LOCUS       sequence-311661-        6207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV production plasmid encoding for near-infrared calcium indicator 
            NIR-GECO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Qian Y, Cosio DMO, Piatkevich KD, Aufmkolk S, Su WC, Celiker OT, 
            Schohl A, Murdock MH, Aggarwal A, Chang YF, Wiseman PW, Ruthazer ES,
            Boyden ES, Campbell RE
  TITLE     Improved genetically encoded near-infrared fluorescent calcium ion 
            indicators for in vivo imaging.
  JOURNAL   PLoS Biol. 2020 Nov 24;18(11):e3000965. doi: 
            10.1371/journal.pbio.3000965. eCollection 2020 Nov.
  PUBMED    33232322
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-11-24"; volume: "18(11):e3000965. doi"; pages: "
            10.1371/journal.pbio.3000965. eCollection 2020 Nov"
COMMENT     Sequence Label: pAAV-CAG-NIR-GECO2
FEATURES             Location/Qualifiers
     source          1..6207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..278
                     /label=chicken beta-actin promoter
     primer_bind     557..579
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     641..660
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    2211..2799
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2264..2284)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2682..2693)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2842..2963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2853..2872)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2907..2926
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3125..3254
                     /label=AAV2 ITR
     rep_origin      complement(3491..4079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3571..3590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4250..5041,5042..5110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4873..4892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5111..5215)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5241..5696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5378..5399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5590..5609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5768..5897
                     /label=AAV2 ITR
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 ggcgcgcgcc gggggggggg ggggggggsg gggggsgggg sggggsgrgg cggagaggtg
      181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
      241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
      301 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
      481 gccctttgtg cggggggagc ggctcggggc tgtccgcggg gggacggctg ccttcggggg
      541 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
      601 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
      661 ctgtctcatc attttggcaa agaattggat ccgccaccat gtcggtaccg ctgactacct
      721 cagcattcgg ccacgcgttt ctggctaact gtgaacgtga gcagatccac ctggcgggct
      781 ccattcagcc gcacggtatc ctgctggctg ttaaagagcc tgacaacgtg gtgatccagg
      841 cttctattaa cgctgcggag ttcctgaaca ccaactttgt tgttggccgt ccgctgcgtg
      901 acctgggcgg cgatctgcct ttgcagatcc tgccgcacct gaacggcccg ctgcacctgg
      961 ctccgatgac cctgcgttgt actgtgggtt ctccgccgcg tcgtgtggac tgtaccattc
     1021 atcgaccgtc taacggcggc ctgatcgtag aactggaacc agcaaccaag gccactaaca
     1081 ttgcgccggc tctggtcggt gcgcttcatc gtatcacttc ttcatcctcc ctgatgggcc
     1141 tgtgtgacga aaccgcgact attttccgtg agattactgg tttcgaccgt gtgatggtaa
     1201 tgcgtctcgg cgcgcttgac gatctgactg aagagcagat cgcagagatt aaagaggctt
     1261 tctccctatt tgacaaggac ggggacggga cgataacaac caaggagctg gggacggtgt
     1321 tccggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc aatgaagtag
     1381 atgccgatgg cgacggcata ttcgacttcc ctgagttcct gacgatgatg gcaaggaaaa
     1441 tgaatgacac agacagtgaa gaggaaatta gaggagcgtt ccgcgtgttt gataaggacg
     1501 gcaatggcta catcggcgca gcagagcttc gccacgtgat gacagacctt ggtgagaagt
     1561 taactgatga ggaggttgat gaaatgatca gggtagcaga caacgatggg gatggtcagg
     1621 taaactacga agagtttgta caaatgatga cagcgaaggg tggcggaggt tctgtagatt
     1681 catcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt cggctgagct
     1741 cgcgtaggca tgatttgctg tccgaatgtc gtcgtgcgga cctggaagcg ttcctaggta
     1801 accgctaccc ggcgtctact attccgcaga tcgctcgtcg cctgtacgaa ctcaaccgtg
     1861 ttcgcctgct ggtagatgtg aactatactc cggttccgct agagccgcgc atcagcccgc
     1921 tgaacggtcg tgatctggat atgtccctgt cttgcctgcg ctctatgtcc ccgatccacc
     1981 agaaatacat gcaggacatg ggcgttggcg caaccctggt ttgctctctg atggtgtctg
     2041 gtcgtctgtg gggtctgatc gtttgccacc actacgaacc gcgctacgtt ccgtcccaca
     2101 ttcgcgctgc tggcgaagcg ctggcggaag catgtgcgaa ccgcatcgcg acgctggaga
     2161 gctttgcaca gtctcagtcc aaatgagaat tcgatatcaa gcttatcgat aatcaacctc
     2221 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2281 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2341 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2401 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2461 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2521 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2581 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2641 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2701 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2761 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacccgggc
     2821 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     2881 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2941 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3001 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     3061 ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt aatcattaac
     3121 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     3181 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     3241 gagcgagcgc gcagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3301 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3361 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3661 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3841 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3901 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3961 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4021 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4141 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4201 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4261 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4321 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4381 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4441 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4501 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4561 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4621 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4681 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4741 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4801 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4861 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4921 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4981 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5041 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5101 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5221 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     5281 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     5341 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5401 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5461 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     5521 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     5581 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     5641 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     5701 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5761 acgccagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc
     5821 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
     5881 catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc
     5941 atgctctagg aagagtacca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     6001 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     6061 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     6121 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     6181 acgtattagt catcgctatt accatgg
//
