LOCUS       sequence-31195-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-MECPS
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(728..746)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      742..789
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaaatatt ttgtttactt taagaaggag atataccatg catcatcatc atcatcacag
       61 cagcggcaga gaaaacttgt atttccaggg ctacgacggg gtgagaatag gccagggcta
      121 tgacattcac cagatacggg tgggcccccc ggaagacatc gtcgcggaca ccaccgcaga
      181 caccgccgcg aacaccgctg atcccaacaa gcagagcttc aaaaggctga ccatcggggg
      241 ggtgccagtg gagaccattt ccgtgctgtc gcacagtgat ggggatgtca ttttccacgc
      301 actagtggac gccctacttg gcggcatgag ttgttcggac ctgggaaccc tctttcctga
      361 tggctcacca aaatataaaa ataaaaactc actctccttt ttaagatatg ccagactgct
      421 tttgtataaa aggaattacg ccatcgcaaa tgtggacatc atcgtcatcg cggaagttcc
      481 caagataagt cccattcgcg aggaaattgt tcgcaacata tcttctgctc tgggcatctc
      541 ggaatcgcag gtctccctca aaggaaaaac tcacgagcag ctcggccccg tcggacagaa
      601 aaaggcaatc gagtgcttcg caaacgcgct cttaattcga aagcaatcat gatgacgaag
      661 cttgcggccg cactcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      721 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      781 ggttttttgc tgaaaggagg aactatatcc ggatatcccg caagaggccc ggcagtaccg
      841 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      901 cattgttaga tttcatacac ggtgcctgac tgcgtta
//
