LOCUS       sequence-313309-       15903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level M, P1 Golden Gate vector. 
            OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15903)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 15903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: HS_NAM-B1
FEATURES             Location/Qualifiers
     source          1..15903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..56
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      144..854
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1149..1940
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2281..2869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2770..2789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3205..4353
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4499..4523
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4623..4875)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          5575..5764
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             complement(6646..6663)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(6666..7508)
                     /label=Act1 promoter
                     /note="rice actin promoter"
     terminator      complement(7542..7790)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     intron          8591..8779
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     promoter        9694..11685
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    complement(11697..11730)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      13771..14023
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(14036..14069)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      15610..15862
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
       61 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
      121 ttcgtccatt tgtatgtgcc agccgccttt gcgacgctca ccgggctggt tgccctcgcc
      181 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
      241 gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
      301 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
      361 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
      421 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
      481 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
      541 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
      601 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
      661 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
      721 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc
      781 cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac
      841 cccaaaaatg gcagcgctgg ccaattcccg aggcacgaac ccagtggaca taagcctgtt
      901 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
      961 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1021 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     1081 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     1141 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     1201 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     1261 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
     1321 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     1381 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     1441 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     1501 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     1561 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     1621 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     1681 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     1741 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     1801 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     1861 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtgaaa ggcgagatca
     1921 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
     1981 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
     2041 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
     2101 atatatcatg ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2161 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2221 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2281 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2341 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2401 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2461 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2521 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2581 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2641 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2701 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2761 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2821 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2881 cgcggccttt ttacggttcc tggcagatcc tagatgtggc gcaacgatgc cggcgacaag
     2941 caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc
     3001 aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac
     3061 gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg
     3121 gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc
