LOCUS       sequence-31184-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-FTSJ
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(771..789)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      785..832
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca attatagaag tagggcggca tataagttaa
      121 ttgaattgga taataaatat ttgttcttaa aaaaaaataa aataatttta gatattggat
      181 gttatccagg tagttggtgt caagttatat tagagcgtac taaaaattat aaaaataaaa
      241 ttataggaat agataagaag ataatggatc ctatacctaa cgtttatttt attcaagggg
      301 aaattggtaa agataatatg aacaatataa agaatataaa ttatattgat aatatgaata
      361 ataattctgt agattataaa ttaaaagaaa tattacaaga taaaaaaatt gacattatat
      421 taagtgacgc tgctgttcca tgtatcggaa acaaaattga tgatcattta aattcttgtg
      481 aacttacctt atcaataacc cattttatgg aacaatatat aaatataggt ggtacatata
      541 ttgttaaaat gtatttgggt agtcaaacca ataatttaaa aacatattta aaaggtatgt
      601 tccaactcgt tcatacaacc aaaccaaaag catcgagaaa tgaatcaagg gaaatatatc
      661 ttgtttgtaa aaattttttg ggaagaaaaa aatgatgacg aagcttgcgg ccgcactcga
      721 ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      781 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      841 aggacctata tccggatatc ccgcaggagg cccggcagta cnggcataac ccagcctatg
      901 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgc
//
