LOCUS       sequence-313245-        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of ""extended"" membrane scaffold protein 
            (MSP1E1D1) for Nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ritchie TK, Grinkova YV, Bayburt TH, Denisov IG, Zolnerciks JK, 
            Atkins WM, Sligar SG
  TITLE     Chapter 11 - Reconstitution of membrane proteins in phospholipid 
            bilayer nanodiscs.
  JOURNAL   Methods Enzymol. 2009;464:211-31. doi: 
            10.1016/S0076-6879(09)64011-8.
  PUBMED    19903557
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2009"; volume: "464:211-31. doi"; pages: " 
            10.1016/S0076-6879(09)64011-8"
COMMENT     Sequence Label: pMSP1E1D1
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             105..125
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             784..801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(854..872)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      868..915
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1040..1059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttggaggaa attccctcta gaataatttt gtttaacttt aagaaggaga tataccatgg
       61 gtcatcatca tcatcatcat cacgattatg atattcctac tactgagaat ttgtattttc
      121 agggttctac cttcagtaaa cttcgcgaac aactgggccc cgtgacgcag gaattctggg
      181 acaacctgga aaaagaaacc gagggactgc gtcaggaaat gtccaaagat ttagaagagg
      241 tgaaggccaa ggttcagcca tatctcgatg actttcagaa aaaatggcag gaagagatgg
      301 aattatatcg tcaaaaggtg gaaccatatc tcgatgactt tcagaaaaaa tggcaggaag
      361 agatggaatt atatcgtcaa aaggtggaac cgctgcgtgc ggaactgcaa gagggggcac
      421 gccaaaaact ccatgagctc caagagaagc tcagcccatt aggcgaagaa atgcgcgatc
      481 gcgcccgtgc acatgttgat gcactccgga ctcatttggc gccgtattcg gatgaacttc
      541 gccagcgttt ggccgcacgt ctcgaggcgc tgaaagaaaa cgggggtgcc cgcttggctg
      601 agtaccacgc gaaagcgaca gaacacctga gcaccttgag cgaaaaagcg aaaccggcgc
      661 tggaagatct acgccagggc ttattgcctg ttcttgagag ctttaaagtc agttttctgt
      721 cagctctgga agaatatact aaaaagctga atacccagta ataagcttgc ggccgcactc
      781 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      841 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      901 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
      961 gtagcggcgc attaagcgcg gcggggggtg gtggttacgc gcagcgtgac cgctacactt
     1021 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1081 ggctttcccg tcagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1141 gcacctcg
//