     3181 ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa
     3241 gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc
     3301 atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc
     3361 aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc
     3421 gtggagcgtt cgcgtcgtct tgaacaggag gcggcaggtt tggcgaagtc gatgaccatc
     3481 gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa
     3541 caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa
     3601 atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac
     3661 gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg
     3721 caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag
     3781 ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc
     3841 cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg
     3901 atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg
     3961 atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc
     4021 cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc
     4081 gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg
     4141 tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc
     4201 aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc
     4261 gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg
     4321 gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg
     4381 ggttcagcag cccctgctcg gatctgttgg accggacagt agtcatggtt gatgggctgc
     4441 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     4501 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     4561 cgtttttaat gtactggggt tgaacactct gtgggtctca tgccgaattc ggatccagcg
     4621 tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     4681 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     4741 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     4801 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     4861 aatgtttgaa cgatctgctt gacaagccta ttcctttgcc ctcggacgag tgctggggcg
     4921 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
     4981 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
     5041 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
     5101 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
     5161 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
     5221 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
     5281 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
     5341 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
     5401 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
     5461 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
     5521 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
     5581 ttatcatcat catcatagac acacgaaata aagtaatcag attatcagtt aaagctatgt
     5641 aatatttaca ccataaccaa tcaattaaaa aatagatcag tttaaagaaa gatcaaagct
     5701 caaaaaaata aaaagagaaa agggtcctaa ccaagaaaat gaaggagaaa aactagaaat
     5761 ttacctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     5821 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     5881 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     5941 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     6001 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     6061 gaaacttctc gacagacgtc gcggtgagtt caggcttttt cattggcttc tacctacaaa
     6121 aaagctccgc acgaggctgc atttgtcaca aatcatgaaa agaaaaacta ccgatgaaca
     6181 atgctgaggg attcaaattc tacccacaaa aagaagaaag aaagatctag cacatctaag
     6241 cctgacgaag cagcagaaat atataaaaat ataaaccata gtgccctttt cccctcttcc
     6301 tgatcttgtt tagcatggcg gaaattttaa accccccatc atctccccca acaacggcgg
     6361 atcgcagatc tacatccgag agccccattc cccgcgagat ccgggccgga tccacgccgg
     6421 cgagagcccc agccgcgaga tcccgcccct cccgcgcacc gatctgggcg cgcacgaagc
     6481 cgcctctcgc ccacccaaac taccaaggcc aaagatcgtg tccgagacgg aaaaaaaaaa
     6541 cggagaaaga aagaggagag gggcggggtg gttaccggcg cggcggcggc ggagggggag
     6601 gggggaggag ctcgtcgtcc ggcagcgagg ggggaggagg tggaggtggt ggtggtggtg
     6661 gtggtagggt tggggggatg ggaggagagg ggggggtatg tatatagtgg cgatgggggg
     6721 cgtttctttg gaagcggagg gagggccggc ctcgtcgctg gctcgcgatc ctcctcgcgt
     6781 ttccggcccc cacgacccgg acccacctgc tgttttttct ttttcttttt tttctttctt
     6841 tttttttttt tggctgcgag acgtgcggtg cgtgcggaca actcacggtg atagtggggg
     6901 ggtgtggaga ctattgtcca gttggctgga ctggggtggg ttgggttggg ttgggttggg
     6961 ctgggcttgc tatggatcgt ggatagcact ttgggcttta ggaactttag gggttgtttt
     7021 tgtaaatgtt ttgagtctaa gtttatcttt tatttttact agaaaaaata cccatgcgct
     7081 gcaacggggg aaagctattt taatcttatt attgttcatt gtgagaattc gcctgaatat
     7141 atatttttct caaaaattat gtcaaattag catatgggtt tttttaaaga tatttcttat
     7201 acaaatccct ctgtatttac aaaagcaaac gaacttaaaa cccgactcaa atacagatat
     7261 gcatttccaa aagcgaataa acttaaaaac caattcatac aaaaatgacg tatcaaagta
     7321 ccgacaaaaa catcctcaat ttttataata gtagaaaaga gtaaatttca ctttgggcca
     7381 ccttttatta ccgatatttt actttatacc accttttaac tgatgttttc acttttgacc
     7441 aggtaatctt acctttgttt tattttggac tatcccgact ctcttctcaa gcatatgaat
     7501 gacctcgagt atgctagctc cgcaagaatt caagcttagc gcttatcttt aatcatattc
