LOCUS       sequence-311575-       14099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14099)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 14099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-CDK12-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..14099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1950..1970
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1970..3181
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3018..3037
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3182..3206
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7674..7698)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7700..7741
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7748..8704
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8710..8730)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8759..9347
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8812..8832)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9230..9241)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9361..9860
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9396..9419)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9793..9812
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9881..10480
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9881..9900)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10377..10397
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10618..10851
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10923..11044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10960..10979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11014..11033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11084..11219
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11239..11258)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11240..11258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11268..11285)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11268..11284)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11277..11299)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11426..11881
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11513..11532)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11723..11744
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11907..12011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12012..12080,12081..12872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13043..13631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13532..13551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13785..13802
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13919..13940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13955..13960,13961..13978,13979..13985)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13993..14009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13998..14020
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14017..14033
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14017..14033
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14052..14072
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14054..14072
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgtccagtgt ggtggaattc tgcagatatc aactttgtat agaaaagttg gcctccgcgc
     1981 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     2041 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     2101 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     2161 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     2221 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     2281 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     2341 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     2401 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     2461 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     2521 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     2581 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     2641 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     2701 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     2761 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     2821 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     2881 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     2941 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     3001 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     3061 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     3121 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     3181 cgcaactttg tacaaaaaag caggcttagc caccatgccc aattcagaga gacatggggg
     3241 caagaaggac gggagtggag gagcttctgg aactttgcag ccgtcatcgg gaggcggcag
     3301 ctctaacagc agagagcgtc accgcttggt atcgaagcac aagcggcata agtccaaaca
     3361 ctccaaagac atggggttgg tgacccccga agcagcatcc ctgggcacag ttatcaaacc
     3421 tttggtggag tatgatgata tcagctctga ttccgacacc ttctccgatg acatggcctt
     3481 caaactagac cgaagggaga acgacgaacg tcgtggatca gatcggagcg accgcctgca
     3541 caaacatcgt caccaccagc acaggcgttc ccgggactta ctaaaagcta aacagaccga
     3601 aaaagaaaaa agccaagaag tctccagcaa gtcgggatcg atgaaggacc ggatatcggg
     3661 aagttcaaag cgttcgaatg aggagactga tgactatggg aaggcgcagg tagccaaaag
     3721 cagcagcaag gaatccaggt catccaagct ccacaaggag aagaccagga aagaacggga
     3781 gctgaagtct gggcacaaag accggagtaa aagtcatcga aaaagggaaa cacccaaaag
     3841 ttacaaaaca gtggacagcc caaaacggag atccaggagc ccccacagga agtggtctga
     3901 cagctccaaa caagatgata gcccctcggg agcttcttat ggccaagatt atgaccttag
     3961 tccctcacga tctcatacct cgagcaatta tgactcctac aagaaaagtc ctggaagtac
     4021 ctcgagaagg cagtcggtca gtccccctta caaggagcct tcggcctacc agtccagcac
     4081 ccggtcaccg agcccctaca gtaggcgaca gagatctgtc agtccctata gcaggagacg
