LOCUS       sequence-31273-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK4 (2WEI)
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..20
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             33..53
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag ggcacctttg
       61 cagaaaggta caatattgta tgtatgcttg gcaaaggttc ttttggagaa gttttgaaat
      121 gtaaagacag aatcactcaa caagaatatg ctgtcaaggt tattaacaaa gctagtgcaa
      181 agaacaagga tactagtaca attttgagag aagttgagct tttaaagaag cttgatcatc
      241 caaatattat gaagctattc gagattttgg aagattcatc tagcttctac attgtaggag
      301 agctttacac aggaggagaa ctctttgatg aaattattaa gagaaaaaga tttagtgagc
      361 atgatgctgc tagaattatt aaacaagtat tttcaggaat tacttacatg cataaacata
      421 acattgtaca tagagattta aagccagaaa acatactttt agagtctaaa gaaaaagact
      481 gtgatattaa aattatagat tttggattat caacatgctt ccagcaaaat accaagatga
      541 aggatagaat aggaacagcc tactatatag ctccagaagt tttgagaggt acttatgatg
      601 agaaatgtga tgtatggtca gctggagtaa ttctatatat tctcttatct ggaacacctc
      661 cattctatgg aaaaaatgaa tatgatattt tgaagagagt cgagacagga aagtatgctt
      721 ttgaccttcc acaatggaga actatttctg atgatgccaa ggatttaata agaaagatgt
      781 taac
//