     7561 catagtccat accatagcac atacagtagt tatatgctgc agaagagatc caacaaaaca
     7621 ttcacaatgg attatagaaa catttgttta ttcattataa tgagatctta cattcattta
     7681 atattagaaa aagccacaaa ttcataacac aacaagccaa gaaaaaaaca caaacttaag
     7741 cacacaagct ttttatttga cacaccaaat atttcatctt catcttcata taagcctaat
     7801 cgccatcttc cagcaggcgc accattgccc ctgtttcact atccaggtta cggatatagt
     7861 tcatgacaat atttacattg gtccagccac cagcttgcat gatctccggt attgaaactc
     7921 cagcgcgggc catatctcgc gcggctccga cacgggcact gtgtccagac caggccaggt
     7981 atctctgacc agagtcatcc ttagcgccgt aaatcaatcg atgagttgct tcaaaaatcc
     8041 cttccagggc gcgagttgat agctggctgg tggcagatgg cgcggcaaca ccattttttc
     8101 tgacccggca aaacaggtag ttattcggat catcagctac accagaaacg gaaatccatc
     8161 gctcgaccag tttagttacc cccaggctaa gtgccttctc tacacctgcg gtgctaacca
     8221 gcgttttcgt tctgccaata tggattaaca ttctcccacc gtcagtacgt gagatatctt
     8281 taaccctgat cctggcaatt tcggctatac gtaacagggt gttataagca atccccagaa
     8341 atgccagatt acgtatatcc tggcagcgat cgctattttc catgagtgaa cgaacctggt
     8401 cgaaatcagt gcgttcgaac gctagagcct gttttgcacg ttcaccggca tcaacgtttt
     8461 cttttcggat ccgccgcata accagtgaaa cagcattgct gtcacttggt cgtggcagcc
     8521 cggaccgacg atgaagcatg tttagctggc ccaaatgttg ctggatagtt tttactgcca
     8581 gaccgcgcgc ctgcacatca acaaattttg gtcatatatt agaaaagtta taaattaaaa
     8641 tatacacact tataaactac agaaaagcaa ttgctatata ccacattctt ttattttgaa
     8701 aaaaatattt gaaatattat attactacta attaatgata attattatat atatatcaaa
     8761 ggtagaagca gaaacttacc tgaagatata gaagataatc gcgaacatct tcaggttctg
     8821 cgggaaacca tttccggtta ttcaacttgc accatgccgc ccacgaccgg caaacggaca
     8881 ggagcatttt ccaggtatgc tcagaaaacg cctggcgatc cctgaacatg tccatcaggt
     8941 tcttgcgaac ctcatcactc gttgcatcga ccggtaatgc aggcaaattt tggtgtacgg
     9001 tcagtaaatt ggacattggt cggtgttgct tgctttggtt tgtagctcgc gttgaaatta
     9061 atctgtatcg gattgtaggt aggatgttgt tcgcttttgc ttgacgctgg cttgagttgg
     9121 gatggagggc gatggaaggt gagccactac ttatagcatc gtaggtactg atcttgaaga
     9181 atctcgagga ctgtccaatc gacgagtgtt tgtttctctt tcgctttcca ggtaggctgt
     9241 gagccattcg ttctttcctg ttcccaaccc tcgaagggtc tatcgtcttg caggggactc
     9301 acgaacctgc cgttagggcg ccttctccct tttttttttg ttaataattt ttttgtgaga
     9361 tttttctata aataattgta taattccagc aagggggcga ctagtctaga ttcaagaatg
     9421 ggttgtgggg gccgtttcca gaatagcatg attaacagta caggttatct gtcattaaag
     9481 ttacagcgag caagtacaat agttaaacgt aaataaatac tactccctcc gtcctaaaat
     9541 aaatgtctca agtttagtac aattttatat taaagttagt ataaagttca gacacttatt
     9601 ttaaaacaga aagagtacta cacaatgacg cagctagcgg gtggtccggg tggtccatgc
     9661 accatcttaa aattctccac tagaattcga gctcggaggt gcagcgtgac ccggtcgtgc
     9721 ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt
     9781 tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact
     9841 ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa
     9901 tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt
     9961 ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat
    10021 acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact
    10081 aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact
    10141 ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa
    10201 aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg
    10261 agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa
    10321 ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct
    10381 ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa
    10441 ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg
    10501 gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc
    10561 cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc
    10621 acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg
    10681 ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg
    10741 ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat
    10801 ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta
    10861 acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga
    10921 tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt
    10981 tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt
    11041 tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac
    11101 tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac
    11161 gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt
    11221 ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt
    11281 gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat
    11341 ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg
    11401 gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata
    11461 catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa
    11521 taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag
    11581 cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt
    11641 ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcaggtact gacctaataa
    11701 cttcgtatag catacattat acgaagttat attaagggtt gaatgggtca gtcccttatg
    11761 ttacgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc
