LOCUS       sequence-31217-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NEDD4
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             576..593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(646..664)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      660..707
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc atggcaactt gcgcggtgga ggtgttcggg
      121 ctcctggagg acgaggaaaa ttcacgaatt gtgagagtaa gagttatagc cggaataggc
      181 cttgccaaga aggatatatt gggagctagt gatccttacg tgagagtgac gttatatgac
      241 ccaatgaatg gagttcttac aagtgtgcaa acaaaaacca ttaaaaagag tttgaatcca
      301 aagtggaatg aagaaatatt attcagagtt catcctcagc agcaccggct tctttttgaa
      361 gtgtttgacg aaaaccgatt gacaagagat gatttcctag gtcaagtgga tgttccactt
      421 tatccattac cgacagaaaa tccaagattg gagagaccat atacatttaa ggattttgtt
      481 cttcatccaa gaagtcacaa atcaagagtt aaaggttatc tgagactaaa aatgacttat
      541 ttaccttgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      601 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      661 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      721 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      841 tttcttcccc ttcctttctc gccacgttcg ccggctttcc cccgtcaggc tctaaatcgg
      901 gggct
//
