LOCUS       sequence-311807-       14361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS 
            mutant construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 14361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro
FEATURES             Location/Qualifiers
     source          1..14361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    195..312
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    369..393
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        425..739
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    433..451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    469..487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    504..522
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    540..558
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    576..594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    611..629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    647..665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     712..736
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             748..813
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2478..2502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2552..3763
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3600..3619
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3888..4505,4506..4511,4512..4547,4548..4550,4551..4586,
                     4587..4589,4590..4625,4626..4634)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4687..5260
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4854..4871)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5081..5100
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5319..6338
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5319..6338
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6804..7392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6857..6877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7275..7286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7395..7411)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7396..7412)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7921..8101
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8127..8144)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8133..8357
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8403..8831
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8490..8509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8700..8721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8840..8860)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8845..9174
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9025..9160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9087..9106
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9222..9269
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9288..9662
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9792..9913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9829..9848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9883..9902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9962..9978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9962..9978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9975..9997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9986..10002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10010..10016,10017..10034,10035..10040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10055..10076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10193..10210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10364..10952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10444..10463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11123..11914,11915..11983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11746..11765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11984..12088)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12163..12182)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12354..12733
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12735..12933
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12951..13131
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13178..13303
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13796..14029
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14214..14258
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
       61 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      121 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
      181 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      241 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      301 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt aattctagac
      361 agttatcaac aagtttgtac aaaaaagcag gctttaaagg aaccaattca gtcgactgga
      421 tcccgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      481 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
      541 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
      601 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      661 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      721 agtgaaccgt cagatcgcca ctagatggac tacaaagacc atgacggtga ttataaagat
      781 catgacatcg attacaagga tgacgatgac aagactagta tgccgtttgc tgaagacaag
      841 acctataagt atatctgccg caatttcagc aatttttgca atgtggatgt tgtagagatt
      901 ctgccttacc tgccctgcct cacagcaaga gaccaggatc gactgcgggc cacctgcaca
      961 ctctcaggga accgggacac cctctggcat ctcttcaata cccttcagcg gcggcccggc
     1021 tgggtggagt acttcattgc ggcactgagg ggctgtgagc tagttgatct cgcggacgaa
     1081 gtggcctctg tctacgagag ctaccagcct cggacctcgg accgtccccc agacccactg
     1141 gagccaccgt cacttcctgc tgagaggcca gggcccccca cacctgctgc ggcccacagc
     1201 atcccctaca acagctgcag agagaaggag ccaagttacc ccatgcctgt ccaggagacc
     1261 gcggcgccag agtccccagg agagaattca gagcaagccc tgcagacgct cagccccaga
     1321 gccatcccaa ggaatccaga tggtggcccc ctggagtcct cctctgacct ggcagccctc
     1381 agccctctga cctccagcgg gcatcaggag aaggacacag aactgggcag tacccacaca
     1441 gcaggtgcga cctccagcct cacaccatcc cgtgggcctg tgtctccatc tgtctccttc
     1501 cagcccctgg cccgttccac ccccagggca agccgcttgc ctggacccac agggtcagtt
     1561 gtatctactg gcacctcctt ctcctcctca tcccctggct tggcctctgc aggggctgca
     1621 gagggtaaag cgggtgcaga gagtgaccag gccgagccta tcatctgctc cagtggggca
     1681 gaggcacctg ccaactctct gccctccaaa gtgcctacca ccttgatgcc tgtgaacaca
     1741 gtggccctga aagtgcctgc caacccagca tctgtcagca cagtgccctc caagttgcca
     1801 actagctcaa agccccctgg tgcagtgcct tctaatgcgc tcaccaatcc agcaccatcc
     1861 aaattgccca tcaactcaac ccgtgctggc atggtgccat ccaaagtgcc tactagcatg
     1921 gtgctcacca aggtgtctgc cagcacagtc cccactgacg ggagcagcag aaatgaggag
     1981 accccagcag ctccaacacc cgccggcgcc actggaggca gctcagcctg gctagacagc
     2041 agctttgaga ataggggcct tgggtcggag ctgagtaagc ctggcgtgct ggcatcccag
     2101 gtagacagcc cgttctcggg ctgcttcgag gatcttgcca tcagtgccag cacctccttg
     2161 ggcatggggc cctgccatgg cccagaggag aatgagtata agtccgaggg cacctttggg
     2221 atccacgtgg ctgagaaccc cagcatccag ctcctggagg gcaaccctgg gccacctgcg
     2281 gacccggatg gcggccccag gccacaagcc gaccggaagt tccaggagag ggaggtgcca
     2341 tgccacaggc cctcacctgg ggctctgtgg ctccaggtgg ctgtgacagg ggtgctggta
     2401 gtcacactcc tggtggtgct gtaccggcgg cgtctgcact agcaattgga agactaatgc
     2461 gttttcgaga tatctagacc cagctttctt gtacaaagtg gttgataaca tctcgagcca
     2521 taaagatggt taattaaccc acccaagatc tggcctccgc gccgggtttt ggcgcctccc
     2581 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     2641 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     2701 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     2761 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     2821 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     2881 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     2941 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     3001 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     3061 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     3121 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     3181 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     3241 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     3301 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     3361 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     3421 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     3481 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     3541 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     3601 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     3661 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     3721 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt ggtaccgagc
     3781 tcggatctcc accccgtacc ggtcctgcag gatttaaatg gcgcgccgct agcgctaccg
