LOCUS       sequence-311915-       10222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Kyriakakis P, Fernandez de Cossio L, Howard PW, Kouv S, Catanho M, 
            Hu VJ, Kyriakakis R, Allen ME, Ma Y, Aguilar-Rivera M, Coleman TP
  TITLE     Building a Simple and Versatile Illumination System for Optogenetic 
            Experiments.
  JOURNAL   J Vis Exp. 2021 Jan 12;(167). doi: 10.3791/61914.
  PUBMED    33522514
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2021-01-12"; volume: "(167). doi"; pages: " 
            10.3791/61914"
COMMENT     Sequence Label: pPK-351
FEATURES             Location/Qualifiers
     source          1..10222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        193..572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     727..747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     821..840
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        821..839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2625..2636
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2652..2672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2685..2711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(2724..3299,3300..3302,3303..3311)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2892..2909)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3119..3138
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5199..5219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5229..5669
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5685..5711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(5733..5751)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5734..5751)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5771..5788)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5777..6001
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6047..6475
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6134..6153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6344..6365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6484..6504)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6489..6818
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6669..6804
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6731..6750
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6885..7679
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6939..6958)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7549..7568
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7853..7974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7890..7909)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7944..7963
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8023..8039)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8023..8039)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8036..8058)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8047..8063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8071..8077,8078..8095,8096..8101))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8116..8137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8254..8271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8425..9013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8505..8524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9184..9975,9976..10044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9807..9826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10045..10149)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
       61 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
      121 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
      181 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      241 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      301 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      361 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      421 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      481 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      541 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      601 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      661 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      721 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
      781 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
      841 agacccaagc ttgatccgcc gccaccatgc ctctgtttga gcttttcagg ctcaccaaag
      901 ctaagcttga atctgctcaa gacaggaacc cttctccacc tgtagatgaa gttgtggagc
      961 tggtgtggga aaatggtcag atatcaactc aaagtcagtc aagtagatcg aggaacattc
     1021 ctccaccaca agcaaactct tctagagcta gagagattgg aaatggctca aagacgacta
     1081 tggtggacga gatccctatg tcagtgccat cactaatgac gggtttgagt caagacgatg
     1141 actttgttcc atggttgaat catcatccct cccttgatgg atattgctct gatttcttgc
     1201 gtgatgtgtc gtctcctgtt actgtcaacg agcaagagag tgatatggcg gtaaaccaaa
     1261 ctgctttccc gttgtttcag agaagaaagg atggcaatga atcagctcct gctgcttctt
     1321 cgtcgcagta taacggtttc caatcgcatt ctctgtatgg aagtgataga gctagagatc
     1381 ttcctagcca acaaaccaat ccggatcggt ttactcagac gcaggaacca ctaattacta
     1441 gtaacaagcc tagtttggtc aacttttcac atttcttacg ccctgcaact tttgcgaaga
     1501 ctactaataa taaccttcat gacactaaag aaaagagtcc tcaaagcccg ccaaatgtgt
     1561 ttcagaccag agttcttgga gctaaagact ctgaagataa ggttcttaac gagtctgttg
     1621 cttctgctac gcctaaagat aaccaaaagg cttgcctaat atcagaggac tcatgtagaa
     1681 aagaccaaga gagtgaaaaa gcagttgtat gttcttctgt tggctcgggt aatagtctcg
     1741 atggcccatc cgaaagtcct tcactttctt taaagagaaa gcattcgaat attcaagaca
     1801 ttgactgtca tagtgaagat gtggaagaag aatcaggaga tggaagaaag gaagcaggtc
     1861 catctcgaac gggtttgggt tcaaagagaa gccgctctgc agaagtgcat aatctgtctg
     1921 aaaggagacg gcgtgatagg atcaacgaga agatgcgtgc cctgcaagaa ctcattccaa
     1981 actgtaacaa ggtggacaaa gcttcgatgc tagatgaagc catcgagtat ctcaagtcac
     2041 tccaacttca agtgcagatc atgtcaatgg cgtctggtta ctatctgcca ccggcggtta
     2101 tgttcccacc gggtatgggg cattacccgg cagcagctgc tgcaatggca atgggtatgg
     2161 gaatgcctta tgcaatgggc ttgcctgatt tgagccgtgg tggttcatcg gttaaccacg
     2221 gaccacagtt ccaagtctcg gggatgcaac aacaaccagt ggcgatgggt attccacgtg
     2281 tctctggtgg tggtatcttt gccggttctt cgacgattgg caatggctcg actagagatt
     2341 tatctggttc taaagatcaa acaacgacga ataacaacag taacttgaaa ccaataaaga
     2401 gaaaacaggg gtcttctgat cagttttgtg gatcgtggcg cgccatggga tctcctaggt
     2461 ttgatactga cctgttcgat accgacttgt ttgatactga cctttttgat actgacctgt
     2521 tcgataccga cttgtttgat actgaccttt ttgatactga cctgttcgat accgacttgt
     2581 ttgatactga cctttttgat actgacctgt tcgataccga cttgatcgag ggaaggggtg
     2641 gcgaattcat gcctaagaaa aagagaaagg tgggcggaat ggcttaccca tacgatgttc
     2701 cagattacgc ttgataacat atgcccctct ccctcccccc ccccctaacg ttactggccg
     2761 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     2821 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2881 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2941 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3001 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3061 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3121 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3181 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
     3241 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3301 tggccacaac cactagttcg cgaatggttt ccggagtcgg gggtagtggc ggtggccgtg
