LOCUS       sequence-312976-       10691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10TC)
FEATURES             Location/Qualifiers
     source          1..10691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1758..1772,1773..1778,1779..1795,1796..1802)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1778..1794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1810..1826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1845..1856
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1863..1889,1890..1892,1893..4379,4380..4382,
                     4383..5969)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6139..6930
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7053..7641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7542..7561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agggctttac aaagttcttt taaaagagaa tttagtaaag ccctaagtct tttaataaaa
       61 aattttttgt aaaaaagttg gcaaaaatag tttttgctat atatttatat ataagaaaaa
      121 attcaatact caaggagata aaaaatggaa aacttaaaca ataatccaca aaaaattaat
      181 caaataaata agcaaactaa ttttattggt tttgaaaatt ttatagaact tgataatcaa
      241 gttaatcaaa ctaaagaaaa aagtaaatta agtaagatag gtaagaaatt acccggaata
      301 agcagtcaag tctagtgcgg ccggcgcgcc aaataatgcc cgtgtccctg ttgaataggg
      361 acacggtttt ttaacaattt ttttattcct aggccttagt ggccggtacc cggggatcct
      421 tattactggc cgctttctag cctggatact tgtctggcta gttgcattag agagtcagct
      481 agagccctag ggctagctgt ttgtgtattc ggagccagtg tagtttttct ttttactaac
      541 gatacgggta ataattgatt gccttttaca gcctgacctt gagacaattg caaaagtctg
      601 tccacagagg tttgacctaa taatctaaaa tcttgtttga tagtagtaag tggaggtatg
      661 tagcaactag aatcttctgt atcatcataa cccacaactg aaatatcagc cccaactcta
      721 agtccacttt cagtaatagc tcgcatagct cctaaagcca tttgatcatt agcaactaac
      781 atagcagtag ggacaatgcc ttcatttaac atttgcattg tttgctgaaa tcctgacata
      841 gcagaccaat ctccttctct ttctgctatt ggttgtattt gatttcttgt taaatacttg
      901 tgccatcctg ctaatctcaa tctggcactt actgaactta agggacctgc aagtagtgca
      961 atttgttgat ggcctaatgc aacaaggtgt tcaactccta accttgttcc atcttcgtga
     1021 gaaaaaatta tagagtttat tggggtttgg tccgacacat ccaaaaacaa tgctggaaca
     1081 ttagtacatg ctgcttcaac tgcaatcgca tcttggtcat caagaggata attaattata
     1141 agtccactaa cacgttgagc taataaatta tgtactgctg ccttacaagc ctctacacct
     1201 gatctctcaa ccattgatac aacaacgctt gcaccaagtt gatcagctct cgatttaatt
     1261 gcggctacaa tttgactagg cgcatgtaat gcaagtgatg atgtggcaac tccaattaaa
     1321 agtgattgtt tccccgcaag ttgctgagca acgcgatttg gtatataatt caactctgcc
     1381 atagctgctt caaccttctc tcttgttttt gcagatacat gacttgcttg gttcacaact
     1441 ctcgaaaccg tttggtagct aaccccggca tattcagcaa catcatacaa agtaacaggt
     1501 ttcatggatc attcctccat gataaaattt aaatttctga ctttggcaaa tccttttgcc
     1561 tgttaaaaag aatagcacaa aaattaaaaa aaaagaaacg caaatagcaa tagcattaaa
     1621 aataattatt attatttatt tctttgatta aaatcaaaag cctttaatgt attttcttga
     1681 tctttcatgc gcttaacggt aaaatccaag gtattaagtt caccttccac ttttctctct
     1741 ctatcttctc tagaaaatca taaaaaattt atttgctttg tgagcggata acaattataa
     1801 cagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattac
     1861 atatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc ggatgggcgg
     1921 tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga aatacagacc
     1981 gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga gagacagcgg
     2041 aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtattt
     2101 gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc
     2161 gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg
     2221 gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa
     2281 aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc
     2341 atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg
     2401 atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc
     2461 ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa
     2521 gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga
     2581 atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag
     2641 aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg
     2701 cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta
     2761 ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc ctatcagctt
     2821 caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc
     2881 gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg
     2941 caggttatat tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt
     3001 tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa gatttgctgc
