LOCUS       sequence-311803-       14361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS 
            mutant construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 14361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pSLIK 3xFLAG-MAVS-Ala271 hygro
FEATURES             Location/Qualifiers
     source          1..14361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    163..280
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    337..361
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        393..707
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    401..419
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    437..455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    472..490
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    508..526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    544..562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    579..597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    615..633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     680..704
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             716..781
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2446..2470)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2520..3731
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3568..3587
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3856..4473,4474..4479,4480..4515,4516..4518,4519..4554,
                     4555..4557,4558..4593,4594..4602)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4655..5228
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4822..4839)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5049..5068
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5287..6306
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5287..6306
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6772..7360
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6825..6845)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7243..7254)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7363..7379)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7364..7380)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7889..8069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8095..8112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8101..8325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8371..8799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8458..8477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8668..8689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8808..8828)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8813..9142
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8993..9128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9055..9074
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9190..9237
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9256..9630
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9760..9881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9797..9816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9851..9870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9930..9946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9930..9946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9943..9965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9954..9970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9978..9984,9985..10002,10003..10008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10023..10044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10161..10178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10332..10920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10412..10431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11091..11882,11883..11951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11714..11733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11952..12056)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12131..12150)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12322..12701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12703..12901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12919..13099
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13146..13271
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13764..13997
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14182..14226
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
       61 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
      121 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
      181 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      241 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      301 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc
      361 tttaaaggaa ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa
      421 cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca
      481 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      541 tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg
      601 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt
      661 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgccact agatggacta
      721 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
      781 gactagtatg ccgtttgctg aagacaagac ctataagtat atctgccgca atttcagcaa
      841 tttttgcaat gtggatgttg tagagattct gccttacctg ccctgcctca cagcaagaga
      901 ccaggatcga ctgcgggcca cctgcacact ctcagggaac cgggacaccc tctggcatct
      961 cttcaatacc cttcagcggc ggcccggctg ggtggagtac ttcattgcgg cactgagggg
     1021 ctgtgagcta gttgatctcg cggacgaagt ggcctctgtc tacgagagct accagcctcg
     1081 gacctcggac cgtcccccag acccactgga gccaccgtca cttcctgctg agaggccagg
     1141 gccccccaca cctgctgcgg cccacagcat cccctacaac agctgcagag agaaggagcc
     1201 aagttacccc atgcctgtcc aggagaccca ggcgccagag tccccaggag agaattcaga
     1261 gcaagccctg cagacgctca gccccagagc catcccaagg aatccagatg gtggccccct
     1321 ggagtcctcc tctgacctgg cagccctcag ccctctgacc tccagcgggc atcaggagaa
     1381 ggacacagaa ctgggcagta cccacacagc aggtgcgacc tccagcctca caccatcccg
     1441 tgggcctgtg tctccatctg tctccttcca gcccctggcc cgttccaccc ccagggcaag
     1501 ccgcttgcct ggacccacag ggtcagttgt atctactggc acctccttct cctcctcatc
     1561 ccctggcttg gcctctgcag gggctgcaga gggtaaagcg ggtgcagaga gtgaccaggc
     1621 cgagcctatc atctgctcca gtggggcaga ggcacctgcc aactctctgc cctccaaagt
     1681 gcctaccacc ttgatgcctg tgaacacagt ggccctgaaa gtgcctgcca acccagcatc
     1741 tgtcagcaca gtgccctcca agttgccaac tagctcaaag ccccctggtg cagtgccttc
     1801 taatgcgctc accaatccag caccatccaa attgcccatc aactcaaccc gtgctggcat
     1861 ggtgccatcc aaagtgccta ctagcatggt gctcaccaag gtgtctgcca gcacagtccc
     1921 cactgacggg agcagcagaa atgaggagac cccagcagct ccaacacccg ccggcgccac
     1981 tggaggcagc tcagcctggc tagacagcag ctttgagaat aggggccttg ggtcggagct
     2041 gagtaagcct agcgtgctgg catcccaggt agacagcccg ttctcgggct gcttcgagga
     2101 tcttgccatc agtgccagca cctccttggg catggggccc tgccatggcc cagaggagaa
     2161 tgagtataag tccgagggca cctttgggat ccacgtggct gagaacccca gcatccagct
     2221 cctggagggc aaccctgggc cacctgcgga cccggatggc ggccccaggc cacaagccga
     2281 ccggaagttc caggagaggg aggtgccatg ccacaggccc tcacctgggg ctctgtggct
     2341 ccaggtggct gtgacagggg tgctggtagt cacactcctg gtggtgctgt accggcggcg
     2401 tctgcactag caattggaag actaatgcgt tttcgagata tctagaccca gctttcttgt
     2461 acaaagtggt tgataacatc tcgagccata aagatggtta attaacccac ccaagatctg
     2521 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
     2581 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
     2641 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
     2701 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
     2761 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
     2821 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
     2881 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
     2941 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
     3001 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
     3061 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
     3121 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc
     3181 gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg
     3241 acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt
     3301 atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg
     3361 tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc
     3421 ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc
     3481 gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg
     3541 ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt
     3601 tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa
     3661 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
     3721 ttttgttaga cgaagcttgg taccgagctc ggatctccac cccgtaccgg tcctgcagga
     3781 tttaaatggc gcgccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
     3841 gcagtcgaat tcaccatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     3901 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     3961 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     4021 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     4081 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     4141 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     4201 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     4261 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     4321 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     4381 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     4441 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     4501 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     4561 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatccgcgg
     4621 ccgcactaga ggaattccgc ccctctcccc cccccccctc tcccyccccc ccccctaacg