     3361 gcggtggccg tggcggagaa gaagaaccgt cgtcaagtca cactcctaat aaccgaagag
     3421 gaggagaaca agctcaatcg tcgggaacga aatctctcag accaagaagc aacactgaat
     3481 caatgagcaa agcaattcaa cagtacaccg tcgacgcaag actccacgcc gttttcgaac
     3541 aatccggcga atcagggaaa tcattcgact actcacaatc actcaaaacg acgacgtacg
     3601 gttcctctgt acctgagcaa cagatcacag cttatctctc tcgaatccag cgaggtggtt
     3661 acattcagcc tttcggatgt atgatcgccg tcgatgaatc cagtttccgg atcatcggtt
     3721 acagtgaaaa cgccagagaa atgttaggga ttatgcctca atctgttcct actcttgaga
     3781 aacctgagat tctagctatg ggaactgatg tgagatcttt gttcacttct tcgagctcga
     3841 ttctactcga gcgtgctttc gttgctcgag agattacctt gttaaatccg gtttggatac
     3901 attccaagaa tactggtaaa ccgttttacg ccattcttca taggattgat gttggtgttg
     3961 ttattgattt agagccagct agaactgaag atcctgcgct ttctattgct ggtgctgttc
     4021 aatcgcagaa actcgcggtt cgtgcgattt ctcagttaca ggctcttcct ggtggagata
     4081 ttaagctttt gtgtgacact gtcgtggaaa gtgtgaggga cttgactggt tatgatcgtg
     4141 ttatggttta taagtttcat gaagatgagc atggagaagt tgtagctgag agtaaacgag
     4201 acgatttaga gccttatatt ggactgcatt atcctgctac tgatattcct caagcgtcaa
     4261 ggttcttgtt taagcagaac cgtgtccgaa tgatagtaga ttgcaatgcc acacctgttc
     4321 ttgtggtcca ggacgatagg ctaactcagt ctatgtgctt ggttggttct actcttaggg
     4381 ctcctcatgg ttgtcactct cagtatatgg ctaacatggg atctattgcg tctttagcaa
     4441 tggcggttat aatcaatgga aatgaagatg atgggagcaa tgtagctagt ggaagaagct
     4501 cgatgaggct ttggggtttg gttgtttgcc atcacacttc ttctcgctgc ataccgtttc
     4561 cgctaaggta tgcttgtgag tttttgatgc aggctttcgg tttacagtta aacatggaat
     4621 tgcagttagc tttgcaaatg tcagagaaac gcgttttgag aacgcagaca ctgttatgtg
     4681 atatgcttct gcgtgactcg cctgctggaa ttgttacaca gagtcccagt atcatggact
     4741 tagtgaaatg tgacggtgca gcatttcttt accacgggaa gtattacccg ttgggtgttg
     4801 ctcctagtga agttcagata aaagatgttg tggagtggtt gcttgcgaat catgcggatt
     4861 caaccggatt aagcactgat agtttaggcg atgcggggta tcccggtgca gctgcgttag
     4921 gggatgctgt gtgcggtatg gcagttgcat atatcacaaa aagagacttt cttttttggt
     4981 ttcgatctca cactgcgaaa gaaatcaaat ggggaggcgc taagcatcat ccggaggata
     5041 aagatgatgg gcaacgaatg catcctcgtt cgtcctttca ggcttttctt gaagttgtta
     5101 agagccggag tcagccatgg gaaactgcgg aaatggatgc gattcactcg ctccagctta
     5161 ttctgagaga ctcttttaaa gaatctttgc ggccgcaacc taagaaaaag agaaaggtgg
     5221 gcggaggcat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa
     5281 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt
     5341 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg
     5401 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg
     5461 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg
     5521 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata
     5581 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta
     5641 acaaaggtca aagacagttg actgtatcgc cggaattcat ggcttaccca tacgatgttc
     5701 cagattacgc ttgataatct agagggccct attctatagt gtcacctaaa tgctagagct
     5761 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5821 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5881 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5941 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6001 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6061 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6121 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6181 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6241 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6301 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6361 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6421 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6481 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6541 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6661 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6721 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6781 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6841 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     6901 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     6961 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7021 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     7081 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7141 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7201 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7261 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7321 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7381 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     7441 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7501 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7561 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7621 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     7681 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     7741 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7801 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7861 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7921 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7981 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     8041 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8101 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8161 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8221 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8281 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8341 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8401 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8461 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8521 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8581 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8641 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8761 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8821 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8881 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8941 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9001 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9061 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9121 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9181 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9241 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9301 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9361 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9421 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9481 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9541 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9601 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9661 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9721 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9781 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9841 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9901 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9961 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10021 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10081 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10141 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
    10201 gatcccctat ggtgcactct ca
//
