LOCUS       sequence-31282-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-PMM
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attccctctg aatattttgt ttaactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcat gaaaagagtt cttttactct
      121 ttgacgttga cggaacgcta actcctcctc gactttgcca aacggacgaa atgagggctt
      181 tgataaagcg agcgcggggt gcagggtttt gtgtgggtac agttggagga tctgactttg
      241 caaagcaggt tgagcaactc ggacgtgacg tcctcactca gttcgattac gtatttgcag
      301 aaaatggtct actggcgtac cggaatggat tagagattca tcggcaaagt ttgttgaatg
      361 cacttggaaa cgaccgcatt gtaaaatttg ttaagaaaac gctgcgactc attgctgacc
      421 ttgatattcc ggtccagagg ggcacatttg tcgaatatag gaatggtatg ataaacgttt
      481 cgccgatagg acgcaactgc tcccaggctg agagagatga atttgaagtt tatgataacg
      541 aacacagagt ccgtgcaagc ttaattgcgg agctggagaa gagcttcccc gattttgggc
      601 tgaaatactc tattggtgga cagataagct ttgatgtctt tccagtaggc tgggacaaaa
      661 cctattgttt gcagtttgtt gaagacgact tcgaggagat ccactttttt ggcgataaga
      721 cccaagaagg tggaaatgac tacgaaattt acacggacaa gcggactatt gggcacaaag
      781 ttacttccta caaagatacc attgcagagg tggaaaaaat aattgccatg aagtgatgac
      841 gaagcttgcg gccgcactc
//
