ctatctcgtc ggccggccgt gcttacttta tcaagcgccg caaattttag gtgcaattaa
     2761 ataatcgaat aatccatcca tctcatgctt atactcctgt gcacaagtag tatttttata
     2821 ttcttccagt acacatgtgt gtagatggtt tatgtatgtg atcctgtcgt gcttgttcat
     2881 gcgctcggga tccggatcca tcagagaagg cgaccatcgg ggagcaggag tggtacttct
     2941 tcagcccgcg cgaccgcaag taccccaacg gcgcgcggcc gaaccgggcg gcgacgtcgg
     3001 ggtactggaa ggccaccggc acggacaagc ctatcctggc ctcggggacg gggtgcggcc
     3061 tggtccggga gaagctcggc gtcaagaagg cgctcgtgtt ctaccgcggg aagccgccca
     3121 agggcctcaa aaccaactgg atcatgcacg agtaccgcct caccgacgca tctggctcca
     3181 ccaccgccac caaccgaccg ccgccggtga ccggcgggag cagggctgct gcctctctca
     3241 gggtacgtac acgtgtcgat cgcacggtct agcagtattt aattgctctc cagcttaatt
     3301 agggtattgt tgatggttga tgaagttgga cgactgggtg ctgtgccgca tctacaagaa
     3361 gatcaacaag gccgcggccg gcgatcagca gaggaacacg gagtgcgagg actccgtgga
     3421 ggacgcggtc accgcgtacc cgctctatgc cacggcgggc atgaccggtg caggtgcgca
     3481 tggcagcaac tacgcttcac cttcactgct ccatcatcag gacagccatt tcctggacgg
     3541 cctgttcaca gcagacgacg ccggcctctc ggcg
//
