LOCUS       sequence-313234-        7118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7118)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..7118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        55..358
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        359..562
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     513..533
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3061..3070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3067..3777
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3212..3230)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3480..3499
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3900..4021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3937..3956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3991..4010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4028..4483)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4165..4186)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4377..4396
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4510..4614
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4616..4973
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4616..4807
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4639..4659)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4824..4959
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5008..5802
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5008..5802
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5062..5081)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5672..5691
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5990..6009)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6034..6081
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6410..6998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6899..6918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
       61 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      121 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      181 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      241 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      301 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      361 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      421 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      481 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      541 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      601 tcagatctcg agaccgccat gaatttacaa ccaattttct ggattggact gatcagttca
      661 gtttgctgtg tgtttgctca aacagatgaa aatagatgtt taaaagcaaa tgccaaatca
      721 tgtggagaat gtatacaagc agggccaaat tgtgggtggt gcacaaattc aacattttta
      781 caggaaggaa tgcctacttc tgcacgatgt gatgatttag aagccttaaa aaagaagggt
      841 tgccctccag atgacataga aaatcccaga ggctccaaag atataaagaa aaataaaaat
      901 gtaaccaacc gtagcaaagg aacagcagag aagctcaagc cagaggatat tactcagatc
      961 caaccacagc agttggtttt gcgattaaga tcaggggagc cacagacatt tacattaaaa
     1021 ttcaagagag ctgaagacta tcccattgac ctctactacc ttatggacct gtcttactca
     1081 atgaaagacg atttggagaa tgtaaaaagt cttggaacag atctgatgaa tgaaatgagg
     1141 aggattactt cggacttcag aattggattt ggctcatttg tggaaaagac tgtgatgcct
     1201 tacattagca caacaccagc taagctcagg aacccttgca caagtgaaca gaactgcacc
     1261 agcccattta gctacaaaaa tgtgctcagt cttactaata aaggagaagt atttaatgaa
     1321 cttgttggaa aacagcgcat atctggaaat ttggattctc cagaaggtgg tttcgatgcc
     1381 atcatgcaag ttgcagtttg tggatcactg attggctgga ggaatgttac acggctgctg
     1441 gtgttttcca cagatgccgg gtttcacttt gctggagatg ggaaacttgg tggcattgtt
     1501 ttaccaaatg atggacaatg tcacctggaa aataatatgt acacaatgag ccattattat
     1561 gattatcctt ctattgctca ccttgtccag aaactgagtg aaaataatat tcagacaatt
     1621 tttgcagtta ctgaagaatt tcagcctgtt tacaaggagc tgaaaaactt gatccctaag
     1681 tcagcagtag gaacattatc tgcaaattct agcaatgtaa ttcagttgat cattgatgca
     1741 tacaattccc tttcctcaga agtcattttg gaaaacggca aattgtcaga aggagtaaca
     1801 ataagttaca aatcttactg caagaacggg gtgaatggaa caggggaaaa tggaagaaaa
     1861 tgttccaata tttccattgg agatgaggtt caatttgaaa ttagcataac ttcaaataag
     1921 tgtccaaaaa aggattctga cagctttaaa attaggcctc tgggctttac ggaggaagta
     1981 gaggttattc ttcagtacat ctgtgaatgt gaatgccaaa gcgaaggcat ccctgaaagt
     2041 cccaagtgtc atgaaggaaa tgggacattt gagtgtggcg cgtgcaggtg caatgaaggg
     2101 cgtgttggta gacattgtga atgcagcaca gatgaagtta acagtgaaga catggatgct
     2161 tactgcagga aagaaaacag ttcagaaatc tgcagtaaca atggagagtg cgtctgcgga
     2221 cagtgtgttt gtaggaagag ggataataca aatgaaattt attctggcaa attctgcgag
     2281 tgtgataatt tcaactgtga tagatccaat ggcttaattt gtggaggaaa tggtgtttgc
     2341 aagtgtcgtg tgtgtgagtg caaccccaac tacactggca gtgcatgtga ctgttctttg
     2401 gatactagta cttgtgaagc cagcaacgga cagatctgca atggccgggg catctgcgag
     2461 tgtggtgtct gtaagtgtac agatccgaag tttcaagggc aaacgtgtga gatgtgtcag
     2521 acctgccttg gtgtctgtgc tgagcataaa gaatgtgttc agtgcagagc cttcaataaa
     2581 ggagaaaaga aagacacatg cacacaggaa tgttcctatt ttaacattac caaggtagaa
     2641 agtcgggaca aattacccca gccggtccaa cctgatcctg tgtcccattg taaggagaag
     2701 gatgttgacg actgttggtt ctattttacg tattcagtga atgggaacaa cgaggtcatg
     2761 gttcatgttg tggagaatcc agagtgtccc actggtccag acatcattcc aattgtagct
     2821 ggtgtggttg ctggaattgt tcttattggc cttgcattac tgctgatatg gaagctttta
     2881 atgataattc atgacagaag ggagtttgct aaatttgaaa aggagaaaat gaatgccaaa
     2941 tgggacacgg gtgaaaatcc tatttataag agtgccgtaa caactgtggt caatccgaag
     3001 tatgagggaa aaggtagcgc atccgggggt gcgtcaggcg gcagcggatc tggaccggtc
     3061 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     3121 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     3181 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     3241 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     3301 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     3361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     3421 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     3481 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     3541 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     3601 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     3661 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3721 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaagc
     3781 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     3841 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     3901 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3961 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4021 aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4081 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4141 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4201 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4261 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4321 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4381 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4441 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     4501 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4561 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     4621 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4681 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4741 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4801 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4861 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4921 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     4981 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5041 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5101 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5161 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     5221 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5281 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5341 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5401 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5461 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5521 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5581 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5641 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5701 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5761 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5821 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5881 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5941 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     6001 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     6061 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     6121 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     6181 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     6241 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7081 attctgtgga taaccgtatt accgccatgc attagtta
//
