LOCUS       sequence-311600-       10532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a
            promoter for the auxin-inducible degron (AID) system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV-EF1amCherry-2A-OsTIR1-W
FEATURES             Location/Qualifiers
     source          1..10532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             522..702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    749..874
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1371..1604
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1789..1833
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2131..2248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2719..3900
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2950..3892
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3849..3869
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3920..4627
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4065..4083)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4333..4352
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4646..4699
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4700..6427
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(6493..6513)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6911..6922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7374..7391)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7380..7604
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7630..7650)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7630..7648)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7669..7685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7669..7685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7682..7704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7693..7709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7717..7723,7724..7741,7742..7747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7762..7783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7900..7917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8071..8659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8151..8170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8830..9621,9622..9690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9453..9472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9691..9795)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9821..10276)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9958..9979)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10170..10189
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10403..10425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10417..10434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10418..10434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10444..10463
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10444..10462
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
      541 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      601 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      661 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      721 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      781 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      841 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      901 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      961 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1021 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1081 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1141 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1201 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     1261 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1321 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1381 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1441 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1501 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1561 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1621 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1681 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1741 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1801 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1861 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1921 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1981 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2041 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     2101 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2161 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2221 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     2281 ttggttagta ccgggcccta cgcgtgaggg gcgcggaagc cggggcggag gaggagccaa
     2341 ccccgggctg ggctgagacc cgcagaggaa gacgctctag ggatttgtcc cggactagcg
     2401 agatggcaag gctgaggacg ggaggctgat tgagaggcga aggtacaccc taatctcaat
     2461 acaacctttg gagctaagcc agcaatggta gagggaagat tctgcacgtc ccttccaggc
     2521 ggcctccccg tcaccacccc ccccaacccg ccccgaccgg agctgagagt aattcataca
     2581 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta
     2641 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt
     2701 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2761 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaagtggcgc
     2821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2941 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3001 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
     3061 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3121 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3181 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3241 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3301 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3361 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3421 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3481 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3541 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3601 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     3661 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     3721 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3781 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3841 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     3901 gaagcttggc gcgcccacca tggtgagcaa gggcgaggag gataactccg ccatcatcaa
     3961 ggagttcctg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     4021 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     4081 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     4141 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     4201 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     4261 ggactcctct ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     4321 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     4381 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     4441 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     4501 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     4561 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     4621 gtacaagggc ggcgggtccg gaggagaggg cagaggaagt ctcctaacat gcggtgacgt
     4681 ggaggagaat cctggcccaa tgacatactt tcctgaagag gtcgtcgaac acatttttag
     4741 cttcctgcct gcacagagag atagaaacac agtgagcctg gtctgcaaag tgtggtacga
     4801 gatcgaacgc ctgagccgga gaggagtgtt cgtcggcaac tgctatgctg tgagagcagg
     4861 cagggtcgcc gctaggtttc caaatgtgcg cgcactgacc gtcaagggga aaccccactt
     4921 cgccgacttt aacctggtgc cccctgattg gggaggatac gccggccctt ggatcgaggc
     4981 agccgctcgc ggctgtcatg gactggagga actgcgcatg aagcgaatgg tggtctctga
     5041 cgaaagtctg gagctgctgg ctcggagctt ccctaggttt cgcgcactgg tgctgatttc
     5101 ttgcgaaggc ttcagcaccg atggactggc agccgtggcc tcccactgta agctgctgcg
     5161 ggagctggac ctccaggaga atgaagtgga ggatagaggc cccagatggc tgtcttgctt
     5221 cccagactca tgtaccagcc tggtgtccct gaactttgcc tgcatcaaag gcgaagtgaa
     5281 tgctgggtcc ctggagcggc tggtctcaag aagccccaac ctgaggtctc tgcggctgaa
     5341 ccggagcgtg agcgtggaca ctctggctaa gattctgctg agaaccccta acctggagga
     5401 tctgggaacc ggcaatctga cagacgattt ccagacagaa tcctacttta aactgacttc
     5461 tgccctggag aagtgtaaaa tgctgaggag tctgtcagga ttctgggatg cttcacccgt
     5521 gtgcctgagc tttatctacc ctctgtgtgc acagctgaca ggcctgaacc tgagctatgc
     5581 accaaccctg gacgccagtg atctgacaaa gatgatctca cgctgcgtga aactccagcg
     5641 actgtgggtg ctggactgta tttccgataa ggggctccag gtggtcgcca gctcctgcaa
     5701 ggacctccag gagctgagag tgttcccatc tgatttttac gtggccggat atagtgctgt
     5761 cactgaggaa ggcctggtgg cagtctcact gggatgccca aagctgaaca gcctgctgta
     5821 tttctgtcat cagatgacta atgctgcact ggtgaccgtc gccaagaact gccctaattt
     5881 cacccgattt cggctgtgta ttctggaacc aggcaaaccc gacgtggtca catcccagcc
     5941 actggatgaa gggtttggag ctatcgtgag agagtgcaag ggactccaga ggctgagcat
     6001 ttccggcctg ctgacagaca aagtgttcat gtacatcggc aagtatgcta agcagctgga
     6061 gatgctgagc attgcatttg ccggagactc cgataagggc atgatgcacg tgatgaacgg
     6121 gtgtaagaat ctgcgaaaac tggaaatccg ggacagccct ttcggggatg ccgctctgct
     6181 gggaaacttt gccagatacg agacaatgag gagcctgtgg atgtctagtt gcaatgtgac
     6241 tctgaagggc tgtcaggtcc tggctagtaa aatgcctatg ctgaacgtgg aagtcattaa
     6301 tgagcgggac gggtctaacg aaatggagga aaatcatggc gacctgccaa aggtggagaa
     6361 actgtatgtg tatcggacca ccgcaggggc aagagatgat gctcccaact ttgtgaagat
     6421 tctgtgagaa ttcgcggccg ctcaacctct ggattacaaa atttgtgaaa gattgactgg
     6481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7021 ctccccgcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7081 tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagtc aagatctgct
     7141 ttttgcctgt actgggtctc tctggttaga ccagagtctc tctggttaga ccagatctga
     7201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7321 agaccctttt agtcagtgtg gaaaatctct agcagtttaa acccgctgat cagcctcgac
     7381 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7441 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7501 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7561 ggaagtcaat agcaggcatg ctggggatgc ggtgggctct atgggcggcc gttaattaag
     7621 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     7681 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa
     7741 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7801 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7861 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7921 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7981 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8701 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8761 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8821 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8881 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8941 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9001 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9061 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9121 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9181 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9241 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9301 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9361 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9421 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9481 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9541 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9601 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9661 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9721 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9781 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     9841 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     9901 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     9961 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
    10021 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
    10081 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    10141 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
    10201 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
    10261 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10321 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    10381 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
    10441 gcgtaatacg actcactata gggcgaattg actagttatt aatagtaatc aattacgggg
    10501 tcattagttc atagcccata tatggagttc cg
//
