LOCUS       sequence-311950-        9512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for mammalian overexpression of the mutated ER isoform of rat USP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  TITLE     rat USP19 constructs
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HisFlag-rUSP19ER-CRmut
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        122..501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        502..705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     656..676
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     750..769
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        750..768
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      875..884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             887..904
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             905..970
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4988..5005)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4994..5218
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5264..5692
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5351..5370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5561..5582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5701..5721)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5706..6035
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5886..6021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5948..5967
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6102..6896
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6156..6175)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6766..6785
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7072..7193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7109..7128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7163..7182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7242..7258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7242..7258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7255..7277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7266..7282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7290..7296,7297..7314,7315..7320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7335..7356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7473..7490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7644..8232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7724..7743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8403..9194,9195..9263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9026..9045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9264..9368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9443..9462)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
       61 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      121 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      181 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      241 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      301 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      361 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      421 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      481 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      541 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      601 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      661 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
      721 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      781 ggctagttaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag
      841 atatccagca cagtggcggc cgctcgagtc tagagccacc atggcacatc atcatcatca
      901 tcacgactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
      961 cgatgacaag cgcccgggct gcaggaattc gatgggtcca tgtgctggca ccagtgctac
     1021 aggcccaagg agggggcctc cgggattgga ggaggcaact agtaagaaga aacagaagga
     1081 tcgagcaaac caggaaagta aggatggaga tcctaggaga gtgtccatgc ctcgaaagga
     1141 gcccaccaaa gatgaactgt tgctcgattg gagacagagt tcagataagg tggttgttaa
     1201 gctgcgcgtg ggaacaggtc ccatatgtct ggaggaagta gatgctgctt tcacagacac
     1261 ggactgtgtg gtgaggcttc cagatggtcg gcagtggggt ggtgtcttct ttgctgaaat
     1321 acaaagttct tgtaccaaag tgcagactca caagggtggt cttctacagt tggcactacc
     1381 aaagaaggtg cctcttctca cgtggccctc tctcctgaag aaacctctag ggacccaaga
     1441 actggtgcca ggattgcggt gccaggagaa cggacaagaa ctgtctccca ttgccctgga
     1501 gccaggttct gagccccgca gagctaaaca ggaagctcga aaccagaaga gggcccaggg
     1561 ccgtggtgag gtaggctcag gggctagccc tggggcacag gcagggccca gtgccaagag
     1621 ggctgttcat ctctgcagag ggccagaagg tgaagggtcc atggatgggc ctggccccca
     1681 gggtgatgcc ccatccttcc tgtccgactc agctacgcag gttgaggctg aggagcagct
     1741 ccatgttcca ccagtgaacc ctcaaacaag tctcttgggc tcagagaaga atttagccct
     1801 tttgacagta gagaagacag tgtcccccag gagtgactca gtctccccag tcatgatccg
     1861 gaacagagac cctgagaaag atgaccattt caaagaggag atggcagttg gagctgatcc
     1921 tgcagcctta gcggatgaac cagagtctat ggtgaacctg gcatttgtca agaatgattc
     1981 gtatgagaag gggccggatt cagtggtggt gcacgtgtat gtgaaggaga gccgcaggga
     2041 tacctctcga gtacttttcc gagagcagga cttcacgctg atcttccaga ccagggatgg
     2101 aaattttctg aggctgcatc cgggctgtgg gccccacacc atcttccgct ggcaggtgaa
     2161 gctcaggaac ttgattgagc cagagcaatg tacgttttgt ttcacggcct ctcgaatcga
     2221 tatctgcctc cggaagcggc agagtcagcg ctggggggga ctggaggccc ctgctacacg
     2281 agtgggtggt gcaaaggttg ccgtgccgac aggtccaacc cctttggatt caacccctcc
     2341 aggaggtggc cccctccctc tgactggcca ggaggaagcc agggctgtag agaaggaaaa
     2401 acccaaggct cggtcagagg actcggggct ggatggtgtg gtggctcgca cccccttgga
