LOCUS       sequence-31158-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Rossanese OW, Soderholm J, Bevis BJ, Sears IB, O'Connor J, 
            Williamson EK, Glick BS
  TITLE     Golgi structure correlates with transitional endoplasmic reticulum 
            organization in Pichia pastoris and Saccharomyces cerevisiae.
  JOURNAL   J Cell Biol. 1999 Apr 5. 145(1):69-81.
  PUBMED    10189369
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1999 Apr 5. 145(1):69-81."
COMMENT     Sequence Label: pUC19-URA3-3'SEC13-GFP
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(517..534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(517..533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(526..548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(742..761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     861..883
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc gtactgatga acttcaccag
       61 cgggttccca tttaccctca agattttcct tccataaagt aactttatta tcgccaccgg
      121 aaagagctag tacattacct gacaaagacc aactggctct ccataaaaca tctgggaatt
      181 tttcttcttt taataaagtt tttttccatg ggccttgttc attgtcttga gtccaaataa
      241 tacaggtgcg atcttgagaa acactggcca aataagaacg tagaagaaca gtaggtgacc
      301 atgctacgtc tctaacccaa tcgctgtgac cttctaaggt gctttccaga acataagttt
      361 gggcatctga attgtacttc caaatcttta ccaaattgtc agcaccccca gtaacaaact
      421 tgcgagattc tttagtaccg ttgtgttcac catcttcttc gatggtagct ggagcccaag
      481 aagcagagtt gatccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg
      541 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      601 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      661 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      721 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      781 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      841 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      901 gaaacgcgcg aga
//