     3061 gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc
     3121 atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga
     3181 ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata
     3241 gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag
     3301 aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca aactttgata
     3361 aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg
     3421 tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc
     3481 tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa
     3541 ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat agtgttgaaa
     3601 tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa
     3661 ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg
     3721 ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg
     3781 ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg
     3841 gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat
     3901 tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg atccatgatg
     3961 atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt
     4021 tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga
     4081 ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg
     4141 ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc
     4201 gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc
     4261 ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa
     4321 gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatg
     4381 ccattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc ttaacgcgtt
     4441 ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc aaaaagatga
     4501 aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt
     4561 taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt atcaaacgcc
     4621 aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat agtcgcatga
     4681 atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat
     4741 ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca
     4801 attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct ttgattaaga
     4861 aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta
     4921 aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat ttcttttact
     4981 ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaac
     5041 gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt
     5101 ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag
     5161 tccagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg gacaagctta
     5221 ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt ccaacggtag
     5281 cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg
     5341 ttaaagagtt actagggatc acaattctgg aaagaagttc ctttgaaaaa aatccgattg
     5401 actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt aaactaccta
     5461 aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt gccggagaat
     5521 tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta tatttagcta
     5581 gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa ttgtttgtgg
     5641 agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt tctaagcgtg
     5701 ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa catagagaca
     5761 aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg aatcttggag
     5821 ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat acgtctacaa
     5881 aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat gaaacacgca
     5941 ttgatttgag tcagctagga ggtgactaat aaaagcttga tcagatctca gctttttttt
     6001 gaagtgcctg gcagtaagtt gaaaaaattt gaaataaact tggactatgt taataaaaaa
     6061 atacaaatta acgaacaaaa agatttgaat tattatataa tttagttaaa agcaatttta
     6121 aatgagggag gtttccatat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     6181 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     6241 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     6301 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     6361 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     6421 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     6481 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     6541 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     6601 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     6661 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     6721 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     6781 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     6841 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     6901 ggcgagatca ccaaggtagt cggcaaataa tgacgtcgcc ggagcggtcg agttctggac