     2461 gcatgttacc ccaaagccag aaccgcactt ggcctcgccc aaacccacat gtatggtgcc
     2521 tccaatgcct catagtccag tgagtggaga tagtgtggag gaggatgaag aggaagagaa
     2581 gaaggtttgt ctcccaggct ttactggcct cgtcaactta gggaacaccc gcttcatgaa
     2641 tagtgtcatt cagtctctgt ccaacactcg ggagcttcgt gacttcttcc acgaccgatc
     2701 ctttgaggct gagattaact acaataaccc attggggact ggtgggcgtc tggctattgg
     2761 cttcgctgtg ctgctccggg ccctgtggaa gggcactcac caagcctttc aaccctccaa
     2821 gctaaaggcc attgtagcaa gcaaggccag ccagtttaca ggctacgcac agcatgatgc
     2881 ccaagagttc atggctttct tgttggatgg gctgcatgaa gacctgaatc ggatccaaaa
     2941 caaaccctac acagagactg tggactcaga tgggcgacct gacgaggtgg tggctgaaga
     3001 agcatggcag cggcacaaga tgaggaacga ctcattcatt gtggacctgt ttcagggcca
     3061 gtacaagtca aagctggtgt gccctgtgtg tgccaaggtc tccatcactt ttgacccgtt
     3121 cctttatctg ccggtaccct tgccacaaaa gcaaaaggtt ctccccatat attattttgc
     3181 cagagagccc catagcaaac ccatcaagtt cctggtgagc gtcagcaagg agaactccag
     3241 cgcaagcgaa gttttggagt ccctctctca gagtgtccac gtgaagcctg agagcctgcg
     3301 cctggctgag gtaattaaga atcgtttcca ccgtgttttc ttgccctccc actcactgga
     3361 tgctgtgtcg ccaacggacg tgctcctgtg ctttgagctg ctatccccag aattggctaa
     3421 ggagagggta gtggtgctag aggtgcagca gcgcccccag gtacccagca tccctatctc
     3481 caagtgtgca gcctgccagc ggaagcagca atcagaagat gaaaaactga agcgttgtac
     3541 ccgttgctac cgtgtgggct actgcaacca gttctgtcag aaaacccatt ggcctgacca
     3601 caaaggcctc tgccgccccg agaacattgg ctaccccttc ctggtcagtg tacctgcttc
     3661 acgcctcact tatgcccgtc ttgctcagct actagaaggt tatgcccggt actctgtgag
     3721 cgtattccaa ccacccttcc agcctggccg aatggctttg gaatcacaga gccctggctg
     3781 taccacgttg ctttcaacca gctctctgga ggctggggat agtgaaagag aacccattca
     3841 gccttctgag ctccagttgg tgactcctgt ggctgagggg gataccgggg ctcaccgcat
     3901 gtggccacct gctgatcggg gtcctgtgcc tagcaccagt ggaatttctt ccgagatgct
     3961 ggccagtggg cctatggaag ggtgttcctt gcttgctggt gagagggtgt ctcggcctga
     4021 agctgctgtg cctgggtacc agcactcacg tgagtctgtg agcgcccaca cgccccagtt
     4081 cttcatctat aaaattgatg cgtcaagccg tgagcagcgg ctggaggaca aaggggacac
     4141 tccactggag ctaggtgatg actgtagcct ggctctggtg tggcggaaca acgaacgcct
     4201 tcaggagttt gtgctggtgg cctccaagga gctggagtgt gctgaagacc caggctccgc
     4261 tggtgaggct gcccgtgctg gccacttcac cctggaccag tgcctcaatc tctttacacg
     4321 gcctgaagtg ctggcacctg aggaagcctg gtactgccca cagtgcaaac agcatcgaga
     4381 ggcctccaag cagctgctgc tgtggcgcct accaaatgtg ctcatcgtgc agctcaagcg
     4441 tttctccttt cgtagcttca tttggcgtga caagatcaat gacttggtgg agtttccagt
     4501 tcggaacctg gacttgagca agttctgtat tggtcagaaa gaggagcagc tgcctagcta
     4561 tgacctgtat gcagtcatca accactacgg aggcatgatc ggcggccact acactgcctg
     4621 tgcacggctg cccagtgacc gtagtagcca gcgcagtgac gtgggctggc gcttgtttga
     4681 tgacagcacg gtgacaacag tagacgagag ccaggtggtg acacgctatg cctatgtgct
     4741 cttctaccgt cggcggaact ctcctgtgga gaggcccccc agggcagctc acgctgaaca
     4801 ccacccagac ctaggccctg cagccgaggc tgctgccagc cagggactag gccctggcca
     4861 ggcccccgag gtggccccca cgcggacagc ccctgaacga ttcgtccccc ctgtggaccg
     4921 cccagccccc acgtacagca acatggagga ggtcgattag caggtccgtc gaaaacccgc
     4981 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5041 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5101 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5161 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5221 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5281 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5341 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5401 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5461 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5521 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5581 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5641 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5701 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5761 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5821 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5881 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5941 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6001 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6061 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6121 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6181 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6241 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6301 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6361 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6421 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6481 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6541 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6601 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6661 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6721 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6781 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6841 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6901 gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6961 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7021 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     7081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7201 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7321 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9361 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     9421 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9481 tgctccctgc ttgtgtgttg gaggtcgctg ag
//
