LOCUS       sequence-313157-       10593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of synaptotagmin 1 variant with self-cleaving C2 domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Vevea JD, Chapman ER
  TITLE     Acute disruption of the synaptic vesicle membrane protein 
            synaptotagmin 1 using knockoff in mouse hippocampal neurons.
  JOURNAL   eLife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469.
  PUBMED    32515733
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-06-9"; volume: "9. pii: 56469. doi"; pages: " 
            10.7554/eLife.56469"
COMMENT     Sequence Label: pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)
FEATURES             Location/Qualifiers
     source          1..10593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      479..488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2615..2638
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2684..3272
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2737..2757)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3155..3166)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3275..3291)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3276..3292)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3797..3977
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4003..4020)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4009..4233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4279..4707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4366..4385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4576..4597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4716..4736)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4721..5050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4901..5036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4963..4982
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5098..5145
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5164..5538
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5668..5789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5705..5724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5759..5778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5838..5854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5838..5854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5851..5873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5862..5878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5886..5892,5893..5910,5911..5916))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5931..5952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6069..6086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6240..6828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6320..6339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6999..7790,7791..7859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7622..7641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7860..7964)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8039..8058)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8230..8609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8611..8809
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8764..8784
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8827..9007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9054..9179
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9672..9905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10090..10134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10432..10549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
       61 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
      121 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
      181 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
      241 ctgacgtcac tcgccggtcc cccgtaaact ccccttcccg gccaccttgg tcgcgtccgc
      301 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
      361 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
      421 ggagtcgtgt cgtgcctgag agcgcaggta cggctagcgg atccggtacc gcgggcccgc
      481 caccatggtg agtgccagtc atcctgaggc cctggccgcc cctgtcacca ctgttgcgac
      541 ccttgtccca cacaatgcca ctgagccagc cagtcctggg gaagggaagg aagatgcctt
      601 ttccaagctg aagcagaagt ttatgaatga gctgcataaa attccattgc caccgtgggc
      661 cttaatagcc atagccatag ttgcggtcct tttagtcgta acctgctgct tttgtgtctg
      721 taagaaatgt ttgttcaaaa agaaaaacaa gaagaagggg aaggaaaagg gaggaaagaa
      781 cgccgctgac gtggtttgct gtcagatgag ttatattaac atgaaagacg tgaaagactt
      841 agggaagacc atgaaggatc aggcccttaa ggatgacgat gctgaaaccg gactgactga
      901 tggagaagaa aaggaagagc ccaaggaaga ggagaaactg ggaaagctcc aatattcact
      961 ggactatgac ttccagaata accagctgtt ggtgggaatc atccaggctg ctgaactgcc
     1021 cgccctggac atggggggta catccgatcc atacgtcaaa gtcttcctgc tgcctgacaa
     1081 aaagaagaaa tttgagacta aagtccaccg gaaaaccctc aatccagtct tcaatgaaca
     1141 atttactttc aaggtaccct actcggaatt aggtggcaaa accctggtga tggctgtgta
     1201 tgactttgat cgcttctcca agcacgacat catcggagag ttcaaagttc ctatgaacac
     1261 cgtggatttt ggccatgtga ccgaggagtg gcgcgatctc cagagcgctg agaaagaaga
     1321 gcaagagaaa ctgggtgaca tctgcttctc cctccgctac gtccctactg ccggcaaact
     1381 gactgttgtc attctggaag ccaagaacct gaagaagatg gatgtgggtg gcttatctga
     1441 tccctacgtg aagattcacc tgatgcagaa cggtaagagg ctgaagaaga aaaagacgac
     1501 gattaagaag aacacactca acccctacta caacgagtcc ttcagctttg aagttccgtt
     1561 cgagcaaatc cagaaagtgc aagtggtggt aactgttttg gactatgaca agattggcaa
     1621 gaacgacgcc atcggcaaag tcttcgttgg ttacaacagc actggggcgg agctgcgaca
     1681 ctggtcagac atgctggcca acccccggcg acccatcgca cagtggcaca ctctgcaggt
     1741 agaggaggag gtcgatgcca tgctggctgt caagaagggt tcagggtcta gtggcagctc
     1801 tggatcctcg gggtcagcag gcagtgctgc cggttccgga agcgggtcca gtggtagctc
     1861 aggctcttcc ggaagcggat ccggaagttc ggggtccagc ggttctagtg gttcagccgg
     1921 aagcgccgct ggaagtgggt caggatcatc tggcagatcg gggagtagcg gttcctcagg
     1981 ctgcgtggtg atcgtgggca ggatcgtgct gagcggaagc ggcaccagcg cccccatcac
     2041 cgcctacgcc cagcagacca ggggcctgct gggctgcatc atcaccagcc tgaccggcag
     2101 agacaagaac caggtggagg gcgaggtgca gatcgtgagc accgccaccc agaccttcct
     2161 ggccacctgc atcaacggag tgtgttggac cgtgtaccac ggcgccggca ccagaaccat
     2221 cgccagcccc aagggccccg tgatccagat gtacacaaac gtggaccagg acctggtggg
     2281 ctggcccgcc ccccagggca gcaggagcct gaccccctgc acatgcggca gcagcgacct
     2341 gtacctggtt accaggcacg ccgacgtgat ccccgtgagg aggaggggcg acagcagggg
     2401 cagcctgctg agccccaggc ccatcagcta cttgaaggga tctagcggag gccccctgct
     2461 gtgccccgcc ggccacgccg tgggcctgtt cagggccgcc gtgtgcacca ggggcgtggc
     2521 caaggccgtg gacttcatcc ccgtggagaa cctggagacc accatgagga gccccgtgtt
     2581 taccgacaac tcctctccac cagcaggatc cagtgactac aaggatgacg acgataaata
     2641 aggccgcact cctcagctag aattcgatat caagcttatc gataatcaac ctctggatta
     2701 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2761 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2821 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2881 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2941 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3001 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3061 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3121 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3181 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3241 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
     3301 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     3361 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     3421 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3481 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     3541 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     3601 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     3661 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     3721 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     3781 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3841 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3901 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3961 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4021 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4081 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4141 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4201 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4261 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4321 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4381 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4441 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4501 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4561 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4621 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4681 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4741 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4801 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4861 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4921 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4981 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5041 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     5101 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     5161 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     5221 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     5281 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     5341 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     5401 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     5461 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     5521 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     5581 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5641 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5701 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5761 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5821 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5881 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5941 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6001 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6061 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6901 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6961 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7021 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7081 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7141 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7201 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7261 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7321 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7381 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7441 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7501 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7561 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7621 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7681 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7741 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7801 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7861 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7921 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7981 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     8041 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     8101 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     8161 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     8221 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     8281 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     8341 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     8401 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     8461 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     8521 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     8581 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     8641 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     8701 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     8761 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
     8821 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8881 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8941 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9001 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     9061 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     9121 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     9181 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     9241 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     9301 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     9361 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     9421 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     9481 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     9541 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     9601 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     9661 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     9721 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     9781 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     9841 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     9901 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     9961 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    10021 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    10081 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    10141 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    10201 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    10261 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    10321 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    10381 tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag
    10441 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
    10501 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
    10561 cagggacagc agagatccag tttggttaat taa
//
