LOCUS       sequence-311643-       11690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Opto HOXD13 IDR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13-IDR-mCherry-CRY2
FEATURES             Location/Qualifiers
     source          1..11690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1590..3083
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    3235..3823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3288..3308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3706..3717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3826..3842)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3827..3843)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3933..4166
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4191..4209)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4192..4209)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4499..4518)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4732..4751)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4851..4873
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4911..4929)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4997..5101
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5102..5170,5171..5962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5320..5339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6133..6721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6622..6641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6875..6892
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6962..6982)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6967..7296
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7147..7282
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8430..8495
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8625..8645
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9070..9204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9107..9126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9161..9180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9372..10005
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10052..10177
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10674..10907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11092..11136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11421..11538
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccggagcta
      481 ccatgtcccg agccggtagc tgggacatgg acggcctgcg agcggacgga ggcggagctg
      541 gaggagctcc cgcatcaagt agtagctcaa gtgtggccgc tgccgcggct agcggacaat
      601 gtagggggtt tttgtcagcg cctgtctttg cgggcacaca ttccgggagg gccgctgctg
      661 cggctgctgc tgctgctgca gccgcggcgg ccgctagtgg atttgcctat cctgggacga
      721 gtgagcgcac tggttcatca tcctcatctt catccagtgc ggtagtcgcc gctcggccag
      781 aagcaccacc tgcaaaagag tgtccggccc cgacgccagc tgcagcagca gcggcaccgc
      841 cttcagctcc tgcgggtacc ggaggcggga tggtttccaa aggagaggag gataatatgg
      901 ctataattaa agagtttatg cggttcaagg tgcatatgga gggatctgtg aacggtcacg
      961 agttcgaaat cgagggcgaa ggcgaggggc gaccctatga agggacacag acggccaaac
     1021 tgaaggtgac caagggtggc cccctgcctt tcgcctggga catcctgtca ccccagttta
     1081 tgtacggctc taaggcttac gttaagcacc ctgccgatat tccggactac ttgaaactga
     1141 gcttccctga agggttcaag tgggaacggg taatgaattt tgaggatggc ggtgtagtca
     1201 cagttaccca ggacagctct cttcaggacg gagaatttat ctataaggtt aaacttcggg
     1261 gcactaactt cccatcagac ggccccgtga tgcagaagaa aactatgggc tgggaagcca
     1321 gctcagaacg catgtatccc gaggacggag cactgaaggg agaaatcaag cagcgactca
     1381 agctgaagga tgggggacat tatgatgctg aggtcaaaac cacctataag gccaagaaac
     1441 ccgttcagct tcctggggca tacaacgtga atatcaaact ggatattaca tcccataacg
     1501 aagactatac catcgtggaa cagtacgagc gggccgaggg cagacatagc acagggggca
     1561 tggatgaatt gtacaaggcg gccacgcgta tgaagatgga caaaaagact atagtttggt
     1621 ttagaagaga cctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat
     1681 ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag
     1741 cttcaagatg gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg
     1801 gatctgacct cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc
     1861 gcgttaccgg tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc
     1921 gggaccatac cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg
     1981 gagatctatt gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt
     2041 tcaattctta ctggaagaaa tgcttagata tgtcgattga atccgttatg cttcctcctc
     2101 cttggcggtt gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag
     2161 aactagggct ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttggt
     2221 ctccaggatg gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag
     2281 attatgcaaa gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc
     2341 tccatttcgg ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta
     2401 tatgggcaag agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa
     2461 tcggtttaag agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat
     2521 cgttgttgag tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct
     2581 ggagacaagg caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta
     2641 ccggatggat gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc
     2701 tccttccatg gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg
     2761 aatgtgacat ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg
     2821 atcgcttgga caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa
     2881 ggcaatggct tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg
     2941 ctcctttaac cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca
     3001 ttgtagacat cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag
     3061 cacagatcat gatcggagca gcagcccggg atccaccggt cgccaccggt tcagggagcg
     3121 gatccggctc agggagtgcg gccggaactc ccacctgcaa catgcgtgac tgactgaggc
     3181 cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
     3241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3601 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     3841 cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     3901 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3961 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4021 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4081 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4141 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     4201 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     4261 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     4321 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     4381 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     4441 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     4501 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     4561 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     4621 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     4681 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     4741 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4801 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4861 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4921 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4981 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5041 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5221 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5281 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5401 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5461 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5521 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5641 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggaa cacttctgcg
     5761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5941 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6241 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6781 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6841 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6901 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6961 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7021 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7141 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7201 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7261 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     7321 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     7381 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     7441 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     7501 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     7561 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     7621 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     7681 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     7741 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     7801 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     7861 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     7921 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     7981 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     8041 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     8101 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     8161 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     8221 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     8281 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     8341 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     8401 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     8461 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     8521 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     8581 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     8641 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     8701 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     8761 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     8821 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     8881 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     8941 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     9001 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     9061 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9181 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     9241 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     9301 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     9361 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     9421 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     9481 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     9541 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     9601 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     9661 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     9721 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     9781 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     9841 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9901 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9961 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    10021 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
    10081 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    10141 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    10201 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    10261 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    10321 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10381 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10441 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10501 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    10561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    10621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    10681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    10741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    10801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    10861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    10921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    10981 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    11041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    11101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    11161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    11221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    11281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    11341 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa
    11401 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
    11461 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
    11521 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat
    11581 aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt agtctccaga
    11641 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca
//
