LOCUS       sequence-31183-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-PCMT
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(847..865)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc aacaacatgt acaaactgtc tgagaacaac
      121 cataagtcct tactggagaa cctgaaacgt cgcggcatca tcgacgacga cgacgtgtac
      181 aacaccatgc tgcaagttga tcggggtaaa tatattaagg agatcccgta tatagacacg
      241 ccagtctaca ttagtcacgg ggtaactata tcagcgcctc atatgcacgc cttgtcgctt
      301 aaaagactca ttaatgtgtt aaaacccgga agccgagcaa tcgatgttgg ctctggtagt
      361 gggtatttga cagtctgcat ggctattaag atgaatgtac ttgaaaataa aaattcatac
      421 gtgataggcc tcgaacgtgt taaagatctg gtcaattttt ccttagagaa catcaagcgc
      481 gataaaccgg aactgttgaa aatcgacaat ttcaagatca tccacaaaaa catctaccaa
      541 gtgaacgagg aggagaaaaa ggagctgggt ctgtttgacg cgattcatgt aggcgcctcg
      601 gcaagcgaac ttccagaaat tctcgttgat ttattggctg aaaatggaaa acttattatt
      661 ccgatagagg aagattatac ccaggtgctg tatgaaatta cgaaaaagaa cggtaaaatc
      721 atcaaagacc gtctgttcga cgtctgtttc gtgtccctca agaaaaactg atgacgaagc
      781 ttgcggccgc actcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      841 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttga
//
