LOCUS       sequence-313019-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NLS-DsDed-intN under the EF1-alpha
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM555 EF1-alpha 3xFLAG-NLS-DsDed-intN in TUPV2
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             139..813
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWEKVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(319..339)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     731..754
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatca
      121 ggctccggaa gtggctccat ggatagcact gagaacgtca tcaagccctt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggcaagccct acgagggcac ccagaccgcc aagctccagg tgaccaaggg cggccccctg
      301 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
      361 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
      421 aaggtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctccag
      481 gacggcacct tcatctacca cgtgaagttc atcggcgtga acttcccctc cgacggcccc
      541 gtaatgcaga agaaaactct gggctgggag ccctccaccg agcgcctgta cccccgcgac
      601 ggcgtgctga agggcgagat ccacaaggcg ctgaagctga agggcggcgg ccactacctg
      661 gtggagttca agtcaatcta catggccaag aagcccgtga agctgcccgg ctactactac
      721 gtggactcca agctggacat cacctcccac aacgaggact acaccgtggt ggagcagtac
      781 gagcgcgccg aggcccgcca ccacctgttc cagtctggtt ctggttctgg ttcaggatcc
      841 ggtggtggcg ggtctggagg aggaggtact ggtggaggtg gcactggtac cagaagcgga
      901 tactgcctgg acctgaagac ccaggtgcag acccctcagg gcatgaagga gatcagcaac
      961 atccaggtgg gcgacctggt gctgagcaac accggctaca acgaggtgct gaacgtgttc
     1021 cccaagagca agaagaagag ctacaagatc accctggagg acggcaagga gatcatctgc
     1081 agcgaggagc acctgttccc cacccagacc ggcgagatga acatcagcgg cggcctgaag
     1141 gagggcatgt gcctgtacgt gaaggagtga
//
