LOCUS       sequence-31199-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CyP8
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(769..787)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      783..830
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc aaactgaaaa agaaaggata cttacgcatc
      121 gttaccacgc aaggaagcct gaacattgaa ctgcatgccg atatggcacc gcgtgcgtgc
      181 gattcctttt tacgcctgtg tgctgtaaaa tattttgacg atacaatctt tcaccggtgc
      241 attcgtaatt ttatgatcca aggtgggcgc gccgaactgc gtcagccttc gaagaaaaaa
      301 gaggtgcaac agagcccgcg ctctattagt ggcttcccag gtggagcacc gtttgaggac
      361 gagttcgata accggttagt ccatcagggc attggtgttc tgtcaatggc gaatgatggt
      421 aaacattcca acctgagcga atttttcatc acttttaaat cgtgtgaaca tctgaataac
      481 aaacacacga ttttcggacg tgtggtaggc ggtttagacg tcttacgcca atgggagaag
      541 ctggaaacgg ataaaaagga taaaccgctg aagcctccaa aagttgagga aatcattgtg
      601 tttaagaatc cttttgaaga tgcccgtaaa gagatggagg atgaaaaacg cgaggaagag
      661 gaaaaggaaa aaaaaaagct ggagaacgca tgatgacgaa gcttgcggcc gcactcgagg
      721 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      781 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagga
//