     3841 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga attcaccatg tctagactgg
     3901 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     3961 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     4021 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     4081 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     4141 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     4201 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     4261 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     4321 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     4381 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     4441 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     4501 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     4561 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     4621 tgctccccgg gtaactaagt aaggatccgc ggccgcacta gaggaattcc gcccctctcc
     4681 cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     4741 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     4801 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     4861 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     4921 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     4981 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     5041 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     5101 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     5161 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     5221 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttaccggt acgcgtgata
     5281 gcttgccaca acccgtacca aagatggata gatccggaaa gcctgaactc accgcgacgt
     5341 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     5401 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     5461 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     5521 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     5581 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     5641 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     5701 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     5761 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     5821 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     5881 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     5941 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     6001 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     6061 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     6121 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     6181 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     6241 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     6301 accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga ccgaacaaga
     6361 gctgatttcg agaacgcctc agccagcaac tcgcgcgagc ctagcaaggc aaatgcgaga
     6421 gaacggcctt acgcttggtg gcacagttct cgtccacagt tcgctaagct cgctcggctg
     6481 ggtcgcggga gggccggtcg cagtgattca ggcccttctg gattgtgttg gtccccaggg
     6541 cacgattgtc atgcccacgc actcgggtga tctgactgat cccgcagatt ggagatcgcc
     6601 gcccgtgcct gccgattggg tgcagatctc tagatctgta caagtaaagc ggccgcgact
     6661 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6721 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgttt agtccctccc
     6781 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     6841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7201 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7321 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7381 ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc
     7441 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7501 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7561 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7621 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     7681 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     7741 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     7801 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     7861 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     7921 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7981 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8041 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8101 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     8161 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     8221 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     8281 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     8341 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     8401 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8461 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8521 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8581 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8641 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8701 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8761 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8821 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     8881 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     8941 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9001 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9061 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     9121 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9181 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     9241 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     9301 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     9361 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     9421 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     9481 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     9541 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     9601 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     9661 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     9721 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     9781 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9841 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9901 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     9961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10021 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10081 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10801 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    11761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    11821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    12121 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    12181 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    12241 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    12301 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
    12361 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    12421 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    12481 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    12541 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    12601 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    12661 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
    12721 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
    12781 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
    12841 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
    12901 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
    12961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    13021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    13081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    13141 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    13201 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    13261 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    13321 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    13381 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    13441 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    13501 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    13561 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    13621 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    13681 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    13741 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    13801 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    13861 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    13921 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    13981 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    14041 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    14101 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    14161 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    14221 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    14281 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    14341 tagtttttgc tgtactttct a
//
