LOCUS       sequence-313303-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cell morphology marker construct. Contains a near infrared 
            fluorescent protein miRFP and six FLAG tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, 
            Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao 
            CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
  TITLE     Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling 
            Network Dynamics.
  JOURNAL   Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035.
  PUBMED    33232692
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Nov 17. pii: S0092-8674(20)31399-4. doi: 
            10.1016/j.cell.2020.10.035."
COMMENT     Sequence Label: pAAV-hSyn-miRFP-6xFLAG
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             31..54
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1051..1074
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1078..1101
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1105..1128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggattaca aagatgacga tgacaaaggc gactacaagg acgatgacga caaaggagac
       61 tacaaggatg atgacgacaa aggtggagga ggaggtatgg tcgcaggaca cgcaagtgga
      121 agccccgact ttggaaccgc cgatccgagc gactgtgaga gagaagaaat ccatctggcc
      181 ggatccatcc agccacacgg aacactgctg gtggtctccg agcctgacca tagaatcatt
      241 caggcatctg ccaacgccgc tgaattcctg aatctgggaa gtgtgctggg agtccccctg
      301 gctgagatcg acggggatct gctgatcaag attctgcccc acctggaccc taccgcagaa
      361 ggaatgccag tggccgtcag gtgccgcatt ggaaacccct ccacagagta cgatggactg
      421 atgcatcgac cacctgaagg aggactgatc attgagctgg aacgggctgg gccacccatt
      481 gatctgagcg gaacactggc tcccgcactg gagcgaattc gaactgcagg atccctgagg
      541 gctctgtgcg acgatactgc actgctgttc cagcagtgta ccggctacga ccgagtgatg
      601 gtctatcggt tcgatgaaca ggggcacgga gaggtgtatt cagaaataca tgtgactgga
      661 ctggagagct actttggcaa caggtatcct agctccctcg tgccacagat ggcccggaga
      721 ctgtacgaaa gacagagggt gcgcgtcctg gtggatgtct cttatcagcc tgtgccactg
      781 gagccaagac tgagtcccct gactggcagg gacctggata tgtcagggtg ttttctgcgc
      841 tctatgagtc ctactcacct gcagttcctg aagaatatgg gcgtgcgggc aacactggtg
      901 gtcagcctgg tggtcggagg caaactgtgg gggctggtga tctgccacca ttatctgcca
      961 cgcttcatcc attttgagct gcgagcaatt tgtgagctgc tggccgaagc tatcgcaacc
     1021 cgcattacag ccctgggagg tggaggagga gattacaaag atgatgatga taagggagat
     1081 tacaaggatg atgacgataa gggagactac aaggatgatg atgacaagta a
//