    11821 agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa
    11881 agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt
    11941 aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg taagtcttac
    12001 tctctctttt ttggtctgta tttttaattt tttgaagtat actatttgta ctgacgctaa
    12061 taatcttttt tcaggttggg caggccagcg tatcgtgctg cgtttcgatg cggtcactca
    12121 ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg gctatacgcc
    12181 atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg
    12241 tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta ccgacgaaaa
    12301 cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggaa tccatcgcag
    12361 cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt
    12421 cgcgcaagac tgtaaccacg cgtctgttga ctggcaggta cttcatgctt caacgtgtaa
    12481 cttaagagat actgtgtgaa attttatatt tccatacatt tgcttgacct ttgctttttg
    12541 tcaatttttt tccccttaca ggtggtggcc aatggtgatg tcagcgttga actgcgtgat
    12601 gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca agtggtgaat
    12661 ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc
    12721 cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc
    12781 gaacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg tcatgaagat
    12841 gcggacttgc gtggcaaagg attcgataac gtgctgatgg tgcacgacca cgcattaatg
    12901 gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga agagatgctc
    12961 gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt cggctttaac
    13021 ctctctttag gcattggttt cgaagcgggc aacaagccga aagaactgta cagcgaagag
    13081 gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt
    13141 gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga tacccgtccg
    13201 caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg
    13261 cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac catcagcgat
    13321 ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag cggcgatttg
    13381 gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa actgcatcag
    13441 ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc aatgtacacc
    13501 gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg cgtctttgat
    13561 cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc gacctcgcaa
    13621 ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg caaaccgaag
    13681 tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga aaaaccgcag
    13741 cagggaggca aacaatgagc ttgtcaagca gatcgttcaa acatttggca ataaagtttc
    13801 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
    13861 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
    13921 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
    13981 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgacgctg acctaataac
    14041 ttcgtatagc atacattata cgaagttata ttaagggttg aatgggcagc tccgactcat
    14101 cttccggctc ggcgcaaaaa gcaacgcggt atcaccatca gcatcagccg ccgcctccgc
    14161 agcggggctc ggcgccggag ctcccgccgg gcttccggtt ccacccgacg gacgaggagc
    14221 tggtggtgca ctacctcaag aagaaggccg acaaggcgcc gctccccgtc aacatcatcg
    14281 ccgaggtgga tctctacaag ttcgacccat gggagctccc cggtatgtta tgtctatctc
    14341 gtcggccggc cgtgcttact ttatcaagcg ccgcaaattt taggtgcaat taaataatcg
    14401 aataatccat ccatctcatg cttatactcc tgtgcacaag tagtattttt atattcttcc
    14461 agtacacatg tgtgtagatg gtttatgtat gtgatcctgt cgtgcttgtt catgcgctcg
    14521 ggatccggat ccatcagaga aggcgaccat cggggagcag gagtggtact tcttcagccc
    14581 gcgcgaccgc aagtacccca acggcgcgcg gccgaaccgg gcggcgacgt cggggtactg
    14641 gaaggccacc ggcacggaca agcctatcct ggcctcgggg acggggtgcg gcctggtccg
    14701 ggagaagctc ggcgtcaaga aggcgctcgt gttctaccgc gggaagccgc ccaagggcct
    14761 caaaaccaac tggatcatgc acgagtaccg cctcaccgac gcatctggct ccaccaccgc
    14821 caccaaccga ccgccgccgg tgaccggcgg gagcagggct gctgcctctc tcagggtacg
    14881 tacacgtgtc gatcgcacgg tctagcagta tttaattgct ctccagctta attagggtat
    14941 tgttgatggt tgatgaagtt ggacgactgg gtgctgtgcc gcatctacaa gaagatcaac
    15001 aaggccgcgg ccggcgatca gcagaggaac acggagtgcg aggactccgt ggaggacgcg
    15061 gtcaccgcgt acccgctcta tgccacggcg ggcatgaccg gtgcaggtgc gcatggcagc
    15121 aactacgctt caccttcact gctccatcat caggacagcc atttcctgga cggcctgttc
    15181 acagcagacg acgccggcct ctcggcgggc gccacctcgc tgagccacct agcagcggcg
    15241 gcgagggcaa gcccggctcc gaccaaacag tttctcgccc cgtcatcgtc gaccccgttc
    15301 aactggctcg atgcgtcacc agtcggcatc ctcccacagg caaggaattt tcctgggttt
    15361 aacaggagca gaaacgtcgg caatatgtcg ctgtcatcga cggccgacat ggctggcgca
    15421 gtggacaacg gtggaggcaa tgcggtgaac gccatgtcta cctatcttcc cgtgcaagac
    15481 gggacgtacc atcagcagca tgtcatcctc ggcgctccgc tggtgccaga agccgccgcc
    15541 gccacctctg gattccagca tcccgttcaa atatccggcg tgaactggaa tccctgagct
    15601 tgtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
    15661 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
    15721 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
    15781 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
    15841 gtgtcatcta tgttactaga tcgacgcttt actgagaccg aggatgcaca tgtgaccgag
    15901 gga
//