     4141 gtcgtccagc tacgaaagaa gtggctctta cagcgggcga tcacccagtc cctatggtcg
     4201 aaggcggtcc agcagccctt tcctgagcaa gcggtctctg agtcggagtc cactccccag
     4261 taggaaatcc atgaagtcca gaagtagaag tcctgcatat tcaagacatt catcttctca
     4321 tagtaaaaag aagagatcca gttcacgcag tcgtcattcc agtatctcac ctgtcaggct
     4381 tccacttaat tccagtctgg gagctgaact cagtaggaaa aagaaggaaa gagcagctgc
     4441 tgctgctgca gcaaagatgg atggaaagga gtccaagggt tcacctgtat ttttgcctag
     4501 aaaagagaac agttcagtag aggctaagga ttcaggtttg gagtctaaaa agttacccag
     4561 aagtgtaaaa ttggaaaaat ctgccccaga tactgaactg gtgaatgtaa cacatctaaa
     4621 cacagaggta aaaaattctt cagatacagg gaaagtaaag ttggatgaga actccgagaa
     4681 gcatcttgtt aaagatttga aagcacaggg aacaagagac tctaaaccca tagcactgaa
     4741 agaggagatt gttactccaa aggagacaga aacatcagaa aaggagaccc ctccacctct
     4801 tcccacaatt gcttctcccc caccccctct accaactact acccctccac ctcagacacc
     4861 ccctttgcca cctttgcctc caataccagc tcttccacag caaccacctc tgcctccttc
     4921 tcagccagca tttagtcagg ttcctgcttc cagtacttca actttgcccc cttctactca
     4981 ctcaaagaca tctgctgtgt cctctcaggc aaattctcag ccccctgtac aggtttctgt
     5041 gaagactcaa gtatctgtaa cagctgctat tccacacctg aaaacttcaa cgttgcctcc
     5101 tttgcccctc ccacccttat tacctggaga tgatgacatg gatagtccaa aagaaactct
     5161 tccttcaaaa cctgtgaaga aagagaagga acagaggaca cgtcacttac tcacagacct
     5221 tcctctccct ccagagctcc ctggtggaga tctgtctccc ccagactctc cagaaccaaa
     5281 ggcaatcaca ccacctcagc aaccatataa aaagagacca aaaatttgtt gtcctcgtta
     5341 tggagaaaga agacaaacag aaagcgactg ggggaaacgc tgtgtggaca agtttgacat
     5401 tattgggatt attggagaag gaacctatgg ccaagtatat aaagccaagg acaaagacac
     5461 aggagaacta gtggctctga agaaggtgag actagacaat gagaaagagg gcttcccaat
     5521 cacagccatt cgtgaaatca aaatccttcg tcagttaatc caccgaagtg ttgttaacat
     5581 gaaggaaatt gtcacagata aacaagatgc actggatttc aagaaggaca aaggtgcctt
     5641 ttaccttgta tttgagtata tggaccatga cttaatggga ctgctagaat ctggtttggt
     5701 gcacttttct gaggaccata tcaagtcgtt catgaaacag ctaatggaag gattggaata
     5761 ctgtcacaaa aagaatttcc tgcatcggga tattaagtgt tctaacattt tgctgaataa
     5821 cagtgggcaa atcaaactag cagattttgg acttgctcgg ctctataact ctgaagagag
     5881 tcgcccttac acaaacaaag tcattacttt gtggtaccga cctccagaac tactgctagg
     5941 agaggaacgt tacacaccag ccatagatgt ttggagctgt ggatgtattc ttggggaact
     6001 attcacaaag aagcctattt ttcaagccaa tctggaactg gctcagctag aactgatcag
     6061 ccgactttgt ggtagccctt gtccagctgt gtggcctgat gttatcaaac tgccctactt
     6121 caacaccatg aaaccgaaga agcaatatcg aaggcgtcta cgagaagaat tctctttcat
     6181 tccttctgca gcacttgatt tattggacca catgctgaca ctagatccta gtaagcggtg
     6241 cacagctgaa cagaccctac agagcgactt ccttaaagat gtcgaactca gcaaaatggc
     6301 tcctccagac ctcccccact ggcaggattg ccatgagttg tggagtaaga aacggcgacg
     6361 tcagcgacaa agtggtgttg tagtcgaaga gccacctcca tccaaaactt ctcgaaaaga
     6421 aactacctca gggacaagta ctgagcctgt gaagaacagc agcccagcac cacctcagcc
     6481 tgctcctggc aaggtggagt ctggggctgg ggatgcaata ggccttgctg acatcacaca
     6541 acagctgaat caaagtgaat tggcagtgtt attaaacctg ctgcagagcc aaaccgacct
     6601 gagcatccct caaatggcac agctgcttaa catccactcc aacccagaga tgcagcagca
     6661 gctggaagcc ctgaaccaat ccatcagtgc cctgacggaa gctacttccc agcagcagga
     6721 ctcagagacc atggccccag aggagtcttt gaaggaagca ccctctgccc cagtgatcct
     6781 gccttcagca gaacagatga cccttgaagc ttcaagcaca ccagctgaca tgcagaatat
     6841 attggcagtt ctcttgagtc agctgatgaa aacccaagag ccagcaggca gtctggagga
     6901 aaacaacagt gacaagaaca gtgggccaca ggggccccga agaactccca caatgccaca
     6961 ggaggaggca gcagagaaga ggccccctga gccccccgga cctccaccgc cgccacctcc
     7021 accccctctg gttgaaggcg atctttccag cgccccccag gagttgaacc cagccgtgac
     7081 agccgccttg ctgcaacttt tatcccagcc tgaagcagag cctcctggcc acctgccaca
     7141 tgagcaccag gccttgagac caatggagta ctccacccga ccccgtccaa acaggactta
     7201 tggaaacact gatgggcctg aaacagggtt cagtgccatt gacactgatg aacgaaactc
     7261 tggtccagcc ttgacagaat ccttggtcca gaccctggtg aagaacagga ccttctcagg
     7321 ctctctgagc caccttgggg agtccagcag ttaccagggc acagggtcag tgcagtttcc
     7381 aggggaccag gacctccgtt ttgccagggt ccccttagcg ttacacccgg tggtcgggca
     7441 accattcctg aaggctgagg gaagcagcaa ttctgtggta catgcagaga ccaaattgca
     7501 aaactatggg gagctggggc caggaaccac tggggccagc agctcaggag caggccttca
     7561 ctgggggggc ccaactcagt cttctgctta tggaaaactc tatcgggggc ctacaagagt
     7621 cccaccaaga gggggaagag ggagaggagt tccttactca ggctcgagcg gcaacccagc
     7681 tttcttgtac aaagtgggcg gcaagcccat ccccaacccc ctgctgggcc tggacagcac
     7741 cgctagcaaa gacaatactg tgcctctgaa gctgatcgct ctcctggcta atggcgagtt
     7801 ccatagtggc gaacagctgg gagaaaccct gggcatgtcc agggccgcta tcaacaagca
     7861 cattcagact ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa agggctactc
     7921 tctgcccgag cctatcccgc tgctgaacgc taaacagatt ctgggacagc tggacggcgg
     7981 gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg atcgaatcgg
     8041 cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag
     8101 agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct
     8161 gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc
     8221 gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca
     8281 ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca
     8341 gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa
     8401 tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc
     8461 tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc
     8521 atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat
     8581 tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct
     8641 ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga
     8701 aaagtaagcc aactttatta tacatagttg atatccagca cagtggcggc cgctcgacaa
     8761 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8821 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8881 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8941 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     9001 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     9061 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     9121 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     9181 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     9241 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     9301 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc
     9361 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     9421 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     9481 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     9541 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     9601 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     9661 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     9721 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     9781 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     9841 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     9901 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9961 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
    10021 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
    10081 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
    10141 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
    10201 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
    10261 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
    10321 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
    10381 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    10441 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
    10501 gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac
    10561 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
    10621 aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
    10681 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10741 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10801 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
    10861 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    10921 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10981 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    11041 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    11101 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    11161 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    11221 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    11281 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    11341 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    11401 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    11461 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    11521 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    11581 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    11641 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    11701 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11761 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    11821 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    11881 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    11941 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    12001 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    12061 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    12121 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    12181 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    12241 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    12301 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    12361 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    12421 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    12481 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    12541 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    12601 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    12661 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    12721 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    12781 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    12841 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    12901 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    12961 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    13021 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    13081 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    13141 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    13201 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    13261 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    13321 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    13381 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    13441 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    13501 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    13561 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    13621 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    13681 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    13741 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    13801 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    13861 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    13921 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    13981 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    14041 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaa
//
