LOCUS       sequence-313011-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NLS-VP16-intN under the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJB004 CMV 3xFLAG-NLS-VP16-intN
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatcc
      121 gtcgacggtg gactgcaggc cccaccaacc gatgtttctt taggtgatga gttacatttg
      181 gacggtgagg acgtagctat ggcacatgca gatgccttag acgattttga cttggatatg
      241 ttaggagatg gagattcacc aggacctggt ttcacaccac acgattcagc tccatacggt
      301 gctttggaca tggccgattt cgaatttgaa cagatgttta ctgatgcctt gggtatagac
      361 gaatacggtg gtggaggtac cagaagcgga tactgcctgg acctgaagac ccaggtgcag
      421 acccctcagg gcatgaagga gatcagcaac atccaggtgg gcgacctggt gctgagcaac
      481 accggctaca acgaggtgct gaacgtgttc cccaagagca agaagaagag ctacaagatc
      541 accctggagg acggcaagga gatcatctgc agcgaggagc acctgttccc cacccagacc
      601 ggcgagatga acatcagcgg cggcctgaag gagggcatgt gcctgtacgt gaaggagtga
//