     4681 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4741 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4801 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4861 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4921 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4981 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     5041 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     5101 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     5161 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5221 gatgataagc ttaccggtac gcgtgatagc ttgccacaac ccgtaccaaa gatggataga
     5281 tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     5341 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     5401 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     5461 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     5521 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     5581 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     5641 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     5701 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     5761 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     5821 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     5881 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     5941 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     6001 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     6061 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     6121 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     6181 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     6241 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     6301 gaatagagta gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc
     6361 gcgcgagcct agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg
     6421 tccacagttc gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg
     6481 cccttctgga ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc
     6541 tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatctcta
     6601 gatctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     6661 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     6721 atgcaattgt tgttgtttag tccctcccaa ttcgatatca agcttatcga taatcaacct
     6781 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6841 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6901 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6961 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7021 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7081 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7141 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7201 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7261 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7321 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     7381 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     7441 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     7501 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7561 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     7621 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     7681 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     7741 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     7801 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     7861 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     7921 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7981 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8041 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     8101 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     8161 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8221 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     8281 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     8341 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     8401 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8461 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8521 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8581 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8641 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8701 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8761 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     8821 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8881 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8941 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9001 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9061 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     9121 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     9181 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     9241 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     9301 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     9361 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     9421 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     9481 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     9541 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     9601 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     9661 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     9721 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     9781 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9841 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     9901 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9961 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    10021 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10081 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    10141 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    10201 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    10261 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    10321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    10381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    10441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    10501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    10561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    10621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10741 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    10801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    11041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    11101 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    11161 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    11221 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    11281 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    11341 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    11401 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    11461 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    11521 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    11581 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    11641 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11701 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11761 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11821 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11881 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11941 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    12001 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    12061 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
    12121 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
    12181 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
    12241 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
    12301 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
    12361 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
    12421 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
    12481 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
    12541 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
    12601 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
    12661 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
    12721 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
    12781 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
    12841 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
    12901 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    12961 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    13021 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    13081 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
    13141 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
    13201 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
    13261 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
    13321 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
    13381 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
    13441 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
    13501 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
    13561 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
    13621 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
    13681 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    13741 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    13801 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    13861 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    13921 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    13981 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    14041 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    14101 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    14161 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    14221 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    14281 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    14341 gttaggcagg gatattcacc a
//
