LOCUS       sequence-31316-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CYP2R1 (2OJD)
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(170..175,176..193,194..200)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    208..224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(279..284,285..300,301..307)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    315..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(374..379,380..395,396..402)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    410..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggttcattcc caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
       61 ttttgcacca ttcgatggtg tcctggcacg acaggtttcc cgactggaaa gcgggcagtg
      121 agcgcaacgc aattaatgta agttagctca ctcattaggc accccaggct ttacacttta
      181 tgcttccggc tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca
      241 ggatcgatcc atcgatgagc ttactcccca tccccctgtt gacaattaat catcggctcg
      301 tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagga tcagcttact
      361 ccccatcccc ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga
      421 taacaatttc acacaggaaa caggatccat cgatgcttag gaggtcatat ggctaagaaa
      481 acgaagcaga ggcggccgat gggcttcccc ccggggccgc cggggctgcc atttatcggc
      541 aacatctatt ccctggcagc ctcatccgag cttccccatg tctacatgag aaagcagagc
      601 caggtgtacg gagagatctt cagtttagat cttggaggca tatcaactgt ggttctaaat
      661 ggctatgatg tagtaaagga atgccttgtt catcaaagcg aaatttttgc agacagacca
      721 tgccttcctt tattcatgaa gatgacaaaa atgggaggct tactcaattc cagatatggc
      781 cgaggatggg ttgatcacag acgattagct gtaaacagtt ttcgatattt tggatatggc
      841 caaaagtc
//
