LOCUS       sequence-31196-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-AK
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(817..835)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      831..878
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc aattcgaaaa agcataattt aattcttatt
      121 ggagctccag gaagtgggaa aggtacccaa tgtgaattca tcaagaaaga gtatggattg
      181 gcacatttat ctactggtga tatgctgaga gaagcaataa aaaatggcac aaaaattggt
      241 ttggaagcga aaagtattat tgaatcaggg aactttgttg gagatgaaat tgtacttgga
      301 ttagttaagg aaaagtttga tttgggagta tgtgtaaatg ggtttgtctt ggacggattt
      361 ccaagaacaa ttccacaagc tgagggatta gcaaagatac taagtgaaat tggagattct
      421 ttaactagtg taatttactt tgaaattgac gattctgaga ttattgaaag aatttctgga
      481 agatgcactc acccagcatc tggaagaatt taccatgtaa agtataatcc tccaaagcag
      541 ccaggtatag atgatgtcac aggtgagcct cttgtatgga gagatgatga taatgcagaa
      601 gctgtgaagg ttcgtcttga tgtattccat aaacagactg ctccattagt gaagttttat
      661 gaagacctag gaatactaaa gagagtgaat gctaagctac ctcctaaaga agttacagag
      721 caaatcaaaa agatcttgtg atgacgaagc ttgcggccgc actcgaggat ccggctgcta
      781 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      841 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      901 gatat
//