     6961 cgaccggctc gggttctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7021 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7081 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7141 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7201 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7261 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7321 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7381 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7441 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7501 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7561 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7621 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7681 tgctggcctt ttgctcacat gttctttcct gcactagatc cccggcaagt tcatccttag
     7741 ggttttcata tctcttaaaa agtcaatttc tgcttgatta atttcttgtg gattattgtt
     7801 tttgcggttt ttcatttttt atctccttga gtattgaatt ttttcttata tataaatata
     7861 tagcaaaaac tatttttgcc aactttttta caaaaaattt tttattaaaa cacttagggc
     7921 tttgctaaat tctcttttaa aagaacttag taaagcccta tggatttaag aactgattat
     7981 acttacgtag taaaaatact acagatatta atagtataga ccatatcaaa atcataatca
     8041 atttatattt atctaaaatt gtagtccata caaaatacaa aaaaattagg tttttctaaa
     8101 ttctatttta aaagaactta gtaacaccta atctctaaat tacttacaaa attctaaaat
     8161 tagattttta gttcttcata tttctttaaa agttttttaa gaaaatcttt ttgattttca
     8221 aaaatttcat gcatcataaa actagtaaat ttggattttt ttttgtaaaa atcataactt
     8281 tcttgggttt taagttgaaa tcttaatggt tttattggat tttgcctaga tttttttaac
     8341 gccggacttt ctttatcttt tataaaatta taagaatttt taataccatt ttctattaaa
     8401 taatcttctt ttataagccc ctcttgcagt gcagttgcta atcttaaata cttatacgct
     8461 tgagtttttg ctacatcaaa gtcttttata aaatcaagaa aagttttata accatcaaat
     8521 ttataatatt gattatcttt tatttctttt aaaattttaa tattttccac cctgtggaat
     8581 atttcttttt tgaaattgtc atttaacttt tcctttagtg tcttgtaata agaaatttta
     8641 ctttcattaa tcattaaatt ttgttcaaca taagtatcta tcttttttgt taaaacttta
     8701 acaacacgtt ttttttccat aaaaattttc ctttattttt gcaacatttt ccttaattga
     8761 tataattgtt actattttag attttttaat atccacttaa gaattaatcc ctcactagaa
     8821 tattttttat tatagaattc atatacatgt ttttctaaat ctataaattt atcaacaatt
     8881 gctttattat agggtgtatc tatcttttct tttcttaata atcgtctcat attctttaaa
     8941 taacaacaaa cactgccggt tttaaaccta aattctatgt aatatatttt tgaaaaagta
     9001 tatacatttt caaccccgtt tatttcatac tttacaaaaa cattatttat tggttttcta
     9061 taaccatagt aaatgcctaa aaatttgtcc ccgtctttca taggatatag ataaagtttt
     9121 tgaatctctg atttatctaa taatttttca aaaaaaatta tacaccgctt ttgtttgtta
     9181 ctaactactc tatgcaatgc ctttaatatt tttgtgtgat atatattttt atcacctttt
     9241 ttttctttca gcaggaagat actttctttt ggaaatttaa ttttttgttc atttattctt
     9301 gcagccttaa gtccgatttt catatttaat ccttatatag cctttctatt taattctttt
     9361 aaactcaata aacttttttg gtttttcatt agttctaata attcgcaata atatgtgcaa
     9421 aacactttat tgtattctat tttcttttgg ttattcaaat attcttttat aattggcttt
     9481 agaagttcaa tatttacttc tttttttagt tgttcaataa ggatattgaa aatgtttgtt
     9541 tttaaatctt gataattttg ttgagaattt tctttttttc tttcaattga cttctctaat
     9601 ttacgcttta tattgtttaa atcgctatat ttatgacttt caataataaa atgaggctta
     9661 aatttgtaat tttcgtatat tttttggaaa cttgtttcta attgtttaga attatattca
     9721 tttttttcaa attctttttg agtgttgcat agaatttcct ttaatttttc ttgtttttct
     9781 ttcatacaag atttagtaaa atgtatattt ttatttttta gtaatttaat ttctattatt
     9841 tttatgattt taagtatttt gattatatta tctttatcaa tatttaaatt gaataaaatt
     9901 gtaagaaaaa ttgaaagaat ttttttacat aaaaagttac atttattgaa gtaatttctt
     9961 acttgatact tttctatttc attaatcttt ctttcttctt ttatattatt tttattattt
    10021 aaacactcct ttaaatctac actcccattt ttagtaaatc tatctttaag gccccttgaa
    10081 actctatttt gaaatcttaa atcttttttt tcttgaaagt atttatagat tttttggtaa
    10141 cattcttttt ttgaaaaatt aagttgatag tatatttcgg tccccaaatt tacccctaag
    10201 tgtttgtgat aattagttgt gactttaaat tctttttcta atttgtaaag gtaattttgc
    10261 agtgttttta gtttaacggg tgtttgacta tttcttttaa ggttttcatt aaagtaatag
    10321 agtatgtttt tttgtgtgta tttcttatat gttttattta tgtacgctag tgttgagata
    10381 aggactacta atttatactg taatttattg tagcaagttg tggttttact ggttttgttt
    10441 atattagata agttttccat ttatagctcc ttgaataccg gattttttat ctataaagta
    10501 tatatatcac aattttgatt taaaagtaaa ttcttttttt aaaaatacaa aaatttttta
    10561 taaattatat agaccatgat attgtattaa tactaattga ttttaattta aggttgaact
    10621 aaaataaaca tagttttgta gaaaataatt tttcattatt ttgctacttg atattggatc
    10681 gtaaaaatat t
//
