LOCUS       sequence-313232-        8794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependently expresses human ASCL1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Nemoto A, Kobayashi R, Yoshimatsu S, Sato Y, Kondo T, Yoo AS, 
            Shiozawa S, Okano H
  TITLE     Direct Neuronal Reprogramming of Common Marmoset Fibroblasts by 
            ASCL1, microRNA-9/9*, and microRNA-124 Overexpression.
  JOURNAL   Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 
            10.3390/cells10010006.
  PUBMED    33375083
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-12-22"; volume: "10(1). pii: cells10010006. doi"; pages:
            " 10.3390/cells10010006"
COMMENT     Sequence Label: CSIV-TRE-ASCL1
FEATURES             Location/Qualifiers
     source          1..8794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..74)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        246..625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        627..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             843..1023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1070..1195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1688..1921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2106..2150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2424..2541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2601..2915
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2609..2627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2645..2663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2680..2698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2716..2734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2752..2770
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2787..2805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2823..2841
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2888..2912
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2925..2949
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3667..3691)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3735..4323
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3788..3808)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4206..4217)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4560..4593)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4607..4787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4813..4830)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4819..5043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5089..5517
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5176..5195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5386..5407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5526..5546)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5531..5860
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5711..5846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5773..5792
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5908..5955
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5974..6348
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6478..6599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6515..6534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6569..6588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6648..6664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6648..6664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6661..6683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6672..6688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6696..6702,6703..6720,6721..6726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6741..6762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6879..6896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7050..7638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7130..7149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7809..8600,8601..8669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8432..8451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8670..8774)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
       61 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
      121 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
      181 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
      241 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      301 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      361 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      421 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      481 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      541 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      601 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      661 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      721 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      781 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
      841 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      901 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      961 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1021 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1081 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1141 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1201 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1261 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1321 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1381 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1441 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1501 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1561 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1621 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1681 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1741 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1801 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1861 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1921 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1981 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2041 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2101 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2161 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2221 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2281 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     2341 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcta
     2401 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2461 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2521 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagaaattc actttgaatt
     2581 cggccctttc gtcttcaatc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     2641 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
     2701 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     2761 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
     2821 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
     2881 taagcagagc tcgtttagtg aaccgtcaga tcgccgctag catcacaagt ttgtacaaaa
     2941 aagcaggctc caccatggaa agctctgcca agatggagag cggcggcgcc ggccagcagc
     3001 cccagccgca gccccagcag cccttcctgc cgcccgcagc ctgtttcttt gccacggccg
     3061 cagccgcggc ggccgcagcc gccgcagcgg cagcgcagag cgcgcagcag cagcagcagc
     3121 agcagcagca gcagcagcag gcgccgcagc tgagaccggc ggccgacggc cagccctcag
     3181 ggggcggtca caagtcagcg cccaagcaag tcaagcgaca gcgctcgtct tcgcccgaac
     3241 tgatgcgctg caaacgccgg ctcaacttca gcggctttgg ctacagcctg ccgcagcagc
     3301 agccggccgc cgtggcgcgc cgcaacgagc gcgagcgcaa ccgcgtcaag ttggtcaacc
     3361 tgggctttgc cacccttcgg gagcacgtcc ccaacggcgc ggccaacaag aagatgagta
     3421 aggtggagac actgcgctcg gcggtcgagt acatccgcgc gctgcagcag ctgctggacg
     3481 agcatgacgc ggtgagcgcc gccttccagg caggcgtcct gtcgcccacc atctccccca
     3541 actactccaa cgacttgaac tccatggccg gctcgccggt ctcatcctac tcgtcggacg
     3601 agggctctta cgacccgctc agccccgagg agcaggagct tctcgacttc accaactggt
     3661 tctaggaccc agctttcttg tacaaagtgg tgatgctacc ggtctcgaaa catcgaggga
     3721 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3781 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3841 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3901 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3961 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4021 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4081 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4141 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4201 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4261 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4321 cgcatcgata ccgtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     4381 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     4441 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     4501 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatca
     4561 taacttcgta tagcatacat tatacgaagt tattccggac tgtactgggt ctctctggtt
     4621 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4681 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4741 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     4801 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4861 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4921 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4981 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5041 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5101 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5161 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5221 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5281 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5341 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5401 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5461 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     5521 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5581 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5761 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     5821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     5881 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     5941 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     6001 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     6061 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     6121 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     6181 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     6241 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     6301 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     6361 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6421 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6481 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6541 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6601 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6661 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6721 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6781 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6841 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8761 ataggggttc cgcgcacatt tccccgaaaa gtgc
//
